Behavioural risks in female dogs with minimal lifetime exposure to gonadal hormones


Autoři: Melissa Starling aff001;  Anne Fawcett aff001;  Bethany Wilson aff001;  James Serpell aff002;  Paul McGreevy aff001
Působiště autorů: Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia aff001;  School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0223709

Souhrn

Spaying of female dogs is a widespread practice, performed primarily for population control. While the consequences of early spaying for health are still being debated, the consequences for behaviour are believed to be negligible. The current study focused on the reported behaviour of 8981 female dogs spayed before 520 weeks (ten years) of life for reasons other than behavioural management, and calculated their percentage lifetime exposure to gonadal hormones (PLGH) as a proportion of their age at the time of being reported to the online Canine Behavioral Assessment and Research Questionnaire (C-BARQ). We found that 23 behaviours differed between entire and spayed dogs, of which 12 were associated with PLGH and 5 with age-at-spay (AAS). Two behaviours, chewing and howling, were significantly more likely in dogs with longer PLGH. In contrast, longer PLGH was associated with significantly reduced reporting of 10 (mostly unwelcome) behaviours. Of these, one related to fearfulness and three to aggression. The current data suggest that dogs’ tendency to show numerous behaviours can be influenced by the timing of spaying. They indicate how female dog behaviour matures when gonadal hormones are allowed to have their effect. The differences reported here between undesirable behaviours of spayed and entire dogs were in the range of 5.33% and 7.22%, suggesting that, for some dogs, partial or complete denial of maturation may reduce howling and chewing and improve retrieval and recall, but have other undesirable consequences. Veterinarians may take these data into account to discuss the risks and benefits of spaying with clients, and the timing of the procedure.

Klíčová slova:

Aggression – Animal behavior – Behavior – Dogs – Pets and companion animals – Sex hormones – Veterinarians – Gonadectomy


Zdroje

1. Animal Medicines Australia. Pet Ownership in Australia 2016. Research N, Communications N, editors. Newgate Communications; 2016 p. 66.

2. Pet Food Manufacturer's Association. Dog population 2018. PFMA, editor. Pet Population 2018. 2018.

3. American Pet Products Association. 2017–2018 APPA National Pet Owners Survey. In: americanpetproducts.org [Internet]. APPA; 2018 [cited 2018]. Available: https://americanpetproducts.org/pubs_survey.asp

4. Masters AM, McGreevy PD. Dogkeeping practices as reported by readers of an Australian dog enthusiast magazine. AUST VET J. Wiley Online Library; 2008;86: 18–25. doi: 10.1111/j.1751-0813.2007.00248.x 18271818

5. Beaver BV. Owner complaints about canine behavior. J AM VET MED ASSOC. 1994;204: 1953–1955. 8077144

6. Hart BL, Eckstein RA. The role of gonadal hormones in the occurrence of objectionable behaviours in dogs and cats. APPL ANIM BEHAV SCI. Elsevier; 1997;52: 331–344.

7. Lockwood R. The ethology and epidemiology of canine aggression. The domestic dog: its evolution, behaviour, and interactions with people. Cambridge, New York: Cambridge University Press; 2017. pp. 160–181.

8. Roll A, Unshelm J. Aggressive conflicts amongst dogs and factors affecting them. APPL ANIM BEHAV SCI. 1997;52: 229–242.

9. Line S, Voith V. Dominance aggression of dogs towards people: behavior profile and response to treatment. Applied Animal Behaviour Science. 1986;16: 77–83.

10. Svartberg K, Forkman B. Personality traits in the domestic dog (Canis familiaris). APPL ANIM BEHAV SCI. 2002;79: 133–155.

11. Starling MJ, Branson N, Thomson PC, McGreevy PD. Age, sex and reproductive status affect boldness in dogs. VET J. Elsevier Ltd; 2013;197: 868–872. doi: 10.1016/j.tvjl.2013.05.019 23778256

12. Blackshaw JK. An overview of types of aggressive behaviour in dogs and methods of treatment. APPL ANIM BEHAV SCI. Elsevier; 1991;30: 351–361.

13. Podberscek AL, Serpell JA. Environmental influences on the expression of aggressive behaviour in English Cocker Spaniels. APPL ANIM BEHAV SCI. Elsevier; 1997;52: 215–227.

14. Guy NC, Luescher UA, Dohoo SE, Spangler E, Miller JB, Dohoo IR, et al. A case series of biting dogs: characteristics of the dogs, their behaviour, and their victims. APPL ANIM BEHAV SCI. Elsevier; 2001;74: 43–57.

15. Spain CV, Scarlett JM, Houpt KA. Long-term risks and benefits of early-age gonadectomy in dogs. Journal of the American Veterinary Medical Association. 2004;224: 380–387. doi: 10.2460/javma.2004.224.380 14765797

16. Reisner IR, Houpt KA, Shofer FS. National survey of owner-directed aggression in English Springer Spaniels. Journal of the American Veterinary Medical Association. 2005;227: 1594–1603. doi: 10.2460/javma.2005.227.1594 16313036

17. Maarschalkerweerd RJ, Endenburg N, Kirpensteijn J, Knol BW. Influence of orchiectomy on canine behaviour. VET REC. 2nd ed. 1997;140: 617–619. doi: 10.1136/vr.140.24.617 9228691

18. McGreevy P, Masters A. Risk factors for separation-related distress and feed-related aggression in dogs: Additional findings from a survey of Australian dog owners. APPL ANIM BEHAV SCI. 2008;109: 320–328. doi: 10.1016/j.applanim.2007.04.001

19. Trevejo R, Yang M, Lund EM. Epidemiology of surgical castration of dogs and cats in the United States. J AM VET MED ASSOC. 2011;238: 898–904. doi: 10.2460/javma.238.7.898 21453178

20. O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC. Prevalence of Disorders Recorded in Dogs Attending Primary-Care Veterinary Practices in England. Rosenfeld CS, editor. PLOS ONE. 2014;9: e90501–16. doi: 10.1371/journal.pone.0090501 24594665

21. Sánchez-Vizcaíno F, Noble P-JM, Jones PH, Menacere T, Buchan I, Reynolds S, et al. Demographics of dogs, cats, and rabbits attending veterinary practices in Great Britain as recorded in their electronic health records. BMC VET RES. BioMed Central; 2017;13: 218. doi: 10.1186/s12917-017-1138-9 28693574

22. Kubinyi E, Turcsán B, Miklósi Á. Dog and owner demographic characteristics and dog personality trait associations. BEHAV PROCESS. 2009;81: 392–401. doi: 10.1016/j.beproc.2009.04.004 19520239

23. Howe LM. Current perspectives on the optimal age to spay/castrate dogs and cats. Veterinary medicine (Auckland, NZ). 2015 ed. 2015;6: 171–180. doi: 10.2147/vmrr.S53264 30101104

24. Farhoody P, Mallawaarachchi I, Tarwater PM, Serpell JA, Duffy DL, Zink C. Aggression toward Familiar People, Strangers, and Conspecifics in Gonadectomized and Intact Dogs. FRONT VET SCI. 2018;5: 239–13. doi: 10.3389/fvets.2018.00239

25. Root Kustritz MV. Pros, Cons, and Techniques of Pediatric Neutering. VET CLIN NORTH AM SMALL ANIM PRACT. 2014;44: 221–233. doi: 10.1016/j.cvsm.2013.10.002 24580988

26. Marston LC, Bennett PC, Coleman GJ. What Happens to Shelter Dogs? An Analysis of Data for 1 Year From Three Australian Shelters. J APPL ANIM WELF SCI. 2004;7: 27–47. doi: 10.1207/s15327604jaws0701_2 15066769

27. Patronek GJ, Glickman LT, Moyer MR. Population dynamics and the risk of euthanasia for dogs in an animal shelter. ANTHROZOOS. 1995;8: 31–43.

28. Kass PH, New JC Jr., Scarlett JM, Salman MD. Understanding Animal Companion Surplus in the United States: Relinquishment of Nonadoptables to Animal Shelters for Euthanasia. J APPL ANIM WELF SCI. 2001;4: 237–248. doi: 10.1207/S15327604JAWS0404_01

29. McGreevy PD, Bennett PC. Challenges and paradoxes in the companion-animal niche. ANIMAL WELFARE-POTTERS BAR. Universities Federation for Animal Welfare; 2010;19: 11–16.

30. Boyd C, Jarvis S, McGreevy P, Heath S, Church D, Brodbelt D, et al. Mortality resulting from undesirable behaviours in dogs aged under three years attending primary-care veterinary practices in England. Animal Welfare. 2018;27: 251–262. doi: 10.7120/09627286.27.3.251

31. McGreevy PD, Wilson B, Starling MJ, Serpell JA. Behavioural risks in male dogs with minimal lifetime exposure to gonadal hormones may complicate population-control benefits of desexing. Yildirim A, editor. PLOS ONE. 2018;13: e0196284–14. doi: 10.1371/journal.pone.0196284 29718954

32. O’Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC. Longevity and mortality of owned dogs in England. VET J. Elsevier Ltd; 2013;198: 638–643. doi: 10.1016/j.tvjl.2013.09.020 24206631

33. Smith A. The Role of Neutering in Cancer Development. VET CLIN NORTH AM SMALL ANIM PRACT. 2014;44: 965–975. doi: 10.1016/j.cvsm.2014.06.003 25174910

34. Reichler IM. Gonadectomy in Cats and Dogs: A Review of Risks and Benefits. REPROD DOMEST ANIM. Wiley/Blackwell (10.1111); 2009;44: 29–35. doi: 10.1111/j.1439-0531.2009.01437.x 19754532

35. Hagman R. Canine pyometra: What is new? REPROD DOMEST ANIM. 2017;52: 288–292. doi: 10.1111/rda.12843 27807901

36. Jitpean S, Hagman R, Ström Holst B, Höglund OV, Pettersson A, Egenvall A. Breed Variations in the Incidence of Pyometra and Mammary Tumours in Swedish Dogs. REPROD DOMEST ANIM. Wiley/Blackwell (10.1111); 2012;47: 347–350. doi: 10.1111/rda.12103 23279535

37. Hagman R. Pyometra in Small Animals. VET CLIN NORTH AM SMALL ANIM PRACT. 2018;48: 639–661. doi: 10.1016/j.cvsm.2018.03.001 29933767

38. Egenvall A, Hagman R, Bonnett BN, Hedhammar Å, Olson P, Lagerstedt A-S. Breed Risk of Pyometra in Insured Dogs in Sweden. J VET INTERN MED. Wiley/Blackwell (10.1111); 2001;15: 530–538. doi: 10.1892/0891-6640(2001)015<0530:bropii>2.3.co;2 11817057

39. Gibson A, Dean R, Yates D, Stavisky J. A retrospective study of pyometra at five RSPCA hospitals in the UK: 1728 cases from 2006 to 2011. VET REC. 2013;173: 396. doi: 10.1136/vr.101514 24114733

40. Kyles AE, Douglass JP, Rottman JB. Pyelonephritis following inadvertent excision of the ureter during ovariohysterectomy in a bitch. VET REC. 1996;139: 471–472. doi: 10.1136/vr.139.19.471 8938968

41. Dow C. The Cystic Hyperplasia-Pyometra Complex in the Bitch. Journal of Comparative Pathology and Therapeutics. Elsevier; 1959;69: 237–IN18. doi: 10.1016/S0368-1742(59)80023-0

42. van Goethem B, SCHAEFERS-OKKENS A, KIRPENSTEIJN J. Making a rational choice between ovariectomy and ovariohysterectomy in the dog: a discussion of the benefits of either technique. Veterinary Surgery. 2006;35: 136–143. doi: 10.1111/j.1532-950X.2006.00124.x 16472293

43. Schneider R, Dorn CR, Taylor DO. Factors influencing canine mammary cancer development and postsurgical survival. J NATL CANCER INST. 1969;43: 1249–1261. 4319248

44. Beauvais W, Cardwell JM, Brodbelt DC. The effect of neutering on the risk of mammary tumours in dogs—a systematic review. J SMALL ANIM PRACT. 2012;53: 314–322. doi: 10.1111/j.1748-5827.2011.01220.x 22647210

45. Byron JK, Taylor KH, Phillips GS, Stahl MS. Urethral Sphincter Mechanism Incompetence in 163 Neutered Female Dogs: Diagnosis, Treatment, and Relationship of Weight and Age at Neuter to Development of Disease. J VET INTERN MED. Hoboken: John Wiley and Sons Inc; 2017;31: 442–448. doi: 10.1111/jvim.14678 28256023

46. Johnston SD, Root Kustritz MV, Olson PNS. Canine and Feline Theriogenology. Canine and Feline Theriogenology. Philadelphia: WB Saunders; 2001. pp. 80–87.

47. Root Kustritz MV. Effects of Surgical Sterilization on Canine and Feline Health and on Society. REPROD DOMEST ANIM. Wiley/Blackwell (10.1111); 2012;47: 214–222. doi: 10.1111/j.1439-0531.2012.02078.x 22827373

48. Root AL, Parkin TD, Hutchison P, Warnes C, Yam PS. Canine pseudopregnancy: an evaluation of prevalence and current treatment protocols in the UK. BMC VET RES. 2018;14: 170. doi: 10.1186/s12917-018-1493-1 29793494

49. McKenzie R. Species and hybrid cultivars ofLilium(lilies). In: McKenzie R, editor. Australia's Poisonous Plants, Fungi and Cyanobacteria. CSIRO Publishing; 2012.

50. Jitpean S, Ström-Holst B, Emanuelson U, Höglund OV, Pettersson A, Alneryd-Bull C, et al. Outcome of pyometra in female dogs and predictors of peritonitis and prolonged postoperative hospitalization in surgically treated cases. BMC VET RES. BioMed Central; 2014;10: 6–6. doi: 10.1186/1746-6148-10-6 24393406

51. Hart BL, Hart LA, Thigpen AP, Willits NH. Neutering of German Shepherd Dogs: associated joint disorders, cancers and urinary incontinence. Veterinary Medicine and Science. Wiley-Blackwell; 2016;2: 191–199. doi: 10.1002/vms3.34 29067194

52. Kent MS, Burton JH, Dank G, Bannasch DL, Rebhun RB. Association of cancer-related mortality, age and gonadectomy in golden retriever dogs at a veterinary academic center (1989–2016). PLOS ONE. Public Library of Science; 2018;13: e0192578. doi: 10.1371/journal.pone.0192578 29408871

53. Adin CA. Complications of Ovariohysterectomy and Orchiectomy in Companion Animals. VET CLIN NORTH AM SMALL ANIM PRACT. 2011;41: 1023–1039. doi: 10.1016/j.cvsm.2011.05.004 21889699

54. DeLay J. Perianesthetic Mortality in Domestic Animals: A Retrospective Study of Postmortem Lesions and Review of Autopsy Procedures. VET PATHOL. SAGE Publications Inc; 2016;53: 1078–1086. doi: 10.1177/0300985816655853 27371539

55. Levy JK, Bard KM, Tucker SJ, Diskant PD, Dingman PA. Perioperative mortality in cats and dogs undergoing spay or castration at a high-volume clinic. VET J. British Association of Oral and Maxillofacial Surgeons; 2017;224: 11–15. doi: 10.1016/j.tvjl.2017.05.013 28697869

56. Hagman R. Pyometra in Small Animals. VET CLIN NORTH AM SMALL ANIM PRACT. 2018;48: 639–661. doi: 10.1016/j.cvsm.2018.03.001 29933767

57. O’Neill DG, Riddell A, Church DB, Owen L, Brodbelt DC, Hall JL. Urinary incontinence in bitches under primary veterinary care in England: prevalence and risk factors. J SMALL ANIM PRACT. Wiley/Blackwell (10.1111); 2017;58: 685–693. doi: 10.1111/jsap.12731 28881018

58. Seguin MA, Vaden SL, Altier C, Stone E, Levine JF. Persistent Urinary Tract Infections and Reinfections in 100 Dogs (1989–1999). J VET INTERN MED. Wiley/Blackwell (10.1111); 2008;17: 622–631. doi: 10.1111/j.1939-1676.2003.tb02492.x 14529127

59. Kustritz MV. Determining the optimal age for gonadectomy of dogs and cats. Journal of the American Veterinary Medical Association. 2007 ed. 2007;231: 1665–1675. doi: 10.2460/javma.231.11.1665 18052800

60. Belanger JM, Bellumori TP, Bannasch DL, Famula TR, Oberbauer AM. Correlation of neuter status and expression of heritable disorders. CANINE GENET EPIDEMIOL. 2017;4: 6. doi: 10.1186/s40575-017-0044-6 28560045

61. Torres de la Riva G, Hart BL, Farver TB, Oberbauer AM, Messam LLM, Willits N, et al. Neutering Dogs: Effects on Joint Disorders and Cancers in Golden Retrievers. PLOS ONE. Public Library of Science; 2013;8: e55937. doi: 10.1371/journal.pone.0055937 23418479

62. Bermingham EN, Thomas DG, Cave NJ, Morris PJ, Butterwick RF, German AJ. Energy Requirements of Adult Dogs: A Meta-Analysis. PLOS ONE. Public Library of Science; 2014;9: e109681. doi: 10.1371/journal.pone.0109681 25313818

63. Overall KL, Beebe AD. Dominance aggression in young female dogs: what does this suggest about the heterogeneity of the disorder. 1997.

64. Bálint A, Rieger G, Miklósi Á, Pongrácz P. Assessment of owner-directed aggressive behavioural tendencies of dogs in situations of possession and manipulation. R SOC OPEN SCI. 2017;4.

65. Kim HH, Yeon SC, Houpt KA, Lee HC, Chang HH, Lee HJ. Effects of ovariohysterectomy on reactivity in German Shepherd dogs. VET J. 2006;172: 154–159. doi: 10.1016/j.tvjl.2005.02.028 16772140

66. Balogh O, Borruat N, Andrea Meier A, Hartnack S, Reichler IM. The influence of spaying and its timing relative to the onset of puberty on urinary and general behaviour in Labrador Retrievers. REPROD DOMEST ANIM. Wiley/Blackwell (10.1111); 2018;53: 1184–1190. doi: 10.1111/rda.13225 29974985

67. McKenzie B. Evaluating the benefits and risks of neutering dogs and cats. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. Wallingford: CABI; 2010;5: 1–18. doi: 10.1079/PAVSNNR20105045

68. Heidenberger E, Unshelm J. Changes in the behavior of dogs after castration. TIERÄRZTLICHE. 1990;18: 69–75.

69. McGreevy PD. A Modern Dog's Life. Randwick, NSW: UNSW Press; 2009.

70. O'Farrell V, Peachey E. Behavioural effects of ovariohysterectomy on bitches. J SMALL ANIM PRACT. 1990;31: 595–598.

71. Takeuchi Y, Houpt K, Scarlett J. Evaluation of treatments for separation anxiety in dogs. J AM VET MED ASSOC. 2000;217: 342–345. doi: 10.2460/javma.2000.217.342 10935036

72. Mongillo P, Scandurra A, D’Aniello B, Marinelli L. Effect of sex and gonadectomy on dogs’ spatial performance. APPL ANIM BEHAV SCI. Elsevier B.V; 2017;191: 84–89. doi: 10.1016/j.applanim.2017.01.017

73. Scandurra A, Marinelli L, Lõoke M, D’Aniello B, Mongillo P. The effect of age, sex and gonadectomy on dogs’ use of spatial navigation strategies. APPL ANIM BEHAV SCI. Elsevier; 2018;205: 89–97. doi: 10.1016/j.applanim.2018.05.010

74. Scandurra A, Alterisio A, Di Cosmo A, D’Ambrosio A, D’Aniello B. Ovariectomy Impairs Socio-Cognitive Functions in Dogs. ANIMALS. 2019;9: 58–7. doi: 10.3390/ani9020058 30769794

75. Hsu Y, Serpell JA. Development and validation of a questionnaire for measuring behavior and temperament traits in pet dogs. Journal of the American Veterinary Medical Association. 2003;223: 1293–1300. doi: 10.2460/javma.2003.223.1293 14621216

76. Duffy DL, Serpell JA. Predictive validity of a method for evaluating temperament in young guide and service dogs. APPL ANIM BEHAV SCI. Elsevier B.V; 2012;138: 99–109. doi: 10.1016/j.applanim.2012.02.011

77. Agresti A. Categorical Data Analysis. Hoboken, New Jersey: John Wiley & Sons, Inc; pp. 282–284.

78. McGreevy P, Thomson P, Dhand N, Raubenheimer D, Masters S, Mansfield C, et al. VetCompass Australia: A National Big Data Collection System for Veterinary Science. ANIMALS. 2017;7: 74–15. doi: 10.3390/ani7100074 28954419

79. McGreevy P, Starling M, Payne E, Bennett P. Defining and measuring dogmanship: A new multidisciplinary science to improve understanding of human–dog interactions. VET J. Elsevier Ltd; 2017;229: 1–5. doi: 10.1016/j.tvjl.2017.10.015 29183567

80. Appleby D, Pluijmakers J. Separation anxiety in dogs: the function of homeostasis in its development and treatment. CLINTECH SMALL AN P. 2004;19: 205–215.

81. Cannas S, Frank D, Minero M, Aspesi A, Benedetti R, Palestrini C. Journal of Veterinary Behavior. J VET BEHAV. Elsevier Ltd; 2014;9: 50–57. doi: 10.1016/j.jveb.2013.12.002

82. Storengen LM, Boge SCK, Strøm SJ, Løberg G, Lingaas F. A descriptive study of 215 dogs diagnosed with separation anxiety. APPL ANIM BEHAV SCI. Elsevier B.V; 2014;159: 82–89. doi: 10.1016/j.applanim.2014.07.006

83. Scaglia E, Cannas S, Minero M, Frank D, Bassi A, Palestrini C. Journal of Veterinary Behavior. J VET BEHAV. Elsevier Ltd; 2013;: 1–6. doi: 10.1016/j.jveb.2013.04.065

84. Taylor AM, Ratcliffe VF, McComb K, Reby D. Auditory communication in domestic dogs: Vocal signalling in the extended social environment of a companion animal. In: Kaminski J, Marshall-Pescini S, editors. The Social Dog. 1st ed. Oxford, UK; 2014.

85. Wells D. A review of environmental enrichment for kennelled dogs, Canis familiaris. APPL ANIM BEHAV SCI. 2004;85: 307–317.

86. Rooney N, Gaines S, Hiby E. A practitioner's guide to working dog welfare. J VET BEHAV. Elsevier Inc; 2009;4: 127–134. doi: 10.1016/j.jveb.2008.10.037

87. Hubrecht R. A comparison of social and environmental enrichment methods for laboratory housed dogs. Applied Animal Behaviour Science. 1993;37: 345–361. doi: 10.1016/0168-1591(93)90123-7

88. De Meester R, De Bacquer D, Peremans K. A preliminary study on the use of the Socially Acceptable Behavior test as a test for …. J VET BEHAV. 2008.

89. van der Borg JAM, Beerda B, Ooms M, De Souza AS, Van Hagen M, Kemp B. Evaluation of behaviour testing for human directed aggression in dogs. APPL ANIM BEHAV SCI. Elsevier B.V; 2010;128: 78–90. doi: 10.1016/j.applanim.2010.09.016

90. De Meester RH, De Bacquer D, Peremans K, Vermeire S, Planta DJ, Coopman F, et al. A preliminary study on the use of the Socially Acceptable Behavior test as a test for shyness/confidence in the temperament of dogs. J VET BEHAV. 2008;3: 161–170. doi: 10.1016/j.jveb.2007.10.005

91. Konok V, Kosztolányi A, Rainer W, Mutschler B, Halsband U, Miklósi Á. Influence of Owners’ Attachment Style and Personality on Their Dogs’ (Canis familiaris) Separation-Related Disorder. PLOS ONE.; 2015;10: e0118375. doi: 10.1371/journal.pone.0118375 25706147

92. Cockcroft PD, Holmes MA. Handbook of Evidence-based Veterinary Medicine. Oxford: Wiley Blackwell; 2003.

93. Palmer C, Corr S, Sandoe P. Inconvenient desires: should we routinely neuter companion animals? Anthrozoos. 2012;25: S153–S172.

94. Wolfe TM. The Great Spay/Neuter Debate: Does Early Gonadectomy Prevent or Cause Disease in Dogs? ADV SMALL ANIM MED SURG. 2015;28: 1–3. doi: 10.1016/j.asams.2015.12.001


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