Analysis of gut microbiota of obese individuals with type 2 diabetes and healthy individuals

Autoři: Aftab Ahmad aff001;  Wanwei Yang aff002;  Guofang Chen aff002;  Muhammad Shafiq aff001;  Sundus Javed aff001;  Syed Shujaat Ali Zaidi aff001;  Ramla Shahid aff001;  Chao Liu aff002;  Habib Bokhari aff001
Působiště autorů: Department of Biosciences, COMSATS University, Chak Shahzad, Islamabad, Pakistan aff001;  Endocrine and Diabetes Center, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, China aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0226372


Type 2 diabetes mellitus (T2DM) accounts for 90% of diabetes cases worldwide. The majority of T2DM patients are obese. Dysbiosis in the gut microflora is strongly associated with the pathogenesis of obesity and T2DM; however, the microbiome of obese-T2DM individuals in the Pakistani population remains unexplored. The gut microbiota signature of 60 Pakistani adults was studied using 16S rRNA sequencing targeting V3–V4 hypervariable regions. The sequence analysis revealed that bacteria from Firmicutes were predominant along with those from Clostridia and Negativicutes, whereas bacteria from Verrucomicrobia, Bacteroidetes, Proteobacteria, and Elusimicrobia were less abundant among the obese T2DM patients. These data distinctively vary from those in reports on the Indian population. The difference in gut microbiota could presumably be related to the distinct lifestyle and eastern dietary habits (high carbohydrate and fat intake, low fiber intake) and unregulated antibiotic consumption. This is the first study carried out to understand the gut microbiome and its correlation with individual life style of obese T2DM patients in the Pakistani population.

Klíčová slova:

Clostridium – Gut bacteria – Microbiome – Obesity – Sequence databases – Species diversity


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2019 Číslo 12