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preLekára.sk / Odborné časopisy / PLOS One / 2020 - 1

The role of demographic history and selection in shaping genetic diversity of the Galápagos penguin (Spheniscus mendiculus)

English version

Autoři: Gabriella Arauco-Shapiro aff001;  Katelyn I. Schumacher aff001;  Dee Boersma aff002;  Juan L. Bouzat aff001
Působiště autorů: Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America aff001;  Center for Ecosystem Sentinels and Department of Biology, University of Washington, Seattle, Washington, United States of America aff002
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0226439

Souhrn

Although many studies have documented the effects of demographic bottlenecks on the genetic diversity of natural populations, there is conflicting evidence of the roles that genetic drift and selection may play in driving changes in genetic variation at adaptive loci. We analyzed genetic variation at microsatellite and mitochondrial loci in conjunction with an adaptive MHC class II locus in the Galápagos penguin (Spheniscus mendiculus), a species that has undergone serial demographic bottlenecks associated with El Niño events through its evolutionary history. We compared levels of variation in the Galápagos penguin to those of its congener, the Magellanic penguin (Spheniscus magellanicus), which has consistently maintained a large population size and thus was used as a non-bottlenecked control. The comparison of neutral and adaptive markers in these two demographically distinct species allowed assessment of the potential role of balancing selection in maintaining levels of MHC variation during bottleneck events. Our analysis suggests that the lack of genetic diversity at both neutral and adaptive loci in the Galápagos penguin likely resulted from its restricted range, relatively low abundance, and history of demographic bottlenecks. The Galápagos penguin revealed two MHC alleles, one mitochondrial haplotype, and six alleles across five microsatellite loci, which represents only a small fraction of the diversity detected in Magellanic penguins. Despite the decreased genetic diversity in the Galápagos penguin, results revealed signals of balancing selection at the MHC, which suggest that selection can mitigate some of the effects of genetic drift during bottleneck events. Although Galápagos penguin populations have persisted for a long time, increased frequency of El Niño events due to global climate change, as well as the low diversity exhibited at immunological loci, may put this species at further risk of extinction.

Klíčová slova:

Haplotypes – Genetic loci – Natural selection – Alleles – Population genetics – Species diversity – Genetic drift – Penguins

Zdroje

1. Frankham R, Ballou JD, Briscoe DA. Introduction to Conservation Genetics. 1st ed. Cambridge: Cambridge University Press; 2002.

2. Bouzat JL. Conservation genetics of population bottlenecks: the role of chance, selection, and history. Conserv Genet. 2010; 11 : 463–478.

3. Wright S. Evolution in Mendelian populations. Genetics. 1931; 16 : 97–159. 17246615

4. Nei M, Maruyama T, Chakraborty R. The bottleneck effect and genetic variability in populations. Evolution. 1975; 29 : 1–10. doi: 10.1111/j.1558-5646.1975.tb00807.x 28563291

5. Frankham R. Relationship of genetic variation to population size in wildlife. Conserv Biol. 1996; 10 : 1500–1508.

6. Gitzendanner MA, Soltis PS. Patterns of genetic variation in rare and widespread plant congeners. Am J Bot. 2000; 87 : 783–792. 10860909

7. Reed DH. Albatrosses, eagles, and newts, Oh My: exceptions to the prevailing paradigm concerning genetic diversity and population viability? Anim Conserv. 2010; 13 : 448–457.

8. O’Brien SJ, Johnson WE, Driscoll CA, Dobrynin P, Marker L. Conservation genetics of the cheetah: lessons learned and new opportunities. J Hered 2017; 671–677. doi: 10.1093/jhered/esx047 28821181

9. Hughes AL, Nei M. Nucleotide substitution at major histocompatibility complex class II loci: evidence for overdominant selection. Proc Natl Acad Sci USA. 1989; 86 : 958–962. doi: 10.1073/pnas.86.3.958 2492668

10. Accolla RS, Adorini L, Sartoris S, Sinigaglia F, Guardiola J. MHC: Orchestrating the immune response. Immunol Today. 1995; 16 : 8–11. doi: 10.1016/0167-5699(95)80063-8 7880390

11. Bollmer JL, Vargas FH, Parker PG. Low MHC variation in the endangered Galápagos penguin (Spheniscus mendiculus). Immunogenetics. 2007; 59 : 593–602. doi: 10.1007/s00251-007-0221-y 17457582

12. Radwan J, Biedrzycka A, Babik W. Does reduced MHC diversity decrease viability of vertebrate populations? Biol Conserv. 2010; 143 : 537–544.

13. Alcaide M, Edwards SV, Negro JJ, Serrano D, Tella JL. Extensive polymorphism and geographical variation at a positively selected MHC class II B gene of the lesser kestrel (Falco naumanni). Mol Ecol. 2008; 17 : 2652–2665. doi: 10.1111/j.1365-294X.2008.03791.x 18489548

14. Hughes CR, Miles S, Walbroehl JM. Support for the minimal essential MHC hypothesis: A parrot with a single, highly polymorphic MHC class II B gene. Immunogenetics. 2008; 60 : 219–231. doi: 10.1007/s00251-008-0287-1 18431567

15. Potts WK, Wakeland EK. Evolution of MHC genetic diversity: a tale of incest, pestilence and sexual preference. Trends Genet. 1993; 9 : 408–412. doi: 10.1016/0168-9525(93)90103-o 8122307

16. Eimes JA, Bollmer JL, Whittingham LA, Johnson JA, Van Oosterhout C, Dunn PO. Rapid loss of MHC class II variation in a bottlenecked population is explained by drift and loss of copy number variation. J Evol Biol. 2011; 24 : 1847–1856. doi: 10.1111/j.1420-9101.2011.02311.x 21605219

17. Sutton JT, Nakagawa S, Robertson BC, Jamieson IG. Disentangling the roles of natural selection and genetic drift in shaping variation at MHC immunity genes. Mol Ecol. 2011; 20 : 4408–4420. doi: 10.1111/j.1365-294X.2011.05292.x 21981032

18. Spurgin LG, Richardson DS. How pathogens drive genetic diversity: MHC, mechanisms and misunderstandings. Proc R Soc Lond B. 2010; 277 : 979–988.

19. Doherty PC, Zinkernagel RM. Enhanced immunological surveillance in mice heterozygous at the H-2 gene complex. Nature. 1975; 256 : 50–52. doi: 10.1038/256050a0 1079575

20. Hughes AL, Nei M. Pattern of nucleotide substitution at major histocompatibility complex class I loci reveals overdominant selection. Nature. 1988; 335 : 167–170. doi: 10.1038/335167a0 3412472

21. Clarke BC, Kirby DRS. Maintenance of histocompatibility polymorphism. Nature. 1966; 211 : 999–1000. doi: 10.1038/211999a0 6007869

22. Slade RW, McCallum H. Overdominant vs. frequency-dependent selection at MHC loci. Genetics. 1992; 132 : 861–862. 1468635

23. Hill AVS. HLA associations with malaria in Africa: some implications for MHC evolution. In: Klein J, Klein D, editors. Molecular Evolution of the Major Histocompatibility Complex. NATO ASI Series (Series H: Cell Biology) vol 59. Berlin: Springer; 1991. pp 403–420.

24. Hedrick PW, Thomson G. Evidence for balancing selection at HLA. Genetics. 1983; 104 : 449–456. 6884768

25. Miller HC, Lambert DM. Genetic drift outweighs balancing selection in shaping post-bottleneck major histocompatibility complex variation in New Zealand robins (Petroicidae). Mol Ecol. 2004; 13 : 3709–3721. doi: 10.1111/j.1365-294X.2004.02368.x 15548285

26. Campos JL, Posada D, Morán P. Genetic variation at MHC, mitochondrial and microsatellite loci in isolated populations of Brown trout (Salmo trutta). Conserv Genet. 2006; 7 : 515–530.

27. Radwan J, Kawalko A, Wójcik JM, Babik W. MHC-DRB3 variation in a free-living population of the European bison, Bison bonasus. Mol Ecol. 2007; 16 : 531–540. doi: 10.1111/j.1365-294X.2006.03179.x 17257111

28. Babik W, Pabijan M, Radwan J. Contrasting patterns of variation in MHC loci in the Alpine newt. Mol Ecol. 2008; 17 : 2339–2355. doi: 10.1111/j.1365-294X.2008.03757.x 18422929

29. Maruyama T, Nei M. Genetic variability maintained by mutation and overdominant selection in finite populations. Genetics. 1984; 98 : 441–459.

30. Ejsmond MJ, Radwan J. MHC diversity in bottlenecked populations: A simulation model. Conserv Genet. 2011; 12 : 129–137.

31. Elena SF, Cooper VS, Lenski RE. Punctuated evolution caused by selection of rare beneficial mutations. Science. 1996; 272 : 1802–1804. doi: 10.1126/science.272.5269.1802 8650581

32. Satta Y, O’hUigin C, Takahata N, Klein J. Intensity of natural selection at the major histocompatibility complex loci. Proc Natl Acad Sci USA. 1994; 91 : 7184–7188. doi: 10.1073/pnas.91.15.7184 8041766

33. Bos DH, Gopurenko D, Williams RN, DeWoody JA. Inferring population history and demography using microsatellites, mitochondrial DNA, and major histocompatibility complex (MHC) genes. Evolution. 2008; 62 : 1458–1468. doi: 10.1111/j.1558-5646.2008.00364.x 18331461

34. Seddon JM, Baverstock PR. Variation on islands: major histocompatibility complex (Mhc) polymorphism in populations of the Australian bush rat. Mol Ecol. 1999; 8 : 2071–2079. doi: 10.1046/j.1365-294x.1999.00822.x 10632858

35. Hedrick PW, Gutiérrez-Espeleta GA, Lee RN. Founder effect in an island population of bighorn sheep. Mol Ecol. 2001; 10 : 851–857. doi: 10.1046/j.1365-294x.2001.01243.x 11348494

36. Aguilar A, Roemer G, Debenham S, Binns M, Garcelon D, Wayne RK. High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal. Proc Natl Acad Sci USA. 2004; 101 : 3490–3494. doi: 10.1073/pnas.0306582101 14990802

37. Van Oosterhout C, Joyce DA, Cummings SM, Blais J, Barson NJ, Ramnarine IW, et al. Balancing selection, random genetic drift, and genetic variation at the major histocompatibility complex in two wild populations of guppies (Poecilia reticulata). Evolution. 2006; 60 : 2562–2574. 17263117

38. Mona S, Crestanella B, Bankhead-Dronnet S, Pecchioli E, Ingrosso S, D’Amelio S, et al. Disentangling the effects of recombination, selection, and demography on the genetic variation at a major histocompatibility complex class II gene in the alpine chamois. Mol Ecol. 2008; 17 : 4053–4067. doi: 10.1111/j.1365-294x.2008.03892.x 19238706

39. Oliver MK, Piertney SB. Selection maintains MHC diversity through a natural population bottleneck. Mol Biol Evol. 2012; 29 : 1713–1720. doi: 10.1093/molbev/mss063 22323362

40. Schuster AC, Herde A, Mazzoni CJ, Eccard JA, Sommer S. Evidence for selection maintaining MHC diversity in a rodent species despite strong density fluctuations. Immunogenetics. 2016; 68 : 429–437. doi: 10.1007/s00251-016-0916-z 27225422

41. Zhai T, Yang H-Q, Zhang R-C, Fang L-M, Zhong G-H, Fang S-G. Effects of population bottleneck and balancing selection on the Chinese alligator are revealed by locus-specific characterization of MHC genes. Sci Rep. 2017; 7 : 5549. doi: 10.1038/s41598-017-05640-2 28717152

42. Hedrick PW, Kalinowski ST. Inbreeding depression in conservation biology. Annu Rev Ecol Syst. 2000; 31 : 139–262.

43. Frankham R, Gilligan DM, Morris D, Briscoe DA. Inbreeding and extinction: effects of purging. Conserv Genet. 2001; 2 : 279–285.

44. Radwan J. Inbreeding depression in fecundity and inbred line extinction in the bulb mite, Rhizoglyphus robini. Heredity. 2003; 371–376. doi: 10.1038/sj.hdy.6800254 12714982

45. Swindell WR, Bouzat JL. Ancestral inbreeding reduces the magnitude of inbreeding depression in Drosophila melanogaster. Evolution. 2006; 60 : 762–767. 16739457

46. Swindell WR, Bouzat JL. Reduced inbreeding depression due to historical inbreeding in Drosophila melanogaster: evidence for purging. J Evol Biol. 2006; 19 : 1257–1264. doi: 10.1111/j.1420-9101.2005.01074.x 16780526

47. Boersma PD. Breeding patterns of Galapagos penguins as an indicator of oceanographic conditions. Science. 1978; 200 : 1481–1483. doi: 10.1126/science.200.4349.1481 17757690

48. Vargas H, Lougheed C, Snell H. Population size and trends of the Galápagos Penguin Spheniscus mendiculus. Ibis. 2005; 147 : 367–374.

49. Boersma PD, Frere E, Kane O, Pozzi LM, Pütz K, Raya Rey A, et al. Magellanic penguins. In: García Borboroglu P, Boersma PD, editors. Penguins: Natural History and Conservation. Seattle: University of Washington Press; 2013. pp 233–263.

50. Boersma PD. An ecological and behavioral study of the Galápagos penguin. Living Bird. 1976; 15 : 43–93.

51. Valle CA. Status of the Galápagos penguin and flightless cormorant populations in 1985. Noticias de Galápagos. 1986; 43 : 16–17.

52. Valle CA, Coulter MC. Present status of the flightless cormorant, Galápagos penguin and greater flamingo populations in the Galápagos Islands, Ecuador, after the 1982–83 El Niño. Condor. 1987; 89 : 276–281.

53. Vargas FH, Harrison S, Rea S, Macdonald DW. Biological effects of El Niño on the Galápagos penguin. Biol Conserv. 2006; 127 : 107–114.

54. Boersma PD. Population trends of the Galápagos penguin: impacts on El Niño and La Niña. Condor. 1998; 100 : 245–253.

55. Vargas FH, Lacy RC, Johnson PJ, Macdonald D. Modelling the effect of El Niño on the persistence of small populations: the Galápagos penguin as a case study. Biol Conserv. 2007; 137 : 138–148.

56. Boersma PD, Cappello CD, Merlen G. First observations of post-fledging care in Galapagos penguins (Spheniscus mendiculus). Wilson J of Ornithol. 2017; 129 : 186–191.

57. Pütz K, Schiavini A, Rey AR, Lüthi BH. Winter migration of Magellanic penguins (Spheniscus magellanicus) from the southernmost distributional range. Mar Biol. 2007; 152 : 1227–1235.

58. Stokes DL, Boersma PD, Lopez de Casenave J, García-Borboroglu P. Conservation of migratory Magellanic penguins requires marine zoning. Biol Conserv. 2014; 170 : 151–161.

59. Rebstock GA, Boersma PD, García-Borboroglu P. Changes in habitat use and nesting density in a declining seabird colony. Popul Ecol. 2016; 58 : 105–119.

60. Dantas GPM, Maria GC, Marasco ACM, Castro LT, Almeida VS, Santos FR, et al. Demographic history of the Magellanic penguin (Spheniscus magellanicus) on the Pacific and Atlantic coasts of South America. J Ornithol. 2018; 159 : 643–655.

61. Gandini P, Frere E, Boersma PD. Status and conservation of Magellanic penguins Spheniscus magellanicus in Patagonia, Argentina. Bird Conserv Int. 1996; 6 : 307–316.

62. Pozzi LM, García Borboroglu P, Boersma PD, Pascual MA. Population regulation in Magellanic penguins: what determines changes in colony size? PLoS One. 2015; 10: e0119002. doi: 10.1371/journal.pone.0119002 25786254

63. Kimura M. The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press; 1983.

64. Bouzat JL, Lewin HA, Paige KN. The ghost of genetic diversity past: historical DNA analysis of the Greater Prairie Chicken. Am Nat. 1998; 152 : 1–6. doi: 10.1086/286145 18811397

65. Akst EP, Boersma PD, Fleischer RC. A comparison of genetic diversity between the Galápagos penguin and the Magellanic penguin. Conserv Genet. 2002; 3 : 375–383.

66. Frankham R. Conservation genetics. Annu Rev Genet. 1995; 29 : 305–327. doi: 10.1146/annurev.ge.29.120195.001513 8825477

67. Frankham R, Lees K, Montgomery ME, England PR, Lowe EH, Briscoe DA. Do population size bottlenecks reduce evolutionary potential? Anim Conserv. 1999; 2 : 255–260.

68. Vrijenhoek RC. Genetic diversity and fitness in small populations. In: Loeschcke V, Jain SK, Tomiuk J, editors. Conservation Genetics. EXS, vol 68. Basel: Birkhäuser; 1994. pp 37–53.

69. Saccheri I, Kuussaari M, Kankare M, Vikman P, Fortelius W, Hanski I. Inbreeding and extinction in a butterfly metapopulation. Nature. 1998; 392 : 491–494.

70. Frankham R. Genetics and extinction. Biol Conserv. 2005; 126 : 131–140.

71. Paterson S, Wilson K, Pemberton JM. Major histocompatibility complex variation associated with juvenile survival and parasite resistance in a large unmanaged ungulate population (Ovis aries L.). Proc Natl Acad Sci USA. 1998; 95 : 3714–3719. doi: 10.1073/pnas.95.7.3714 9520432

72. Seutin G, White BN, Boag TP. Preservation of avian blood and tissue samples for DNA analysis. Can J Zool. 1991; 69 : 82–90.

73. Frere E, Gandini P. Distribución reproductiva y abundancia de las aves marinas de Santa Cruz. Parte 2: de Bahía Laura a Punta Dungeness. In: Yorio P, Frere E, Gandini P, Harris G., editors. Atlas de la distribución reproductiva de aves marinas en el litoral Patagónico Argentino. Buenos Aires: Society Instituto Salesiano de Artes Gráficas; 1998. pp 153–177.

74. Bouzat JL, Lyons AC, Knafler GJ, Boersma PD. Environmental determinants of genetic structure in Magellanic Penguin breeding colonies of the Atlantic and Pacific Oceans. Poster session presented at the VIII International Penguin Conference; 2013 Sep 2–6; Bristol, United Kingdom.

75. Bouzat JL, Walker BG, Boersma PD. Regional genetic structure in the Magellanic penguin (Spheniscus magellanicus) suggests metapopulation dynamics. Auk. 2009; 126 : 326–334.

76. Nims BD, Vargas FH, Merkel J, Parker PG. Low genetic diversity and lack of population structure in the endangered Galápagos penguin (Spheniscus mendiculus). Conserv Genet. 2008; 9 : 1413–1420.

77. BirdLife International and Handbook of the Birds of the World. Bird species distribution maps of the world, version 2018.1. Available at: http://datazone.birdlife.org/species/requestdis.

78. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1989.

79. Kikkawa EF, Tsuda TT, Naruse TK, Sumiyama D, Fukuda M, Kurita M, et al. Analysis of the sequence variations in the Mhc DRB1-like gene of the endangered Humboldt penguin (Spheniscus humboldti). Immunogenetics. 2005; 57 : 99–107. doi: 10.1007/s00251-005-0774-6 15714307

80. Kikkawa EF, Tsuda TT, Sumiyama D, Naruse TK, Fukuda M, Kurita M, et al. Trans-species polymorphism of the Mhc class II DRB-like gene in banded penguins (genus Spheniscus). Immunogenetics. 2009; 61 : 341–352. doi: 10.1007/s00251-009-0363-1 19319519

81. Knafler GJ, Clark JA, Boersma PD, Bouzat JL. MHC diversity and mate choice in the Magellanic penguin, Spheniscus magellanicus. J Hered. 2012; 103 : 759–768. doi: 10.1093/jhered/ess054 22952272

82. Bollmer JL, Dunn PO, Whittingham LA, Wimpee C. Extensive MHC class II B gene duplication in a passerine, the common yellowthroat (Geothlypis trichas). J Hered. 2010; 101 : 448–460. doi: 10.1093/jhered/esq018 20200139

83. Davies CJ, Andersson L, Ellis SA, Hensen EJ, Lewin HA, Mikko S, et al. Nomenclature for the factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee. Anim Genet. 1997; 28 : 159–168.

84. Kennedy LJ, Altet L, Angles JM, Barnes A, Carter SD, Francino O, et al. Nomenclature for the factors of the dog major histocompatibility system (DLA), 1998: first report of the ISAG DLA Nomenclature Committee. Tissue Antigens. 1999; 54 : 312–321. doi: 10.1034/j.1399-0039.1999.540319.x 10519375

85. Marsh SG, Bodmer JG, Albert ED, Bodmer WF, Bontrop RE, Dupont B, et al. Nomenclature for factors of the HLA system, 2000. Tissue Antigens. 2001; 57 : 236–283. doi: 10.1034/j.1399-0039.2001.057003236.x 11285132

86. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, et al. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012; 28 : 1647–1649. doi: 10.1093/bioinformatics/bts199 22543367

87. Wright S. The genetical structure of populations. Ann Eugen. 1949; 15 : 323–354.

88. Raymond M, Rousset F. GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered. 1995; 86 : 248–249.

89. Rousset F. Genepop'007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour. 2008; 8 : 103–106. doi: 10.1111/j.1471-8286.2007.01931.x 21585727

90. Rozas J, Sánchez-DelBarrio JC, Messeguer X, Rozas R. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003; 19 : 2496–2497. doi: 10.1093/bioinformatics/btg359 14668244

91. Excoffier L, Lischer HEL. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour. 2010; 10 : 564–567. doi: 10.1111/j.1755-0998.2010.02847.x 21565059

92. Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989; 123 : 585–595. 2513255

93. Ewens WJ. The sampling theory of selectively neutral alleles. Theor Popul Biol. 1972; 3 : 87–112. doi: 10.1016/0040-5809(72)90035-4 4667078

94. Watterson G. The homozygosity test of neutrality. Genetics. 1978; 88 : 405–417. 17248803

95. Slatkin M. An exact test for neutrality based on the Ewens sampling distribution. Genet Res. 1994; 68 : 71–74.

96. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016; 33 : 1870–1874. doi: 10.1093/molbev/msw054 27004904

97. Nei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986; 3 : 418–426. doi: 10.1093/oxfordjournals.molbev.a040410 3444411

98. Brown JH, Jardetzky TS, Gorga JC, Stern LJ, Urban RG, Strominger JL, et al. Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature. 1993; 364 : 33–39. doi: 10.1038/364033a0 8316295

99. Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol Evol. 2015; 6 : 1110–1116.

100. Clement M, Posada D, Crandall KA. TCS: a computer program to estimate gene genealogies. Mol Ecol. 2000; 9 : 1657–1659. doi: 10.1046/j.1365-294x.2000.01020.x 11050560

101. Slatkin M. A measure of population subdivision based on microsatellite allele frequencies. Genetics. 1995; 139 : 457–462. 7705646

102. Cornuet JM, Luikart G. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics. 1996; 144 : 2001–2014. 8978083

103. Piry S, Luikart G, Cornuet JM. BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. J Hered. 1999; 90 : 502–503.

104. Kimura M, Ohta T. Stepwise mutation model and distribution of allelic frequencies in a finite population. Proc Natl Acad Sci USA. 1978; 75 : 2868–2872. doi: 10.1073/pnas.75.6.2868 275857

105. DiRienzo A, Peterson AC, Garza JC, Valdes AM, Slatkin M, Freimer NB. Mutational processes of simple-sequence repeat loci in human populations. Proc Natl Acad Sci USA. 1994; 91 : 3166–3170. doi: 10.1073/pnas.91.8.3166 8159720

106. Luikart G, Allendorf FW, Cornuet JM, Sherwin WB. Distortion of allele frequency distributions provides a test for recent population bottlenecks. J Hered. 1998; 89 : 238–247. doi: 10.1093/jhered/89.3.238 9656466

107. Simonsen KL, Churchill GA, Aquadro CF. Properties of statistical tests of neutrality for DNA polymorphism data. Genetics. 1995; 141 : 413–429. 8536987

108. Maruyama T, Fuerst PA. Population bottlenecks and nonequilibrium models in population genetics. II. Number of alleles in a small population that was formed by a recent bottleneck. Genetics. 1985; 111 : 675–689. 4054612

109. Garrigan D, Hedrick PW. Perspective: detecting adaptive molecular polymorphism: lessons from the MHC. Evolution. 2003; 57 : 1707–1722. doi: 10.1111/j.0014-3820.2003.tb00580.x 14503614

110. Miller HC, Miller KA, Daugherty CH. Reduced MHC variation in a threatened tuatara species. Anim Conserv. 2008; 11 : 206–214.

111. Hedrick PW, Lee RN, Garrigan D. Major histocompatibility complex variation in red wolves: evidence for common ancestry with coyotes and balancing selection. Mol Ecol. 2002; 11 : 1905–1913. doi: 10.1046/j.1365-294x.2002.01579.x 12296935

112. Ellegren H, Hartman G, Johansson M, Andersson L. Major histocompatibility complex monomorphism and low levels of DNA fingerprinting variability in a reintroduced and rapidly expanding population of beavers. Proc Natl Acad Sci USA. 1993; 90 : 8150–8153. doi: 10.1073/pnas.90.17.8150 8367476

113. Hedrick PW, Parker KM, Lee RN. Using microsatellite and MHC variation to identify species, ESUs, and MUs in the endangered Sonoran topminnow. Mol Ecol. 2001; 10 : 1399–1412. doi: 10.1046/j.1365-294x.2001.01289.x 11412363

114. Weber DS, Stewart BS, Schienman J, Lehman N. Major histocompatibility complex variation at three class II loci in the northern elephant seal. Mol Ecol. 2004; 13 : 711–718. doi: 10.1111/j.1365-294x.2004.02095.x 14871373

115. O’Brien SJ, Evermann JF. Interactive influence of infectious disease and genetic diversity in natural populations. Trends Ecol Evol. 1988; 3 : 254–259. doi: 10.1016/0169-5347(88)90058-4 21227241

116. Slade RW. Limited MHC polymorphism in the southern elephant seal: implications for MHC evolution and marine mammal population biology. Proc R Soc Lond B. 1992; 249 : 163–171.

117. Hedrick PW, Parker KM, Gutiérrez-Espeleta GA, Rattink A, Lievers K. Major histocompatibility complex variation in the Arabian oryx. Evolution. 2000; 5 : 2145–2151.

118. Levin II, Outlaw DC, Vargas FH, Parker PG. Plasmodium blood parasite found in endangered Galápagos penguins (Spheniscus mendiculus). Biol Conserv. 2009; 142 : 3191–3195.

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Článek Recognition of personality disorder and anxiety disorder comorbidity in patients treated for depression in secondary psychiatric care
Článek Correction: Strategies for achieving high sequencing accuracy for low diversity samples and avoiding sample bleeding using illumina platform
Článek ETAPOD: A forecast model for prediction of black pod disease outbreak in Nigeria
Článek Deliver on Your Own: Disrespectful Maternity Care in rural Kenya
Článek Number of days required to estimate physical activity constructs objectively measured in different age groups: Findings from three Brazilian (Pelotas) population-based birth cohorts
Článek Exploring the mechanism of olfactory recognition in the initial stage by modeling the emission spectrum of electron transfer
Článek Risk of complications among diabetics self-reporting oral health status in Canada: A population-based cohort study
Článek Practical considerations in the use of a porcine model (Sus scrofa domesticus) to assess prevention of postoperative peritubal adhesions
Článek Identifying maintenance hosts for infection with Dichelobacter nodosus in free-ranging wild ruminants in Switzerland: A prevalence study
Článek Production, purification and evaluation of biodegradation potential of PHB depolymerase of Stenotrophomonas sp. RZS7
Článek The impact of a wireless audio system on communication in robotic-assisted laparoscopic surgery: A prospective controlled trial
Článek Seroprevalence of viral and vector-borne bacterial pathogens in domestic dogs (Canis familiaris) in northern Botswana
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Článek Post-transcriptional regulation of Rad51c by miR-222 contributes cellular transformation
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Článek Prognostics for pain in osteoarthritis: Do clinical measures predict pain after total joint replacement?
Článek Effects of Transcranial Direct Current Stimulation on GABA and Glx in Children: A pilot study
Článek Brief communication: Long-term absence of Langerhans cells alters the gene expression profile of keratinocytes and dendritic epidermal T cells
Článek Morphological diversity within a core collection of subterranean clover (Trifolium subterraneum L.): Lessons in pasture adaptation from the wild
Článek Feasibility of real-time in vivo 89Zr-DFO-labeled CAR T-cell trafficking using PET imaging
Článek Repository-based plasmid design
Článek A new method of recording from the giant fiber of Drosophila melanogaster shows that the strength of its auditory inputs remains constant with age
Článek Aberrant cervical innate immunity predicts onset of dysbiosis and sexually transmitted infections in women of reproductive age
Článek Safe mobility, socioeconomic inequalities, and aging: A 12-year multilevel interrupted time-series analysis of road traffic death rates in a Latin American country
Článek Lesion of striatal patches disrupts habitual behaviors and increases behavioral variability
Článek A clinical method for estimating the modulus of elasticity of the human cornea in vivo
Článek Patient perceived value of teleophthalmology in an urban, low income US population with diabetes
Článek Evidence in support of chromosomal sex influencing plasma based metabolome vs APOE genotype influencing brain metabolome profile in humanized APOE male and female mice
Článek Accelerated sparsity based reconstruction of compressively sensed multichannel EEG signals
Článek Dense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cells
Článek Gamma Knife radiosurgery for vestibular schwannomas: Evaluation of planning using the sphericity degree of the target volume
Článek Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)
Článek A study to better understand under-utilization of laboratory tests for antenatal care in Senegal
Článek Physicians’ perspectives regarding non-medical switching of prescription medications: Results of an internet e-survey
Článek Effectiveness of information technology–enabled ‘SMART Eating’ health promotion intervention: A cluster randomized controlled trial
Článek Cauda Equina Syndrome Core Outcome Set (CESCOS): An international patient and healthcare professional consensus for research studies
Článek A new species of Macrocypraea (Gastropoda, Cypraeidae) from Trindade Island, Brazil, including phenotypic differentiation from remaining congeneric species
Článek Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit
Článek A new approach to the temporal significance of house orientations in European Early Neolithic settlements
Článek Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
Článek Effect of classroom intervention on student food selection and plate waste: Evidence from a randomized control trial
Článek Preventing HIV and HSV-2 through knowledge and attitudes: A replication study of a multi-component community-based intervention in Zimbabwe
Článek A randomized trial of a behavioral intervention to decrease hospital length of stay by decreasing bedrest
Článek Color image segmentation using adaptive hierarchical-histogram thresholding
Článek Potential socioeconomic impacts from ocean acidification and climate change effects on Atlantic Canadian fisheries
Článek Female finches prefer courtship signals indicating male vigor and neuromuscular ability
Článek The effect of spatial position and age within an egg-clutch on embryonic development and key metabolic enzymes in two clownfish species, Amphiprion ocellaris and Amphiprion frenatus
Článek The impact of translated reminder letters and phone calls on mammography screening booking rates: Two randomised controlled trials
Článek Application of a genetic algorithm to the keyboard layout problem
Článek Design and evaluation of a laboratory-based wheelchair castor testing protocol using community data
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Článek A Comprehensive Data Gathering Network Architecture in Large-Scale Visual Sensor Networks
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Článek Modeling aggressive market order placements with Hawkes factor models
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Článek High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer
Článek Complex patterns of cell growth in the placenta in normal pregnancy and as adaptations to maternal diet restriction
Článek Tofu intake is inversely associated with risk of breast cancer: A meta-analysis of observational studies
Článek Temporal ordering of input modulates connectivity formation in a developmental neuronal network model of the cortex
Článek Healthy lifestyle index and its association with hypertension among community adults in Sri Lanka: A cross-sectional study
Článek From organ to cell: Multi-level telomere length assessment in patients with idiopathic pulmonary fibrosis
Článek How do critical care staff respond to organisational challenge? A qualitative exploration into personality types and cognitive processing in critical care
Článek An investigation of transportation practices in an Ontario swine system using descriptive network analysis
Článek Comparison of gridded precipitation datasets for rainfall-runoff and inundation modeling in the Mekong River Basin
Článek The effects of dual-task cognitive interference on gait and turning in Huntington’s disease
Článek Effects of Allium hookeri on gut microbiome related to growth performance in young broiler chickens
Článek Novel imaging biomarkers for mapping the impact of mild mitochondrial uncoupling in the outer retina in vivo
Článek Hyperkalemia treatment modalities: A descriptive observational study focused on medication and healthcare resource utilization
Článek Comparison of post-transplantation diabetes mellitus incidence and risk factors between kidney and liver transplantation patients
Článek A definition-by-example approach and visual language for activity patterns in engineering disciplines
Článek A network analysis revealed the essential and common downstream proteins related to inguinal hernia
Článek Use of conventional cardiac troponin assay for diagnosis of non-ST-elevation myocardial infarction: ‘The Ottawa Troponin Pathway’
Článek Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells
Článek Research on motion planning for an indoor spray arm based on an improved potential field method
Článek Detailed analysis of the transverse arch of hallux valgus feet with and without pain using weightbearing ultrasound imaging and precise force sensors
Článek Surrogate R-spondins for tissue-specific potentiation of Wnt Signaling
Článek Apolipoprotein-AI mimetic peptides D-4F and L-5F decrease hepatic inflammation and increase insulin sensitivity in C57BL/6 mice
Článek Treating patients with driving phobia by virtual reality exposure therapy – a pilot study
Článek Efficient processing of raster and vector data
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Článek Diagnostic differences in respiratory breathing patterns and work of breathing indices in children with Duchenne muscular dystrophy
Článek Proportions of CD4 test results indicating advanced HIV disease remain consistently high at primary health care facilities across four high HIV burden countries
Článek Single-modal and multi-modal false arrhythmia alarm reduction using attention-based convolutional and recurrent neural networks
Článek Eye-gaze information input based on pupillary response to visual stimulus with luminance modulation
Článek Post mortem evaluation of inflammation, oxidative stress, and PPARγ activation in a nonhuman primate model of cardiac sympathetic neurodegeneration
Článek Were ancient foxes far more carnivorous than recent ones?—Carnassial morphological evidence
Článek Disruption in daily eating-fasting and activity-rest cycles in Indian adolescents attending school
Článek Are changes in depressive symptoms, general health and residential area socio-economic status associated with trajectories of waist circumference and body mass index?
Článek Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
Článek Tobacco smoking and health-related quality of life among university students: Mediating effect of depression
Článek The Shapley value for a fair division of group discounts for coordinating cooling loads
Článek Incidence of hospital-acquired pressure ulcers in patients with "minimal risk" according to the "Norton-MI" scale
Článek Lipoprotein(a) plasma levels are not associated with survival after acute coronary syndromes: An observational cohort study
Článek Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine
Článek Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice
Článek Biochemical characterization of Ty1 retrotransposon protease
Článek The validation of the Beijing version of the Montreal Cognitive Assessment in Chinese patients undergoing hemodialysis
Článek Inflammasome expression is higher in ovarian tumors than in normal ovary
Článek Adherence to iron-folic acid supplement and associated factors among antenatal care attending pregnant mothers in governmental health institutions of Adwa town, Tigray, Ethiopia: Cross-sectional study
Článek Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae
Článek Preclinical evaluation of AT-527, a novel guanosine nucleotide prodrug with potent, pan-genotypic activity against hepatitis C virus
Článek Clinical, cytogenetic and molecular genetic characterization of a tandem fusion translocation in a male Holstein cattle with congenital hypospadias and a ventricular septal defect
Článek Detection of Torque Teno Virus (TTV) and TTV-Like Minivirus in patients with presumed infectious endophthalmitis in India
Článek Estimating the basic reproduction number of a pathogen in a single host when only a single founder successfully infects
Článek What drugs modify the risk of iatrogenic impulse-control disorders in Parkinson’s disease? A preliminary pharmacoepidemiologic study
Článek Community- and trophic-level responses of soil nematodes to removal of a non-native tree at different stages of invasion
Článek Association of ECG parameters with late gadolinium enhancement and outcome in patients with clinical suspicion of acute or subacute myocarditis referred for CMR imaging
Článek Catchment-scale export of antibiotic resistance genes and bacteria from an agricultural watershed in central Iowa
Článek Impact of multi-drug resistant bacteria on economic and clinical outcomes of healthcare-associated infections in adults: Systematic review and meta-analysis
Článek Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay
Článek Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers
Článek Intraoperative computed tomography imaging for dose calculation in intraoperative electron radiation therapy: Initial clinical observations
Článek Simple non-mydriatic retinal photography is feasible and demonstrates retinal microvascular dilation in Chronic Obstructive Pulmonary Disease (COPD)
Článek Maternal depressive symptoms and children’s cognitive development: Does early childcare and child’s sex matter?
Článek Evaluation of a bioengineered ACL matrix’s osteointegration with BMP-2 supplementation
Článek Psychosocial profiles of physical activity fluctuation in office employees: A latent profile analysis
Článek Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada
Článek Soluble AXL as a marker of disease progression and survival in melanoma
Článek Using machine learning methods to determine a typology of patients with HIV-HCV infection to be treated with antivirals
Článek Gender differences influence over insomnia in Korean population: A cross-sectional study
Článek Impact of scion/rootstock reciprocal effects on metabolomics of fruit juice and phloem sap in grafted Citrus reticulata
Článek Adapting cognitive diagnosis computerized adaptive testing item selection rules to traditional item response theory
Článek Umbilical cord separation time, predictors and healing complications in newborns with dry care
Článek Analysis of attitudinal components towards statistics among students from different academic degrees
Článek Effects of fatigue induced by repeated-sprint on kicking accuracy and velocity in female soccer players
Článek A pre-clinical validation plan to evaluate analytical sensitivities of molecular diagnostics such as BD MAX MDR-TB, Xpert MTB/Rif Ultra and FluoroType MTB
Článek Clinical correlates associated with the long-term response of bipolar disorder patients to lithium, valproate or lamotrigine: A retrospective study
Článek On the genus Crossaster (Echinodermata: Asteroidea) and its distribution
Článek An integrated vitamin E-coated polymer hybrid nanoplatform: A lucrative option for an enhanced in vitro macrophage retention for an anti-hepatitis B therapeutic prospect
Článek The effect of strontium and silicon substituted hydroxyapatite electrochemical coatings on bone ingrowth and osseointegration of selective laser sintered porous metal implants
Článek Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma species in dogs with hemangiosarcoma from across the United States
Článek Color discrimination and gas chromatography-mass spectrometry fingerprint based on chemometrics analysis for the quality evaluation of Schizonepetae Spica
Článek Comparisons of recurrence-free survival and overall survival between microwave versus radiofrequency ablation treatment for hepatocellular carcinoma: A multiple centers retrospective cohort study with propensity score matching
Článek Oral misoprostol, low dose vaginal misoprostol, and vaginal dinoprostone for labor induction: Randomized controlled trial
Článek The association between dietary patterns before and in early pregnancy and the risk of gestational diabetes mellitus (GDM): Data from the Malaysian SECOST cohort
Článek Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution
Článek Prediction of Uropathogens by Flow Cytometry and Dip-stick Test Results of Urine Through Multivariable Logistic Regression Analysis
Článek Interleukin 6 is increased in preclinical HNSCC models of acquired cetuximab resistance, but is not required for maintenance of resistance
Článek Impact of viral disease hypophagia on pig jejunal function and integrity
Článek Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia
Článek cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection
Článek Spectral characteristics of urine from patients with end-stage kidney disease analyzed using Raman Chemometric Urinalysis (Rametrix)
Článek Fast quantitative time lapse displacement imaging of endothelial cell invasion
Článek Two novel mutations in MSX1 causing oligodontia
Článek Dome-shaped macula in children and adolescents
Článek Targeted transcriptomic study of the implication of central metabolic pathways in mannosylerythritol lipids biosynthesis in Pseudozyma antarctica T-34
Článek Preliminary evidences of the presence of extracellular DNA single stranded forms in soil
Článek Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis
Článek Morphological association between the muscles and bones in the craniofacial region
Článek Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection
Článek Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum
Článek Antenatal depression and its association with adverse birth outcomes in low and middle-income countries: A systematic review and meta-analysis
Článek The role of refugee and migrant migration status on medication adherence: Mediation through illness perceptions
Článek Effects of CK2β subunit down-regulation on Akt signalling in HK-2 renal cells
Článek Novel broad-spectrum activity-based probes to profile malarial cysteine proteases
Článek Association between opioid analgesic therapy and initiation of buprenorphine management: An analysis of prescription drug monitoring program data
Článek Effect of a community-based approach of iron and folic acid supplementation on compliance by pregnant women in Kiambu County, Kenya: A quasi-experimental study
Článek An updated evaluation of serum sHER2, CA15.3, and CEA levels as biomarkers for the response of patients with metastatic breast cancer to trastuzumab-based therapies
Článek Plasma kynurenines and prognosis in patients with heart failure
Článek Occurrence and distribution of anthropogenic persistent organic pollutants in coastal sediments and mud shrimps from the wetland of central Taiwan
Článek Gut microbiota composition alterations are associated with the onset of diabetes in kidney transplant recipients
Článek Metabolic analysis of amino acids and vitamin B6 pathways in lymphoma survivors with cancer related chronic fatigue
Článek Immunopathogenesis of canine chronic ulcerative stomatitis
Článek Generalizing findings from a randomized controlled trial to a real-world study of the iLookOut, an online education program to improve early childhood care and education providers’ knowledge and attitudes about reporting child maltreatment
Článek When and what to test for: A cost-effectiveness analysis of febrile illness test-and-treat strategies in the era of responsible antibiotic use
Článek Comparison of effects and safety in providing controlled hypotension during surgery between dexmedetomidine and magnesium sulphate: A meta-analysis of randomized controlled trials
Článek The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
Článek Association of endothelial nitric oxide synthase (NOS3) gene polymorphisms with primary open-angle glaucoma in a Saudi cohort
Článek Antitrust analysis with upward pricing pressure and cost efficiencies
Článek Natural selection contributes to food web stability
Článek Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum
Článek Sofosbuvir-based regimen for genotype 2 HCV infected patients in Taiwan: A real world experience
Článek The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function
Článek Quantitative analysis of adsorption and desorption of volatile organic compounds on reusable zeolite filters using gas chromatography
Článek Quo vadis Pantanal? Expected precipitation extremes and drought dynamics from changing sea surface temperature
Článek Cloud-computing and machine learning in support of country-level land cover and ecosystem extent mapping in Liberia and Gabon
Článek The Brief Measure of Emotional Preoperative Stress (B-MEPS) as a new predictive tool for postoperative pain: A prospective observational cohort study
Článek The impact of diabetes mellitus medication on the incidence of endogenous endophthalmitis
Článek Correction: Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition
Článek Clinical and pathological features of thrombotic microangiopathy influencing long-term kidney transplant outcomes
Článek Occupational exposure to particulate matter from air pollution in the outdoor workplaces in Almaty during the cold season
Článek Key necroptotic proteins are required for Smac mimetic-mediated sensitization of cholangiocarcinoma cells to TNF-α and chemotherapeutic gemcitabine-induced necroptosis
Článek Concurrent lipidomics and proteomics on malignant plasma cells from multiple myeloma patients: Probing the lipid metabolome
Článek Association between tuberculosis and depression on negative outcomes of tuberculosis treatment: A systematic review and meta-analysis
Článek Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds
Článek Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates
Článek Oral magnesium supplementation for leg cramps in pregnancy—An observational controlled trial
Článek Health care professionals’ knowledge of commonly used sedative, analgesic and neuromuscular drugs: A single center (Rambam Health Care Campus), prospective, observational survey
Článek Campylobacter portucalensis sp. nov., a new species of Campylobacter isolated from the preputial mucosa of bulls
Článek Transgenic interleukin 11 expression causes cross-tissue fibro-inflammation and an inflammatory bowel phenotype in mice
Článek Sleep quality and sex modify the relationships between trait energy and fatigue on state energy and fatigue
Článek The role of peer, parental, and school norms in predicting adolescents’ attitudes and behaviours of majority and different minority ethnic groups in Croatia
Článek Availability, prices and affordability of selected antibiotics and medicines against non-communicable diseases in western Cameroon and northeast DR Congo
Článek The effect of mutations derived from mouse-adapted H3N2 seasonal influenza A virus to pathogenicity and host adaptation
Článek Detection of posttraumatic pneumothorax using electrical impedance tomography—An observer-blinded study in pigs with blunt chest trauma
Článek Educators’ perceptions of organisational readiness for implementation of a pre-adolescent transdisciplinary school health intervention for inter-generational outcomes
Článek Beyond the heterodimer model for mineralocorticoid and glucocorticoid receptor interactions in nuclei and at DNA
Článek Framework and algorithms for identifying honest blocks in blockchain
Článek Exploring the impact of terminology differences in blood and organ donor decision making
Článek Platelet indices significantly correlate with liver fibrosis in HCV-infected patients
Článek The nitrate content of fresh and cooked vegetables and their health-related risks
Článek Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results
Článek Non-gradient and genotype-dependent patterns of RSV gene expression
Článek Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants
Článek The 24-hour urinary cortisol in post-traumatic stress disorder: A meta-analysis
Článek Drug-eluting versus bare-metal stents for first myocardial infarction in patients with atrial fibrillation: A nationwide population-based cohort study
Článek Health-related quality of life among patients with type 2 diabetes mellitus in Eastern Province, Saudi Arabia: A cross-sectional study
Článek “I like the way I am, but I feel like I could get a little bit bigger”: Perceptions of body image among adolescents and youth living with HIV in Durban, South Africa
Článek Nanoparticle-based ‘turn-on’ scattering and post-sample fluorescence for ultrasensitive detection of water pollution in wider window
Článek Insights into the strategy of micro-environmental adaptation: Transcriptomic analysis of two alvinocaridid shrimps at a hydrothermal vent
Článek Thirty-day readmission after medical-surgical hospitalization for people who experience imprisonment in Ontario, Canada: A retrospective cohort study
Článek Hyper-spectral response and estimation model of soil degradation in Kenli County, the Yellow River Delta
Článek The association of telomere length and telomerase activity with adverse outcomes in older patients with non-ST-elevation acute coronary syndrome
Článek Construction of a high-density genetic map and fine mapping of a candidate gene locus for a novel branched-spike mutant in barley
Článek Alterations of aqueous humor Aβ levels in Aβ-infused and transgenic mouse models of Alzheimer disease
Článek Natural hybridization between Phyllagathis and Sporoxeia species produces a hybrid without reproductive organs
Článek The impact of peer attachment on prosocial behavior, emotional difficulties and conduct problems in adolescence: The mediating role of empathy
Článek Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia
Článek Characterization of black patina from the Tiber River embankments using Next-Generation Sequencing
Článek Problem gambling, associations with comorbid health conditions, substance use, and behavioural addictions: Opportunities for pathways to treatment
Článek Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution
Článek Validity of cerebrovascular ICD-9-CM codes in healthcare administrative databases. The Umbria Data-Value Project
Článek Torque teno virus viral load is related to age, CMV infection and HLA type but not to Alzheimer's disease
Článek Associations of cigarette smoking and burden of thoracic aortic calcification in asymptomatic individuals: A dose-response relationship
Článek Transforming assessment of speech in children with cleft palate via online crowdsourcing
Článek Human-raptor conflict in rural settlements of Colombia
Článek Assessment of peritoneal microbial features and tumor marker levels as potential diagnostic tools for ovarian cancer
Článek Deficiency syndromes in top predators associated with large-scale changes in the Baltic Sea ecosystem
Článek Perceived relative social status and cognitive load influence acceptance of unfair offers in the Ultimatum Game
Článek Hepatitis B and C virus infection among HIV patients within the public and private healthcare systems in Chile: A cross-sectional serosurvey
Článek Retraction: Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis
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PLOS One

2020 Číslo 1
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Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
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