Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system


Autoři: Yuichi Furuhata aff001;  Ayako Sakai aff001;  Tomi Murakami aff001;  Akira Nagasaki aff001;  Yoshio Kato aff001
Působiště autorů: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan aff001
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0227477

Souhrn

Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin–luciferase system should be effective in plant biology. However, the suitable optical window for a luminescence system in plants remains unexplored. In this study, we sought to determine the optical window and optimal luciferase reporter system for terrestrial plant analyses using Arabidopsis thaliana as a model organism. We compared six different luciferase systems and found the green enhanced Nano-lantern (GeNL)–furimazine combination to be the optimal luciferase reporter. Spectral measurements of GeNL–furimazine showed that its luminescence peak falls within the range of optical transparency for chlorophyll and, therefore, enables greater penetration through a layer of cultured A. thaliana cells. Moreover, A. thaliana plants expressing GeNL with furimazine emitted strong luminescence, which could be detected even with the naked eye. Thus, the GeNL–furimazine combination should facilitate biological analyses of genes and cellular functions in A. thaliana and all other terrestrial plants.

Klíčová slova:

Agrobacteria – Arabidopsis thaliana – Cell cultures – Fluorescence – Chlorophyll – Luciferase – Luminescence – Plant cells


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2020 Číslo 1

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