preLekára.sk / Odborné časopisy / PLOS One / 2020 - 1

Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit

English version

Autoři: A. M. Abdelatty aff001;  O. A. M. Badr aff002;  S. A. Mohamed aff002;  M. S. Khattab aff003;  SH. M. Dessouki aff004;  O. A. A. Farid aff005;  A. A. Elolimy aff006;  O. G. Sakr aff004;  M. A. Elhady aff009;  G. Mehesen aff004;  M. Bionaz aff010
Působiště autorů: Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff001;  Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt aff002;  Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff003;  Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt aff004;  Department of Physiology, National Organization for Drug Control and Research, Giza, Egypt aff005;  Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America aff006;  Arkansas Children’s Nutrition Center, Little Rock, AR, United States of America aff007;  Department of Animal Production, National Research Centre, Giza, Egypt aff008;  Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff009;  Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR, United States of America aff010
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pone.0226070

Souhrn

Conjugated linoleic acid (CLA) is known for its multiple benefits including improvement of growth, increasing lean mass, and anti-carcinogenic effects. However, when used in long-term supplementations CLA does not improve semen parameters in boar and bull and reduces fertility in Japanese quails. The content of unsaturated fatty acids in dietary lipids plays a significant role in spermatogenesis owning the high proportion of unsaturated fatty acids in plasma membrane of sperms. Whether CLA plays a role in testicular tissue and epididymal fat is still unknown. Therefore, in this study we hypothesize that long-term supplementation of equal proportion of CLA isomer mix (c9,t11-CLA and t10,c12- CLA) in rabbit bucks might alter male reproductive potentials. Twelve V-Line weaned male rabbits were used in 26 weeks trial, rabbits were individually raised and randomly allocated into three dietary groups. Control group (CON) received a basal diet, a group received 0.5% CLA (CLA 0.5%), and a group received 1% CLA (CLA 1%). Rabbits were euthanized at the end of the trial and several parameters were evaluated related to growth, semen quality, and testicular and epididymal tissue histopathology and transcriptome. The long-term supplementation of CLA increased feed intake by 5% and body weight by 2–3%. CLA 1% decreased sperm progressive motility. In testicular tissue L-carnitine and α-tocopherol were decreased by CLA supplementation. In epididymal fat, CLA tended to decrease concentration of polyunsaturated fatty acids, the expression of SCD5 gene was upregulated by CLA 1% and CASP3 gene was upregulated by CLA 0.5%. Transcription of PPARG was downregulated by CLA. Feeding 1% CLA also decreased testicular epithelial thickness. Long-term supplementation of CLA modestly enhanced male rabbit growth, but negatively impacted male reproduction, especially at high dose of CLA.

Klíčová slova:

Body weight – Fatty acids – Diet – Fats – Rabbits – Sperm – Hematoxylin staining – Semen

Zdroje

1. Chen PB and Park Y. Conjugated Linoleic Acid in Human Health: Effects on Weight Control. In Nutrition in the Prevention and Treatment of Abdominal Obesity. Academic Press. 2019; 355–382.

2. Mitchell PL and McLeod RS. Conjugated linoleic acid and atherosclerosis: studies in animal models. Biochemistry and Cell Biology. 2008; 86 (4), pp. 293–301. doi: 10.1139/o08-070 18756324

3. Viladomiu M, Hontecillas R, and Bassaganya-Riera, J. Modulation of inflammation and immunity by dietary conjugated linoleic acid. European Journal of Pharmacology. 2016; 785, pp. 87–95. doi: 10.1016/j.ejphar.2015.03.095 25987426

4. Wood JD. Meat composition and nutritional value. In Lawrie´s Meat Science. 2017; Jan 1 (pp. 635–659). Woodhead Publishing.

5. Matin S, Nemati A, Ghobadi H, Alipanah-Moghadam R, and Rezagholizadeh L. The effect of conjugated linoleic acid on oxidative stress and matrix metalloproteinases 2 and 9 in patients with COPD. International Journal of Chronic Obstructive Pulmonary Disease. 2018; 13: p. 1449–1459. doi: 10.2147/COPD.S155985 29765212

6. Onakpoya IJ, Posadzki PP, Watson LK, Davies LA, and Ernst E. The efficacy of long-term conjugated linoleic acid (CLA) supplementation on body composition in overweight and obese individuals: a systematic review and meta-analysis of randomized clinical trials. European Journal of Nutrition. 2012; 51(2), pp. 127–134. doi: 10.1007/s00394-011-0253-9 21990002

7. Karimi R, Towhidi A, Zeinoaldini S, Rezayazdi K, Mousavi M, Safari H et al. Effects of supplemental conjugated linoleic acids (CLA) on fresh and post‐thaw sperm quality of Holstein bulls. Reproduction in Domestic Animals. 2017; 52(3), pp. 459–467. doi: 10.1111/rda.12932 28176378

8. Teixeira SMP, Chaveiro AEN, and da Silva JFM. Effect of trans-10, cis-12 isomer of conjugated linoleic acid on boar semen quality after cryopreservation. Animal Reproduction. 2017; 14(2), pp. 400–405.

9. De Veth MJ, Bauman DE, Koch W, Mann GE, Pfeiffer AM and Butler WR. Efficacy of conjugated linoleic acid for improving reproduction: A multi-study analysis in early-lactation dairy cows. Journal of Dairy Science. 2009; 92(6), pp. 2662–2669. doi: 10.3168/jds.2008-1845 19447999

10. Zamora-Zamora V, Figueroa-Velasco JL, Cordero-Mora JL, Nieto-Aquino R, García-Contreras ADC, Sánchez-Torres MT. Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile. Veterinaria México. 2017; 4(3), pp. 1–15.

11. Khodaei H, Chamani M, Sadeghi A, and Hejazi H. Effects of Conjugated Linoleic Acid (CLA) on hormones and factors involved in murine ovulation. Journal of Reproduction & Infertility. 2009; 10(2), pp. 101–108.

12. Aydin R, Karaman M, Toprak HHC, Ozugur AK, Aydin D, and Cicek T. The effect of long-term feeding of conjugated linoleic acid on fertility in Japanese quail. South African Journal of Animal Science. 2006; 36(2), pp. 99–104.

13. Ali TM. A manual for the primary animal health care worker. Food and Agriculture Organization (FAO). 1994.

14. Gliozzi TM, Zaniboni L, Maldjian A, Luzi F, Maertens L, and Cerolini S. Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets. Theriogenology. 2009; 71(6), pp. 910–919. doi: 10.1016/j.theriogenology.2008.10.022 19121864

15. National Research Council. Nutrient Requirements of Rabbits: 1977. National Academies Press; 1977 Feb 1.

16. Garcia MR, Amstalden M, Morrison CD, Keisler, DH, and Williams GL. Age at puberty, total fat and conjugated linoleic acid content of carcass, and circulating metabolic hormones in beef heifers fed a diet high in linoleic acid beginning at four months of age. J. Anim. Sci. 2003; 81(1), 261–268. doi: 10.2527/2003.811261x 12597397

17. AOAC. Official methods of analysis. 17th ed., Vols. 1 and 2. Gaithersburg, MD: Association of the Official Analytical Chemists, 2000.

18. Macari M, and Machado CR. Sexual maturity in rabbits defined by the physical and chemical characteristics of the semen. Laboratory Animals. 1978; 12: 37–39. doi: 10.1258/002367778780953305 625098

19. Boulbina I, Ain-Baziz H, Ilès I, Zenia S, Belabbas R, Temim S. Effect of birth season on onset of puberty and semen characteristics in male rabbit of Algerian population (Oryctolagus cuniculus). World Rabbit Science Association Proceedings 10 th World Rabbit Congress. 2012; 335–339.

20. Viudes-de-Castro MP, Lavara R, Safaa HM, Marco-Jiménez F, Mehaisen GMK, and Vicente JS. Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits. Animal. 2014; 8(5), pp. 765–770. doi: 10.1017/S1751731114000135 24534625

21. Perumal P. Effect of superoxide dismutase on semen parameters and antioxidant enzyme activities of liquid stored (5 C) Mithun (Bos frontalis) semen. Journal of Animals. 2014; 1–9. doi: 10.3390/ani5010001

22. Simón L, Funes AK, Yapur MA, Cabrillana ME, Monclus MA, Boarelli PV, et al. Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model. PloS one. 2017; 12(2):e0172994. doi: 10.1371/journal.pone.0172994 28241054

23. Ijaz A, Javed I, Aslam B, Khan JA, Khaliq T, Khan MZ, et al. Nephroprotective and antioxidant effects of Moringa Oleifera (Sohanjna) in paracetamol induced nephrotoxic albino rabbits. Pak. Vet. J. 2016; 36(3): 292–296.

24. Ahmed-Farid OA, Nasr M, Ahmed RF, and Bakeer RM. Beneficial effects of curcumin nano-emulsion on spermatogenesis and reproductive performance in male rats under protein deficient diet model: enhancement of sperm motility, conservancy of testicular tissue integrity, cell energy and seminal plasma amino acids content. Journal of Biomedical Science. 2017; 24(1), p. 66. doi: 10.1186/s12929-017-0373-5 28865467

25. Ling B, Aziz C, and Alcorn J. Systematic evaluation of key L-Carnitine homeostasis mechanisms during postnatal development in rat. Nutrition & Metabolism. 2012; 1:66.

26. McMurray CH, Blanchflower WJ, and Rice DA. Influence of extraction techniques on determination of alpha tocopherol in animal feedstuffs. Journal-Association of Official Analytical Chemists. 1980; 63(6), pp. 1258–1261. 7451387

27. Abdelatty AM, Mohamed SA, Moustafa MMA, Al-Mokaddem AK, Baker MR, Elolimy AA, et al. Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit. PLoS ONE. 2019; 14(10): e0222404. https://doi.org/10.1371/journal.pone.0222404 31600212

28. O'fallon JV, Busboom JR, Nelson ML, and Gaskins CT. A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J. Anim. Sci. 2007; 85(6), pp. 1511–1521. doi: 10.2527/jas.2006-491 17296772

29. Sirri F, Castellini C, Roncarati A, Franchini A, and Meluzzi A. Effect of feeding and genotype on the lipid profile of organic chicken meat. European Journal of Lipid Science and Technology. 2010; 112(9), pp. 994–1002.

30. Aslan Koşar P, Tuncer H, Cihangir Uğuz A, Espino Palma J, Darıcı H, Onaran İ, Çiğ B, et al. The efficiency of Poly (ADP‐Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion. International Journal of Experimental Pathology. 2015. 96(5), pp. 294–300. doi: 10.1111/iep.12137 26303136

31. Sun Y, Wolcott RD, and Dowd SE. Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment. In High-Throughput Next Generation Sequencing. 2011. pp. 129–141. Humana Press, Totowa, NJ.

32. Norollahi SA, Kokhaee P, Rashidy-Pour A, Hojati V, Norollahi SE, Larijani LV, and Samadan AA. Comparison of Methods of RNA Extraction From Breast and Gastric Cancer Tissues. Crescent J. Med. Biol. Sci. 2018; 5(1), pp. 25–28.

33. Bionaz M and Loor JJ. Identification of reference genes for quantitative real-time PCR in the bovine mammary gland during the lactation cycle. Physiological Genomics. 2007; 29(3), 312–319. doi: 10.1152/physiolgenomics.00223.2006 17284669

34. Andersen CL, Jensen JL, and Ørntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research. 2004; 64(15), pp. 5245–5250. doi: 10.1158/0008-5472.CAN-04-0496 15289330

35. Wiegand BR, Sparks JC, Parrish FC, and Zimmerman, DR. Duration of feeding conjugated linoleic acid influences growth performance, carcass traits, and meat quality of finishing barrows. Journal of Animal Science. 2002; 80 (3), pp. 637–643. doi: 10.2527/2002.803637x

36. Moraes ML, Ribeiro AML, Santin E, and Klasing KC. Effects of conjugated linoleic acid and lutein on the growth performance and immune response of broiler chickens. Poultry Science. 2015; 95(2), pp. 237–246. doi: 10.3382/ps/pev325 26527712

37. Koronowicz AA, and Banks P. Antitumor properties of CLA-enriched food products. Nutrition and Cancer. 2018; 70(4), pp. 529–545. doi: 10.1080/01635581.2018.1460684 29697270

38. Onankpoya I, Posadzki P, Watson L, Davies L, and Ernst E. Efficacy of long-term conjugated linoleic acid (CLA) supplementation in the management of overweight and obesity: a systematic review and meta-analysis of randomized clinical trials. European Journal of Nutrition. 2011; 51(2), 127–134. doi: 10.1007/s00394-011-0253-9

39. Lauridsen C, Mu H, and Henckel P. Influence of dietary conjugated linoleic acid (CLA) and age at slaughtering on performance, slaughter-and meat quality, lipoproteins, and tissue deposition of CLA in barrows. Meat Science. 2005; 69(3), pp. 393–399. doi: 10.1016/j.meatsci.2004.08.009 22062976

40. Szymczyk B, Pisulewski P, Szczurek W, and Hanczakowski P, The effects of feeding conjugated linoleic acid (CLA) on rat growth performance, serum lipoproteins and subsequent lipid composition of selected rat tissues. Journal of the Science of Food and Agriculture. 2000; 80(10), pp. 1553–1558.

41. Lindsey CE. Influence of conjugated linoleic acid supplementation on body composition of weaned pigs. Ph.D. thesis, Sam Houston State University. 2017. Available from: https://shsu-ir.tdl.org/handle/20.500.11875/2188

42. Liu H, Wu F, Bai LL, Chen YF, Lai CH, Ren LQ et al. Effect of dietary conjugated linoleic acid supplementation during late gestation on colostrum yield, fatty acid composition, and IgG concentrations in primiparous sows. Canadian Journal of Animal Science. 2018; 98(4), pp. 732–740.

43. Larsen TM, Toubro S, Gudmundsen O, and Astrup A. Conjugated linoleic acid supplementation for 1 y does not prevent weight or body fat regain. The American Journal of Clinical Nutrition. 2006; 83(3), pp. 606–612. doi: 10.1093/ajcn.83.3.606 16522907

44. Racine NM, Watras AC, Carrel AL, Allen DB, McVean JJ, Clark RR. Effect of conjugated linoleic acid on body fat accretion in overweight or obese children. The American Journal of Clinical Nutrition. 2010; 91(5), pp. 1157–1164. doi: 10.3945/ajcn.2009.28404 20200257

45. Gaullier JM, Halse J, Høye K, Kristiansen K, Fagertun H, Vik H, et al. Conjugated linoleic acid supplementation for 1 y reduces body fat mass in healthy overweight humans. The American Journal of Clinical Nutrition. 2004; 79(6), pp. 1118–1125. doi: 10.1093/ajcn/79.6.1118 15159244

46. Gaullier JM, Halse J, Høivik HO, Høye K, Syvertsen C, Nurminiemi Met al. Six months supplementation with conjugated linoleic acid induces regional-specific fat mass decreases in overweight and obese. British Journal of Nutrition. 2007; 97(3), pp. 550–560. doi: 10.1017/S0007114507381324

47. Corino C, Mourot J, Magni S, Pastorelli G, and Rosi F. Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits. Journal of Animal Science. 2002; 80(4), pp. 1020–1028. doi: 10.2527/2002.8041020x 12002308

48. Vernet P, Aitken RJ, and Drevet JR. Antioxidant strategies in the epididymis. Molecular and Cellular Endocrinology. 2004; 216(1–2), pp. 31–39. doi: 10.1016/j.mce.2003.10.069 15109742

49. Marco-Jiménez F, Vicente JS. Overweight in young males reduce fertility in rabbit model. PLoS ONE. 2017; 12(7): e0180679 doi: 10.1371/journal.pone.0180679 28700645

50. Abd-Allah AR, Helal GK, Al-Yahya AA, Aleisa AM, Al-Rejaie SS, et al. Pro-inflammatory and oxidative stress pathways which compromise sperm motility and survival may be altered by L-carnitine. Oxidative Medicine and Cellular Longevity. 2009; 2(2), pp. 73–81. doi: 10.4161/oxim.2.2.8177 20357928

51. Castellini C, Lattaioli P, Dal Bosco A, Minelli A, and Mugnai C. Oxidative status and semen characteristics of rabbit buck as affected by dietary. Reproduction Nutrition Development. 2003; 43(1), pp. 91–103.

52. Chen WH, Li YJ, Wang MS, Kang ZC, Huang HL, and Shaw HM. Elevation of tissue α-tocopherol levels by conjugated linoleic acid in C57BL/6J mice is not associated with changes in vitamin E absorption or α-carboxyethyl hydroxychroman production. Nutrition. 2012; 28(1), pp. 59–66. doi: 10.1016/j.nut.2011.04.006 21872434

53. Zeitz JO, Most E, and Eder K. Conjugated linoleic acid influences the metabolism of tocopherol in lactating rats but has little effect on tissue tocopherol concentrations in pups. Lipids in Health and Disease. 2016; 15(1), p. 102.

54. Sessler AM, Kaur N, Palta JP, and Ntambi JM. Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes. Journal of Biological Chemistry. 1996; 271(47), pp. 29854–29858. doi: 10.1074/jbc.271.47.29854 8939925

55. Ntambi JM. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lipid Research. 1999; 40(9), pp. 1549–1558. 10484602

56. Brown JM, Boysen MS, Jensen SS, Morrison RF, Storkson J, Lea Currie R, et al. Isomer-specific regulation of metabolism and PPARγ signaling by CLA in human preadipocytes. Journal of Lipid Research. 2003; 44(7), pp. 1287–1300. doi: 10.1194/jlr.M300001-JLR200 12730300

57. Ramiah SK, Meng GY, Sheau Wei T, Swee Keong Y, and Ebrahimi M. Dietary conjugated linoleic acid supplementation leads to downregulation of PPAR transcription in broiler chickens and reduction of adipocyte cellularity. PPAR Research, 2014; 1–10. doi: 10.1155/2014/137652 25309587

58. Hue JJ, Lee KN, Jeong JH, Lee SH, Lee YH, Jeong SW, Nam SY, Yun YW and Lee BJ. Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice. Journal of Veterinary Science. 2009; 10(3), pp. 189–195. doi: 10.4142/jvs.2009.10.3.189 19687618

59. Liu KL and Belury MA. Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Letters.1998; 127(1–2), pp. 15–22. doi: 10.1016/s0304-3835(97)00479-5 9619853

60. Creasy D, Bube A, Rijk ED, Kandori H, Kuwahara M, Masson R. Proliferative and non-proliferative lesions of the rat and mouse male reproductive system. Toxicol. Pathol. 2012; 40:40S–121S; doi: 10.1177/0192623312454337 22949412

61. Mahdavi R, Faramarzi E, Mohammad-Zadeh M, Nasirimotlagh B, and Ghaemmaghami SJ. Effects of conjugated linoleic acid supplementation on nutritional status, symptoms of eating problems and dietary intake in rectal cancer patients undergoing chemoradiotherapy. Current Topics in Nutraceutical Research. 2013; 11(1–2), pp. 15–21.

62. Dym M and Madhwa Raj HG. Response of adult rat sertoli cells and leydig cells to depletion of luteinizing hormone and testosterone. Biology of Reproduction. 1977; 17(5), pp. 676–696. doi: 10.1095/biolreprod17.5.676 597535

63. Li J and Yuan J. Caspases in apoptosis and beyond. Oncogene. 2008; 27(48), p. 6194. doi: 10.1038/onc.2008.297 18931687

64. Mutomba MC, Yuan H, Konyavko M, Adachi S, Yokoyama CB, Esser V, et al. Regulation of the activity of caspases by L‐carnitine and palmitoyl carnitine. FEBS Letters. 2000; 478(1–2), pp. 19–25. doi: 10.1016/s0014-5793(00)01817-2 10922462

65. Miner JL, Cederberg CA, Nielsen MK, Chen X, and Baile CA. Conjugated linoleic acid (CLA), body fat, and apoptosis. Obesity Research. 2001; 9(2), pp. 129–134. doi: 10.1038/oby.2001.16 11316347

66. Nazari M, Karandish M, Jalali MT, and Saberi AH. Weight management effects of a mixture of conjugated linoleic acid and L-carnitine in diet induced obese rats. British Journal of Medicine and Medical Research. 2016; 12(1): 1–8. doi: 10.9734/BJMMR/2016/21609

67. Bezan P, Holland H, de Castro G, Cardoso J, Ovidio P, Calder P, et al. High dose of a conjugated linoleic acid mixture increases insulin resistance in rats fed either a low fat or a high fat diet. Exp. Clin. Endocrinol. Diabetes. 2018; 126, 379–386. doi: 10.1055/s-0043-118348 29388176

68. Parra P, Palou A, and Serra F. Moderate doses of conjugated linoleic acid reduce fat gain, maintain insulin sensitivity without impairing inflammatory adipose tissue status in mice fed a high-fat diet. Nutrition & Metabolism. 2010; 7(1), 5. doi: 10.1186/1743-7075-7-5 20180981

69. Srinivasan V, Thombre DP, Lakshmanan S, and Chakrabarty AS. Effect of removal of epididymal fat on spermatogenesis in albino rats. Indian Journal of Experimental Biology.1986; 24(8), pp. 487–488. 3817886

70. Modaresi M, Mansouri A, and Khodaei HR. The effect of conjugated linoleic acid on male reproductive hormones in mice. Advance Journal of Food Science & Technology. 2013; 5(12), pp. 1618–1620.

71. Baartscheer A, Schumacher CA, Wüst RCI, Fiolet JWT, Stienen GJM, Coronel R, et al. Empagliflozin decreases myocardial cytoplasmic Na+ through inhibition of the cardiac Na+/H+ exchanger in rats and rabbits. Diabetologia. 2016; 60(3), 568–573. doi: 10.1007/s00125-016-4134-x 27752710

72. Blay RM, Adjenti SK, Adutwum-Ofosu KK, Hottor BA, Ahenkorah J, Arko-Boham B, et al. Natural cocoa inhibits maternal hypercholesterolaemia-induced atherogenesis in rabbit pups. Cardiovascular Journal of Africa. 2019; 30(4): 208–215. doi: 10.5830/CVJA-2019-019 31140539

73. Sun X, Shen J, Liu C, Li S, Peng Y, Chen C et al. L-arginine and N-carbamoyl glutamic acid supplementation enhance young rabbit growth and immunity by regulating intestinal microbial community. Asian-Australas J. Anim. Sci. 2019. Available from: https://www.ajas.info/journal/view.php? doi: 10.5713/ajas.18.0984

74. Werner RA, Kobayashi R, Javadi MS, Köck Z, Wakabayashi H, Unterecker S. et al. Impact of novel antidepressants on cardiac 123i-metaiodobenzylguanidine uptake: experimental studies on SK-N-SH cells and healthy rabbits. Journal of Nuclear Medicine, 59(7), 1099–1103. doi: 10.2967/jnumed.117.206045 29496989

75. Levingstone TJ, Thompson E, Matsiko A, Schepens A, Gleeson JP, O’Brien FJ. Multi-layered collagen-based scaffolds for osteochondral defect repair in rabbits. Acta Biomaterialia. 2016; 32, 149–160. doi: 10.1016/j.actbio.2015.12.034 26724503

76. Torrado J, Cain C, Mauro AG, Romeo F, Ockaili R, Chau VQ, et al. Sacubitril/valsartan averts adverse post-infarction ventricular remodeling and preserves systolic function in rabbits. Journal of the American College of Cardiology. 2018; 72(19), 2342–2356. doi: 10.1016/j.jacc.2018.07.102 30384891

77. Ma H, Zheng L, Liu Y, Zhao C, Harrison TJ, Ma Y, et al. Experimental infection of rabbits with rabbit and genotypes 1 and 4 hepatitis E viruses. PLoS ONE.2010; 5(2): e9160. https://doi.org/10.1371/journal.pone.0009160 20161794

Článek Disparate effects of antibiotic-induced microbiome change and enhanced fitness in Daphnia magna
Článek Deliver on Your Own: Disrespectful Maternity Care in rural Kenya
Článek Risk of complications among diabetics self-reporting oral health status in Canada: A population-based cohort study
Článek Practical considerations in the use of a porcine model (Sus scrofa domesticus) to assess prevention of postoperative peritubal adhesions
Článek Transcriptional Differences in Peanut (Arachis hypogaea L.) Seeds at the Freshly Harvested, After-ripening and Newly Germinated Seed Stages: Insights into the Regulatory Networks of Seed Dormancy Release and Germination
Článek Identifying maintenance hosts for infection with Dichelobacter nodosus in free-ranging wild ruminants in Switzerland: A prevalence study
Článek Model order reduction for left ventricular mechanics via congruency training
Článek Production, purification and evaluation of biodegradation potential of PHB depolymerase of Stenotrophomonas sp. RZS7
Článek Seroprevalence of viral and vector-borne bacterial pathogens in domestic dogs (Canis familiaris) in northern Botswana
Článek Musical expertise generalizes to superior temporal scaling in a Morse code tapping task
Článek Cross-cultural adaptation and psychometric evaluation of the Yoruba version of Oswestry disability index
Článek Post-transcriptional regulation of Rad51c by miR-222 contributes cellular transformation
Článek Can scientists fill the science journalism void? Online public engagement with science stories authored by scientists
Článek Retention and predictors of attrition among patients who started antiretroviral therapy in Zimbabwe’s national antiretroviral therapy programme between 2012 and 2015
Článek Prognostics for pain in osteoarthritis: Do clinical measures predict pain after total joint replacement?
Článek Effects of Transcranial Direct Current Stimulation on GABA and Glx in Children: A pilot study
Článek Evaluation of rice wild relatives as a source of traits for adaptation to iron toxicity and enhanced grain quality
Článek Brief communication: Long-term absence of Langerhans cells alters the gene expression profile of keratinocytes and dendritic epidermal T cells
Článek APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression
Článek Morphological diversity within a core collection of subterranean clover (Trifolium subterraneum L.): Lessons in pasture adaptation from the wild
Článek Feasibility of real-time in vivo 89Zr-DFO-labeled CAR T-cell trafficking using PET imaging
Článek Repository-based plasmid design
Článek A new method of recording from the giant fiber of Drosophila melanogaster shows that the strength of its auditory inputs remains constant with age
Článek Aberrant cervical innate immunity predicts onset of dysbiosis and sexually transmitted infections in women of reproductive age
Článek Safe mobility, socioeconomic inequalities, and aging: A 12-year multilevel interrupted time-series analysis of road traffic death rates in a Latin American country
Článek THAP11F80L cobalamin disorder-associated mutation reveals normal and pathogenic THAP11 functions in gene expression and cell proliferation
Článek Lesion of striatal patches disrupts habitual behaviors and increases behavioral variability
Článek A clinical method for estimating the modulus of elasticity of the human cornea in vivo
Článek Patient perceived value of teleophthalmology in an urban, low income US population with diabetes
Článek Evidence in support of chromosomal sex influencing plasma based metabolome vs APOE genotype influencing brain metabolome profile in humanized APOE male and female mice
Článek Accelerated sparsity based reconstruction of compressively sensed multichannel EEG signals
Článek Microvesicles from Lactobacillus reuteri (DSM-17938) completely reproduce modulation of gut motility by bacteria in mice
Článek Gamma Knife radiosurgery for vestibular schwannomas: Evaluation of planning using the sphericity degree of the target volume
Článek Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)
Článek Why are animal source foods rarely consumed by 6-23 months old children in rural communities of Northern Ethiopia? A qualitative study
Článek A study to better understand under-utilization of laboratory tests for antenatal care in Senegal
Článek Effectiveness of information technology–enabled ‘SMART Eating’ health promotion intervention: A cluster randomized controlled trial
Článek Cauda Equina Syndrome Core Outcome Set (CESCOS): An international patient and healthcare professional consensus for research studies
Článek A new species of Macrocypraea (Gastropoda, Cypraeidae) from Trindade Island, Brazil, including phenotypic differentiation from remaining congeneric species
Článek A new approach to the temporal significance of house orientations in European Early Neolithic settlements
Článek Persistence of chikungunya ECSA genotype and local outbreak in an upper medium class neighborhood in Northeast Brazil
Článek In vivo elongation of thin filaments results in heart failure
Článek Disparity in depressive symptoms between heterosexual and sexual minority men in China: The role of social support
Článek Effect of classroom intervention on student food selection and plate waste: Evidence from a randomized control trial
Článek Mating strategy is determinant of adenovirus prevalence in European bats
Článek Preventing HIV and HSV-2 through knowledge and attitudes: A replication study of a multi-component community-based intervention in Zimbabwe
Článek Randomized clinical trial analyzing maintenance of peripheral venous catheters in an internal medicine unit: Heparin vs. saline
Článek Patient-related factors may influence nursing perception of sleep in the Intensive Care Unit
Článek A randomized trial of a behavioral intervention to decrease hospital length of stay by decreasing bedrest
Článek Color image segmentation using adaptive hierarchical-histogram thresholding
Článek The role of demographic history and selection in shaping genetic diversity of the Galápagos penguin (Spheniscus mendiculus)
Článek Attitudes towards animal study registries and their characteristics: An online survey of three cohorts of animal researchers
Článek Risk perception and behavioral change during epidemics: Comparing models of individual and collective learning
Článek Risk factors for third-generation cephalosporin resistant Enterobacteriaceae in gestational urine cultures: A retrospective cohort study based on centralized electronic health records
Článek Residential neighbourhood greenspace is associated with reduced risk of cardiovascular disease: A prospective cohort study
Článek Prevention and control of cholera with household and community water, sanitation and hygiene (WASH) interventions: A scoping review of current international guidelines
Článek Female finches prefer courtship signals indicating male vigor and neuromuscular ability
Článek The effect of spatial position and age within an egg-clutch on embryonic development and key metabolic enzymes in two clownfish species, Amphiprion ocellaris and Amphiprion frenatus
Článek The impact of translated reminder letters and phone calls on mammography screening booking rates: Two randomised controlled trials
Článek Application of a genetic algorithm to the keyboard layout problem
Článek Relationship between diabetic macular edema and choroidal layer thickness
Článek Evaluation of the predictive ability of ultrasound-based assessment of breast cancer using BI-RADS natural language reporting against commercial transcriptome-based tests
Článek A Comprehensive Data Gathering Network Architecture in Large-Scale Visual Sensor Networks
Článek Recovery of health-related quality of life after burn injuries: An individual participant data meta-analysis
Článek Role of ecology in shaping external nasal morphology in bats and implications for olfactory tracking
Článek High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer
Článek Development and validation of a prognostic model predicting symptomatic hemorrhagic transformation in acute ischemic stroke at scale in the OHDSI network
Článek Complex patterns of cell growth in the placenta in normal pregnancy and as adaptations to maternal diet restriction
Článek Tofu intake is inversely associated with risk of breast cancer: A meta-analysis of observational studies
Článek Influence of light on the infection of Aureococcus anophagefferens CCMP 1984 by a “giant virus”
Článek Temporal ordering of input modulates connectivity formation in a developmental neuronal network model of the cortex
Článek From organ to cell: Multi-level telomere length assessment in patients with idiopathic pulmonary fibrosis
Článek How do critical care staff respond to organisational challenge? A qualitative exploration into personality types and cognitive processing in critical care
Článek Effects of supplemental creatine and guanidinoacetic acid on spatial memory and the brain of weaned Yucatan miniature pigs
Článek Community-Based Health Planning and Services Plus programme in Ghana: A qualitative study with stakeholders in two Systems Learning Districts on improving the implementation of primary health care
Článek An investigation of transportation practices in an Ontario swine system using descriptive network analysis
Článek Comparison of gridded precipitation datasets for rainfall-runoff and inundation modeling in the Mekong River Basin
Článek Functional interactions in patients with hemianopia: A graph theory-based connectivity study of resting fMRI signal
Článek The effects of dual-task cognitive interference on gait and turning in Huntington’s disease
Článek Novel imaging biomarkers for mapping the impact of mild mitochondrial uncoupling in the outer retina in vivo
Článek Hyperkalemia treatment modalities: A descriptive observational study focused on medication and healthcare resource utilization
Článek Long term impact of PositiveLinks: Clinic-deployed mobile technology to improve engagement with HIV care
Článek Comparison of post-transplantation diabetes mellitus incidence and risk factors between kidney and liver transplantation patients
Článek A definition-by-example approach and visual language for activity patterns in engineering disciplines
Článek A network analysis revealed the essential and common downstream proteins related to inguinal hernia
Článek Use of conventional cardiac troponin assay for diagnosis of non-ST-elevation myocardial infarction: ‘The Ottawa Troponin Pathway’
Článek Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells
Článek Research on motion planning for an indoor spray arm based on an improved potential field method
Článek Detailed analysis of the transverse arch of hallux valgus feet with and without pain using weightbearing ultrasound imaging and precise force sensors
Článek Surrogate R-spondins for tissue-specific potentiation of Wnt Signaling
Článek Apolipoprotein-AI mimetic peptides D-4F and L-5F decrease hepatic inflammation and increase insulin sensitivity in C57BL/6 mice
Článek Treating patients with driving phobia by virtual reality exposure therapy – a pilot study
Článek Efficient processing of raster and vector data
Článek Therapeutic hypothermia after out of hospital cardiac arrest improve 1-year survival rate for selective patients
Článek Carotid plaques and neurological impairment in patients with acute cerebral infarction
Článek Deep learning based image reconstruction algorithm for limited-angle translational computed tomography
Článek Association between coffee drinking and telomere length in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
Článek Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats
Článek Rad51 paralogs and the risk of unselected breast cancer: A case-control study
Článek Diagnostic differences in respiratory breathing patterns and work of breathing indices in children with Duchenne muscular dystrophy
Článek The role of narrative in collaborative reasoning and intelligence analysis: A case study
Článek Proportions of CD4 test results indicating advanced HIV disease remain consistently high at primary health care facilities across four high HIV burden countries
Článek Modelling of amino acid turnover in the horse during training and racing: A basis for developing a novel supplementation strategy
Článek Single-modal and multi-modal false arrhythmia alarm reduction using attention-based convolutional and recurrent neural networks
Článek Eye-gaze information input based on pupillary response to visual stimulus with luminance modulation
Článek Trends of litter decomposition and soil organic matter stocks across forested swamp environments of the southeastern US
Článek Post mortem evaluation of inflammation, oxidative stress, and PPARγ activation in a nonhuman primate model of cardiac sympathetic neurodegeneration
Článek Disruption in daily eating-fasting and activity-rest cycles in Indian adolescents attending school
Článek Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss)
Článek Percent amplitude of fluctuation: A simple measure for resting-state fMRI signal at single voxel level
Článek Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women
Článek Are changes in depressive symptoms, general health and residential area socio-economic status associated with trajectories of waist circumference and body mass index?
Článek Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
Článek Link-centric analysis of variation by demographics in mobile phone communication patterns
Článek Tobacco smoking and health-related quality of life among university students: Mediating effect of depression
Článek Incidence of hospital-acquired pressure ulcers in patients with "minimal risk" according to the "Norton-MI" scale
Článek Lipoprotein(a) plasma levels are not associated with survival after acute coronary syndromes: An observational cohort study
Článek Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine
Článek Biochemical characterization of Ty1 retrotransposon protease
Článek Lateral pressure equalisation as a principle for designing support surfaces to prevent deep tissue pressure ulcers
Článek The validation of the Beijing version of the Montreal Cognitive Assessment in Chinese patients undergoing hemodialysis
Článek Inflammasome expression is higher in ovarian tumors than in normal ovary
Článek HCV genotype profile in Brazil of mono-infected and HIV co-infected individuals: A survey representative of an entire country
Článek Engaging with change: Information and communication technology professionals’ perspectives on change at the mid-point in the UK/EU Brexit process
Článek Adherence to iron-folic acid supplement and associated factors among antenatal care attending pregnant mothers in governmental health institutions of Adwa town, Tigray, Ethiopia: Cross-sectional study
Článek Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae
Článek Development of a risk score for prediction of poor treatment outcomes among patients with multidrug-resistant tuberculosis
Článek Preclinical evaluation of AT-527, a novel guanosine nucleotide prodrug with potent, pan-genotypic activity against hepatitis C virus
Článek Aqueous extract from Mangifera indica Linn. (Anacardiaceae) leaves exerts long-term hypoglycemic effect, increases insulin sensitivity and plasma insulin levels on diabetic Wistar rats
Článek Discovery of Jogalong virus, a novel hepacivirus identified in a Culex annulirostris (Skuse) mosquito from the Kimberley region of Western Australia
Článek Detection of Torque Teno Virus (TTV) and TTV-Like Minivirus in patients with presumed infectious endophthalmitis in India
Článek CD4 rate of increase is preferred to CD4 threshold for predicting outcomes among virologically suppressed HIV-infected adults on antiretroviral therapy
Článek Estimating the basic reproduction number of a pathogen in a single host when only a single founder successfully infects
Článek What drugs modify the risk of iatrogenic impulse-control disorders in Parkinson’s disease? A preliminary pharmacoepidemiologic study
Článek Evaluating emotional distress and health-related quality of life in patients with heart failure and their family caregivers: Testing dyadic dynamics using the Actor-Partner Interdependence Model
Článek Catchment-scale export of antibiotic resistance genes and bacteria from an agricultural watershed in central Iowa
Článek Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay
Článek Proof-of-concept for a non-invasive, portable, and wireless device for cardiovascular monitoring in pediatric patients
Článek On PTV definition for glioblastoma based on fiber tracking of diffusion tensor imaging data
Článek Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers
Článek Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells
Článek Evaluation of a bioengineered ACL matrix’s osteointegration with BMP-2 supplementation
Článek Psychosocial profiles of physical activity fluctuation in office employees: A latent profile analysis
Článek Soluble AXL as a marker of disease progression and survival in melanoma
Článek Gender differences influence over insomnia in Korean population: A cross-sectional study
Článek Impact of scion/rootstock reciprocal effects on metabolomics of fruit juice and phloem sap in grafted Citrus reticulata
Článek Adapting cognitive diagnosis computerized adaptive testing item selection rules to traditional item response theory
Článek Autumn shifts in cold tolerance metabolites in overwintering adult mountain pine beetles
Článek Umbilical cord separation time, predictors and healing complications in newborns with dry care
Článek Analysis of attitudinal components towards statistics among students from different academic degrees
Článek Effects of fatigue induced by repeated-sprint on kicking accuracy and velocity in female soccer players
Článek A pre-clinical validation plan to evaluate analytical sensitivities of molecular diagnostics such as BD MAX MDR-TB, Xpert MTB/Rif Ultra and FluoroType MTB
Článek Leadership for success in transforming medical abortion policy in Canada
Článek Clinical correlates associated with the long-term response of bipolar disorder patients to lithium, valproate or lamotrigine: A retrospective study
Článek Forecasting stock prices with long-short term memory neural network based on attention mechanism
Článek On the genus Crossaster (Echinodermata: Asteroidea) and its distribution
Článek Intracellular and in vivo evaluation of imidazo[2,1-b]thiazole-5-carboxamide anti-tuberculosis compounds
Článek An integrated vitamin E-coated polymer hybrid nanoplatform: A lucrative option for an enhanced in vitro macrophage retention for an anti-hepatitis B therapeutic prospect
Článek The effect of strontium and silicon substituted hydroxyapatite electrochemical coatings on bone ingrowth and osseointegration of selective laser sintered porous metal implants
Článek Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma species in dogs with hemangiosarcoma from across the United States
Článek Color discrimination and gas chromatography-mass spectrometry fingerprint based on chemometrics analysis for the quality evaluation of Schizonepetae Spica
Článek Comparisons of recurrence-free survival and overall survival between microwave versus radiofrequency ablation treatment for hepatocellular carcinoma: A multiple centers retrospective cohort study with propensity score matching
Článek Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution
Článek Assertive, trainable and older dogs are perceived as more dominant in multi-dog households
Článek Prediction of Uropathogens by Flow Cytometry and Dip-stick Test Results of Urine Through Multivariable Logistic Regression Analysis
Článek Interleukin 6 is increased in preclinical HNSCC models of acquired cetuximab resistance, but is not required for maintenance of resistance
Článek Impact of viral disease hypophagia on pig jejunal function and integrity
Článek Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia
Článek Evaluation and validation of 2D biomechanical models of the knee for radiograph-based preoperative planning in total knee arthroplasty
Článek Soil-Transmitted Helminth infections reduction in Bhutan: A report of 29 years of deworming
Článek Fast quantitative time lapse displacement imaging of endothelial cell invasion
Článek Two novel mutations in MSX1 causing oligodontia
Článek Dome-shaped macula in children and adolescents
Článek Targeted transcriptomic study of the implication of central metabolic pathways in mannosylerythritol lipids biosynthesis in Pseudozyma antarctica T-34
Článek Preliminary evidences of the presence of extracellular DNA single stranded forms in soil
Článek A comparison of quality of life between patients treated with different dialysis modalities in Taiwan
Článek Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection
Článek Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum
Článek Real-world data about emotional stress, disability and need for social care in a German IBD patient cohort
Článek The regenerative compatibility: A synergy between healthy ecosystems, environmental attitudes, and restorative experiences
Článek Perceptions of risk and influences of choice in pregnant women with obesity. An evidence synthesis of qualitative research
Článek Sexual risk classes among youth experiencing homelessness: Relation to childhood adversities, current mental symptoms, substance use, and HIV testing
Článek Effects of CK2β subunit down-regulation on Akt signalling in HK-2 renal cells
Článek Novel broad-spectrum activity-based probes to profile malarial cysteine proteases
Článek Association between opioid analgesic therapy and initiation of buprenorphine management: An analysis of prescription drug monitoring program data
Článek Improvement project in higher education institutions: A BPEP-based model
Článek An updated evaluation of serum sHER2, CA15.3, and CEA levels as biomarkers for the response of patients with metastatic breast cancer to trastuzumab-based therapies
Článek Genome-wide association study of metabolic syndrome in Korean populations
Článek Drug therapy problems and treatment satisfaction among ambulatory patients with epilepsy in a specialized hospital in Ethiopia
Článek Occurrence and distribution of anthropogenic persistent organic pollutants in coastal sediments and mud shrimps from the wetland of central Taiwan
Článek Intensified visual clutter induces increased sympathetic signalling, poorer postural control, and faster torsional eye movements during visual rotation
Článek Gut microbiota composition alterations are associated with the onset of diabetes in kidney transplant recipients
Článek Shock index and TIMI risk index as valuable prognostic tools in patients with acute coronary syndrome complicated by cardiogenic shock
Článek Merit overrules theory of mind when young children share resources with others
Článek Metabolic analysis of amino acids and vitamin B6 pathways in lymphoma survivors with cancer related chronic fatigue
Článek Immunopathogenesis of canine chronic ulcerative stomatitis
Článek Generalizing findings from a randomized controlled trial to a real-world study of the iLookOut, an online education program to improve early childhood care and education providers’ knowledge and attitudes about reporting child maltreatment
Článek When and what to test for: A cost-effectiveness analysis of febrile illness test-and-treat strategies in the era of responsible antibiotic use
Článek Comparison of effects and safety in providing controlled hypotension during surgery between dexmedetomidine and magnesium sulphate: A meta-analysis of randomized controlled trials
Článek The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
Článek Efficacy of a mitochondrion-targeting agent for reducing the level of urinary protein in rats with puromycin aminonucleoside-induced minimal-change nephrotic syndrome
Článek Association of endothelial nitric oxide synthase (NOS3) gene polymorphisms with primary open-angle glaucoma in a Saudi cohort
Článek Antitrust analysis with upward pricing pressure and cost efficiencies
Článek Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding
Článek Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum
Článek Sofosbuvir-based regimen for genotype 2 HCV infected patients in Taiwan: A real world experience
Článek The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function
Článek Sequencing artifacts derived from a library preparation method using enzymatic fragmentation
Článek Quantitative analysis of adsorption and desorption of volatile organic compounds on reusable zeolite filters using gas chromatography
Článek Quo vadis Pantanal? Expected precipitation extremes and drought dynamics from changing sea surface temperature
Článek The Brief Measure of Emotional Preoperative Stress (B-MEPS) as a new predictive tool for postoperative pain: A prospective observational cohort study
Článek The impact of diabetes mellitus medication on the incidence of endogenous endophthalmitis
Článek Clinical and pathological features of thrombotic microangiopathy influencing long-term kidney transplant outcomes
Článek Morphological adjustment in free-living Steinernema feltiae infective juveniles to increasing concentration of Nemafric-BL phytonematicide
Článek Key necroptotic proteins are required for Smac mimetic-mediated sensitization of cholangiocarcinoma cells to TNF-α and chemotherapeutic gemcitabine-induced necroptosis
Článek Concurrent lipidomics and proteomics on malignant plasma cells from multiple myeloma patients: Probing the lipid metabolome
Článek Retraction: SDR9C7 Promotes Lymph Node Metastases in Patients with Esophageal Squamous Cell Carcinoma
Článek Association between tuberculosis and depression on negative outcomes of tuberculosis treatment: A systematic review and meta-analysis
Článek Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system
Článek Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand
Článek Sex-specific and opposite modulatory aspects revealed by PPI network and pathway analysis of ischemic stroke in humans
Článek Control of the microsporidian parasite Nosema ceranae in honey bees (Apis mellifera) using nutraceutical and immuno-stimulatory compounds
Článek Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates
Článek Oral magnesium supplementation for leg cramps in pregnancy—An observational controlled trial
Článek Health care professionals’ knowledge of commonly used sedative, analgesic and neuromuscular drugs: A single center (Rambam Health Care Campus), prospective, observational survey
Článek Campylobacter portucalensis sp. nov., a new species of Campylobacter isolated from the preputial mucosa of bulls
Článek Transgenic interleukin 11 expression causes cross-tissue fibro-inflammation and an inflammatory bowel phenotype in mice
Článek Sleep quality and sex modify the relationships between trait energy and fatigue on state energy and fatigue
Článek The role of peer, parental, and school norms in predicting adolescents’ attitudes and behaviours of majority and different minority ethnic groups in Croatia
Článek Availability, prices and affordability of selected antibiotics and medicines against non-communicable diseases in western Cameroon and northeast DR Congo
Článek The effect of mutations derived from mouse-adapted H3N2 seasonal influenza A virus to pathogenicity and host adaptation
Článek Detection of posttraumatic pneumothorax using electrical impedance tomography—An observer-blinded study in pigs with blunt chest trauma
Článek Educators’ perceptions of organisational readiness for implementation of a pre-adolescent transdisciplinary school health intervention for inter-generational outcomes
Článek The effects of sport expertise and shot results on basketball players’ action anticipation
Článek Framework and algorithms for identifying honest blocks in blockchain
Článek Exploring the impact of terminology differences in blood and organ donor decision making
Článek Platelet indices significantly correlate with liver fibrosis in HCV-infected patients
Článek The nitrate content of fresh and cooked vegetables and their health-related risks
Článek Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants
Článek The 24-hour urinary cortisol in post-traumatic stress disorder: A meta-analysis
Článek “I like the way I am, but I feel like I could get a little bit bigger”: Perceptions of body image among adolescents and youth living with HIV in Durban, South Africa
Článek Nanoparticle-based ‘turn-on’ scattering and post-sample fluorescence for ultrasensitive detection of water pollution in wider window
Článek Insights into the strategy of micro-environmental adaptation: Transcriptomic analysis of two alvinocaridid shrimps at a hydrothermal vent
Článek Hyper-spectral response and estimation model of soil degradation in Kenli County, the Yellow River Delta
Článek Construction of a high-density genetic map and fine mapping of a candidate gene locus for a novel branched-spike mutant in barley
Článek Natural hybridization between Phyllagathis and Sporoxeia species produces a hybrid without reproductive organs
Článek Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia
Článek Characterization of black patina from the Tiber River embankments using Next-Generation Sequencing
Článek Validity of cerebrovascular ICD-9-CM codes in healthcare administrative databases. The Umbria Data-Value Project
Článek Torque teno virus viral load is related to age, CMV infection and HLA type but not to Alzheimer's disease
Článek Associations of cigarette smoking and burden of thoracic aortic calcification in asymptomatic individuals: A dose-response relationship
Článek Transforming assessment of speech in children with cleft palate via online crowdsourcing
Článek Assessment of peritoneal microbial features and tumor marker levels as potential diagnostic tools for ovarian cancer
Článek Deficiency syndromes in top predators associated with large-scale changes in the Baltic Sea ecosystem
Článek Perceived relative social status and cognitive load influence acceptance of unfair offers in the Ultimatum Game
Článek Hepatitis B and C virus infection among HIV patients within the public and private healthcare systems in Chile: A cross-sectional serosurvey
Článek Retraction: Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis
Článek From seed to flour: Sowing sustainability in the use of cantaloupe melon residue (Cucumis melo L. var. reticulatus)
Článek Core Scientific Dataset Model: A lightweight and portable model and file format for multi-dimensional scientific data
Článek Accounting for measurement error to assess the effect of air pollution on omic signals
Článek Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1
Článek Barriers for tuberculosis case finding in Southwest Ethiopia: A qualitative study
Článek Genetic predisposition to celiac disease in Kazakhstan: Potential impact on the clinical practice in Central Asia
Článek A lower psoas muscle volume was associated with a higher rate of recurrence in male clear cell renal cell carcinoma
Článek Two angles of overqualification-the deviant behavior and creative performance: The role of career and survival job
Článek Cost-utility analysis of de-escalating biological disease-modifying anti-rheumatic drugs in patients with rheumatoid arthritis
Článek Efficient estimation of stereo thresholds: What slope should be assumed for the psychometric function?
Článek Learning efficient haptic shape exploration with a rigid tactile sensor array
Článek Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages
Článek Regional versus local wind speed and direction at a narrow beach with a high and steep foredune
Článek Fragmented QRS complex in patients with systemic lupus erythematosus at the time of diagnosis and its relationship with disease activity
Článek Severe thiamine deficiency in eastern Baltic cod (Gadus morhua)
Článek Transfer entropy as a variable selection methodology of cryptocurrencies in the framework of a high dimensional predictive model
Článek Psychometric validation of Czech version of the Sport Motivation Scale
Článek Correction: Multiple innate antibacterial immune defense elements are correlated in diverse ungulate species
Článek Recognition of personality disorder and anxiety disorder comorbidity in patients treated for depression in secondary psychiatric care
Článek Correction: Strategies for achieving high sequencing accuracy for low diversity samples and avoiding sample bleeding using illumina platform
Článek ETAPOD: A forecast model for prediction of black pod disease outbreak in Nigeria
Článek Number of days required to estimate physical activity constructs objectively measured in different age groups: Findings from three Brazilian (Pelotas) population-based birth cohorts
Článek Exploring the mechanism of olfactory recognition in the initial stage by modeling the emission spectrum of electron transfer
Článek The impact of a wireless audio system on communication in robotic-assisted laparoscopic surgery: A prospective controlled trial
Článek Dense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cells
Článek Physicians’ perspectives regarding non-medical switching of prescription medications: Results of an internet e-survey
Článek Potential socioeconomic impacts from ocean acidification and climate change effects on Atlantic Canadian fisheries
Článek Design and evaluation of a laboratory-based wheelchair castor testing protocol using community data
Článek Modeling aggressive market order placements with Hawkes factor models
Článek Healthy lifestyle index and its association with hypertension among community adults in Sri Lanka: A cross-sectional study
Článek Effects of Allium hookeri on gut microbiome related to growth performance in young broiler chickens
Článek Were ancient foxes far more carnivorous than recent ones?—Carnassial morphological evidence
Článek The Shapley value for a fair division of group discounts for coordinating cooling loads
Článek Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice
Článek Clinical, cytogenetic and molecular genetic characterization of a tandem fusion translocation in a male Holstein cattle with congenital hypospadias and a ventricular septal defect
Článek Community- and trophic-level responses of soil nematodes to removal of a non-native tree at different stages of invasion
Článek Association of ECG parameters with late gadolinium enhancement and outcome in patients with clinical suspicion of acute or subacute myocarditis referred for CMR imaging
Článek Impact of multi-drug resistant bacteria on economic and clinical outcomes of healthcare-associated infections in adults: Systematic review and meta-analysis
Článek Intraoperative computed tomography imaging for dose calculation in intraoperative electron radiation therapy: Initial clinical observations
Článek Simple non-mydriatic retinal photography is feasible and demonstrates retinal microvascular dilation in Chronic Obstructive Pulmonary Disease (COPD)
Článek Maternal depressive symptoms and children’s cognitive development: Does early childcare and child’s sex matter?
Článek Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada
Článek Using machine learning methods to determine a typology of patients with HIV-HCV infection to be treated with antivirals
Článek Oral misoprostol, low dose vaginal misoprostol, and vaginal dinoprostone for labor induction: Randomized controlled trial
Článek The association between dietary patterns before and in early pregnancy and the risk of gestational diabetes mellitus (GDM): Data from the Malaysian SECOST cohort
Článek cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection
Článek Spectral characteristics of urine from patients with end-stage kidney disease analyzed using Raman Chemometric Urinalysis (Rametrix)
Článek Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis
Článek Morphological association between the muscles and bones in the craniofacial region
Článek Antenatal depression and its association with adverse birth outcomes in low and middle-income countries: A systematic review and meta-analysis
Článek The role of refugee and migrant migration status on medication adherence: Mediation through illness perceptions
Článek Effect of a community-based approach of iron and folic acid supplementation on compliance by pregnant women in Kiambu County, Kenya: A quasi-experimental study
Článek Plasma kynurenines and prognosis in patients with heart failure
Článek Natural selection contributes to food web stability
Článek Cloud-computing and machine learning in support of country-level land cover and ecosystem extent mapping in Liberia and Gabon
Článek Correction: Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition
Článek Occupational exposure to particulate matter from air pollution in the outdoor workplaces in Almaty during the cold season
Článek Beyond the heterodimer model for mineralocorticoid and glucocorticoid receptor interactions in nuclei and at DNA
Článek Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results
Článek Non-gradient and genotype-dependent patterns of RSV gene expression
Článek Drug-eluting versus bare-metal stents for first myocardial infarction in patients with atrial fibrillation: A nationwide population-based cohort study
Článek Health-related quality of life among patients with type 2 diabetes mellitus in Eastern Province, Saudi Arabia: A cross-sectional study
Článek Thirty-day readmission after medical-surgical hospitalization for people who experience imprisonment in Ontario, Canada: A retrospective cohort study
Článek The association of telomere length and telomerase activity with adverse outcomes in older patients with non-ST-elevation acute coronary syndrome
Článek Alterations of aqueous humor Aβ levels in Aβ-infused and transgenic mouse models of Alzheimer disease
Článek The impact of peer attachment on prosocial behavior, emotional difficulties and conduct problems in adolescence: The mediating role of empathy
Článek Problem gambling, associations with comorbid health conditions, substance use, and behavioural addictions: Opportunities for pathways to treatment
Článek Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution
Článek Human-raptor conflict in rural settlements of Colombia
Článok vyšiel v časopise

PLOS One

2020 Číslo 1
Najčítanejšie tento týždeň
Najčítanejšie v tomto čísle
Kurzy

Zvýšte si kvalifikáciu online z pohodlia domova

Aktuální možnosti diagnostiky a léčby litiáz
nový kurz
Autori: MUDr. Tomáš Ürge, PhD.
Všetky kurzy
Prihlásenie
Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.

Prihlásenie

Nemáte účet?  Registrujte sa