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preLekára.sk / Odborné časopisy / PLOS Genetics / 2014 - 10

The Not5 Subunit of the Ccr4-Not Complex Connects Transcription and Translation

English version České info

In this work we show that, both in the nucleus and in the cytoplasm, Not5 plays a “bridging” role for RNA Polymerase II. In the cytoplasm, Not5 interacts with the mRNA encoding the largest subunit of RNA polymerase II Rpb1 and supports the association of a co-chaperone to newly produced protein, to keep it soluble and assembly competent. In the nucleus, Not5 interacts with the Rpb4 subunit of polymerase that is known to readily dissociate from the rest of the polymerase, and it is essential for Rpb4 to associate with mRNAs at the completion of transcription to contribute to translation and mRNA degradation in the cytoplasm. Hence our data define Not5 as a key player in the cross-talk between different stages of eukaryotic gene expression: Not5 impacts on production of polymerase, hence transcription, during translation, and on Rpb4 mRNA association, hence translation and mRNA degradation during transcription.

Vyšlo v časopise: The Not5 Subunit of the Ccr4-Not Complex Connects Transcription and Translation. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004569
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004569

Souhrn

In this work we show that, both in the nucleus and in the cytoplasm, Not5 plays a “bridging” role for RNA Polymerase II. In the cytoplasm, Not5 interacts with the mRNA encoding the largest subunit of RNA polymerase II Rpb1 and supports the association of a co-chaperone to newly produced protein, to keep it soluble and assembly competent. In the nucleus, Not5 interacts with the Rpb4 subunit of polymerase that is known to readily dissociate from the rest of the polymerase, and it is essential for Rpb4 to associate with mRNAs at the completion of transcription to contribute to translation and mRNA degradation in the cytoplasm. Hence our data define Not5 as a key player in the cross-talk between different stages of eukaryotic gene expression: Not5 impacts on production of polymerase, hence transcription, during translation, and on Rpb4 mRNA association, hence translation and mRNA degradation during transcription.

Zdroje

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