POT1a and Components of CST Engage Telomerase and Regulate Its Activity in

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Telomeres are required to stabilize the ends of linear chromosomes, and thus ensure genome integrity. Telomeric DNA is maintained though the action of both conventional and non-conventional DNA replication mechanisms. To ensure that chromosome ends are fully protected and fully replicated, telomeres dynamically oscillate between a closed (non-extendable) and an open (extendable) conformation throughout the cell cycle. The telomerase reverse transcriptase engages telomeres when they are in an extendable conformation. How this conversion occurs, how telomerase is recruited to the chromosome terminus and how telomerase action is terminated are unanswered questions. Here we provide evidence that POT1a, a telomerase accessory protein from the flowering plant Arabidopsis, helps to convert the telomere into a telomerase-extendable state through dynamic interactions with a critical telomere binding protein complex, and through stimulation of telomerase enzyme activity. The results of this study provide new insight into the regulation of telomeric DNA replication.

Vyšlo v časopise: POT1a and Components of CST Engage Telomerase and Regulate Its Activity in. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004738
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004738

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