Protein Phosphatase 4 Promotes Chromosome Pairing and Synapsis, and Contributes to Maintaining Crossover Competence with Increasing Age

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Meiosis creates gametes by distributing diploid genomes containing homologous chromosome pairs into daughter cells that receive only one of each chromosome. To segregate correctly at the first meiotic division, chromosomes must pair and synapse with their homologous partners, and undergo crossover recombination, which requires breaking and repairing the DNA strands of all chromosomes. How chromosomes recognize their partners, and how a cell controls the amount of DNA breakage and recombination that occurs, are open questions. In this study, we observed meiosis in the nematode Caenorhabditis elegans to examine the role of Protein Phosphatase 4 (PP4). We found that in the absence of PP4, chromosomes often paired and synapsed with non-homologous chromosomes, or synapsed with themselves by folding in half. Additionally, without PP4 activity, the number of DNA breaks and of crossover recombination events were both independently reduced. The latter two defects became even worse with increasing age, indicating that older animals require PP4 to a greater extent. These findings shed light on how protein phosphorylation controls meiotic events, and demonstrate unanticipated, important roles for PP4.

Vyšlo v časopise: Protein Phosphatase 4 Promotes Chromosome Pairing and Synapsis, and Contributes to Maintaining Crossover Competence with Increasing Age. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004638
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004638

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