Rice is a monocotyledonous plant that is distinct from the dicotyledonous model plant Arabidopsis in many aspects. In Arabidopsis, ethylene-induced root inhibition is independent of ABA action. In rice, however, we report here that ethylene inhibition of root growth requires ABA function. We identified MHZ4, a rice homolog of Arabidopsis ABA4 that is involved in ABA biosynthesis. The mhz4 mutant displayed reduced ABA level and exhibited ethylene-hyposensitive root, but -hypersensitive coleoptile phenotypes in etiolated seedlings. Exogenous application of ABA largely recovered the defective ethylene responses. Overexpression of MHZ4 resulted in enhanced and reduced ethylene response in the roots and coleoptiles, respectively. In root, MHZ4-dependent ABA pathway genetically acts at or downstream of ethylene receptors and positively regulates root ethylene response. Moreover, ethylene treatment stimulated ABA production in roots at least through transcriptional activation of MHZ4. The results indicate that ethylene-induced root inhibition in rice is largely mediated through MHZ4-dependent ABA function. In coleoptile, MHZ4-dependent ABA pathway acts at or upstream of OsEIN2 and negatively regulates coleoptile ethylene response, possibly via transcriptional suppression of OsEIN2. Together, our findings reveal a novel mode of ethylene-ABA interaction which is fundamentally different from that in Arabidopsis.
Vyšlo v časopise: Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice ( L.) Seedlings. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004701 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004701
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