The bHLH-PAS Transcription Factor Dysfusion Regulates Tarsal Joint Formation in Response to Notch Activity during Leg Development

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One of the keys of the evolutionary success of arthropods, the most diversified group of animals, is the acquisition of joints that allow the articulation of their appendages. Two main kinds of joints with different morphologies and evolutionary origin are found in the fly leg: the proximal or “true” joints that are motile due to muscular attachment and the distal joints that are immotile. A common event during joint formation is the activation of the Notch pathway at the presumptive joints along the leg proximo-distal axis. In this work we investigated how the same pathway, Notch, can control the formation of such homologous although different structures. We described that the transcription factor Dysfusion (Dys) is a Notch target required for distal joint development and that is sufficient to induce joint-like structures when ectopically expressed. Dys controls two important morphogenetic events that direct tarsal joint development such as programed cell death and epithelial cell shape. Moreover, we identified a regulatory DNA sequence that controls dys expression in the tarsal segment by direct binding of the transcriptional effector of the Notch pathway Su(H). Thus, Notch controls the development of proximal vs distal joints by the recruitment of specific downstream target genes such as dys.

Vyšlo v časopise: The bHLH-PAS Transcription Factor Dysfusion Regulates Tarsal Joint Formation in Response to Notch Activity during Leg Development. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004621
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004621

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