Genetic Influences on Translation in Yeast

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Individuals in a species differ from each other in many ways. For many traits, a fraction of this variation is genetic—it is caused by DNA sequence variants in the genome of each individual. Some of these variants influence traits by altering how much certain genes are expressed, i.e. how many mRNA and protein molecules are made in different individuals. Surprisingly, earlier work has found that the effects of genetic variants on mRNA and protein levels for the same genes appear to be very different. Many variants appeared to influence only mRNA (but not protein) levels, and vice versa. In this paper, we studied this question by using a technique called “ribosome profiling” to measure translation (the cellular process of reading mRNA molecules and synthesizing protein molecules) in two yeast strains. We found that the genetic differences between these two strains influence translation for hundreds of genes. Because most of these effects were small in magnitude, they explain at most a small fraction of the discrepancies between the effects of genetic variants on mRNA and protein levels.

Vyšlo v časopise: Genetic Influences on Translation in Yeast. PLoS Genet 10(10): e32767. doi:10.1371/journal.pgen.1004692
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004692

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