MDRL lncRNA Regulates the Processing of miR-484 Primary Transcript by Targeting miR-361

English version České info

Long non-coding RNAs (lncRNAs) have been shown to be involved in a wide range of biological functions. However, studies linking individual lncRNA to the mitochondrial fission program remain scarce. Also, it remains unknown whether lncRNAs can operate in the processing of miRNA primary transcript. Here, we provide causal evidence for the involvement of the lncRNA MDRL in the mitochondrial dynamics and the processing of miR-484 primary transcript in cardiomyocyte. We identified MDRL which can act as an endogenous ‘sponge’ that directly binds to miR-361 and downregulates its expression levels. miR-361 can directly bind to primary transcript of miR-484 and prevent its processing by Drosha into pre-miR-484. MDRL inhibits mitochondrial fission and apoptosis by miR-361 and miR-484. Our present study reveals a novel regulating model which is composed of MDRL, miR-361 and miR-484. Modulation of their levels may provide a new approach for tackling myocardial infarction.

Vyšlo v časopise: MDRL lncRNA Regulates the Processing of miR-484 Primary Transcript by Targeting miR-361. PLoS Genet 10(7): e32767. doi:10.1371/journal.pgen.1004467
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004467

Souhrn

Zdroje

1. GongC, MaquatLE (2011) lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3 [prime] UTRs via Alu elements. Nature 470 : 284–288.

2. GoodrichJA, KugelJF (2006) Non-coding-RNA regulators of RNA polymerase II transcription. Nature Reviews Molecular Cell Biology 7 : 612–616.

3. EisPS, TamW, SunL, ChadburnA, LiZ, et al. (2005) Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proceedings of the National Academy of Sciences of the United States of America 102 : 3627–3632.

4. KhaitanD, DingerME, MazarJ, CrawfordJ, SmithMA, et al. (2011) The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apoptosis and invasion. Cancer research 71 : 3852–3862.

5. GingerMR, ShoreAN, ContrerasA, RijnkelsM, MillerJ, et al. (2006) A noncoding RNA is a potential marker of cell fate during mammary gland development. Proceedings of the National Academy of Sciences 103 : 5781–5786.

6. Kanduri C. Kcnq1ot1: a chromatin regulatory RNA; 2011. Elsevier. pp. 343–350.

7. BeltranM, PuigI, PeñaC, GarcíaJM, ÁlvarezAB, et al. (2008) A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition. Genes & development 22 : 756–769.

8. TripathiV, EllisJD, ShenZ, SongDY, PanQ, et al. (2010) The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Molecular cell 39 : 925–938.

9. CesanaM, CacchiarelliD, LegniniI, SantiniT, SthandierO, et al. (2011) A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell 147 : 358–369.

10. HwangH-W, WentzelEA, MendellJT (2007) A hexanucleotide element directs microRNA nuclear import. Science 315 : 97–100.

11. ZisoulisDG, KaiZS, ChangRK, PasquinelliAE (2012) Autoregulation of microRNA biogenesis by let-7 and Argonaute. Nature 486 : 541–544.

12. TanakaA, YouleRJ (2008) A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis. Molecular cell 29 : 409–410.

13. DelettreC, LenaersG, GriffoinJ-M, GigarelN, LorenzoC, et al. (2000) Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy. Nature genetics 26 : 207–210.

14. MarzettiE, CalvaniR, CesariM, BufordTW, LorenziM, et al. (2013) Mitochondrial dysfunction and sarcopenia of aging: from signaling pathways to clinical trials. The international journal of biochemistry & cell biology 45 : 2288–301 doi:10.1016/j.biocel.2013.06.024

15. SuB, WangX, ZhengL, PerryG, SmithMA, et al. (2010) Abnormal mitochondrial dynamics and neurodegenerative diseases. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1802 : 135–142.

16. WangW, LiL, LinW-L, DicksonDW, PetrucelliL, et al. (2013) The ALS disease-associated mutant TDP-43 impairs mitochondrial dynamics and function in motor neurons. Human molecular genetics 22 doi:10.1093/hmg/ddt1319

17. ZüchnerS, MersiyanovaIV, MugliaM, Bissar-TadmouriN, RochelleJ, et al. (2004) Mutations in the mitochondrial GTPase mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A. Nature genetics 36 : 449–451.

18. BreckenridgeDG, StojanovicM, MarcellusRC, ShoreGC (2003) Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol. The Journal of cell biology 160 : 1115–1127.

19. FrankS, GaumeB, Bergmann-LeitnerES, LeitnerWW, RobertEG, et al. (2001) The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Developmental cell 1 : 515–525.

20. GriffinEE, GraumannJ, ChanDC (2005) The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondria. The Journal of cell biology 170 : 237–248.

21. McBrideHM, NeuspielM, WasiakS (2006) Mitochondria: more than just a powerhouse. Current Biology 16: R551–R560.

22. SuenD-F, NorrisKL, YouleRJ (2008) Mitochondrial dynamics and apoptosis. Genes & development 22 : 1577–1590.

23. WasiakS, ZuninoR, McBrideHM (2007) Bax/Bak promote sumoylation of DRP1 and its stable association with mitochondria during apoptotic cell death. The Journal of cell biology 177 : 439–450.

24. WangK, LongB, JiaoJ-Q, WangJ-X, LiuJ-P, et al. (2012) miR-484 regulates mitochondrial network through targeting Fis1. Nature communications 3 : 781.

25. CazallaD, YarioT, SteitzJA (2010) Down-regulation of a host microRNA by a Herpesvirus saimiri noncoding RNA. Science 328 : 1563–1566.

26. WangJ, LiuX, WuH, NiP, GuZ, et al. (2010) CREB up-regulates long non-coding RNA, HULC expression through interaction with microRNA-372 in liver cancer. Nucleic acids research 38 : 5366–5383.

27. KallenAN, ZhouXB, XuJ, QiaoC, MaJ, et al. (2013) The imprinted H19 lncRNA antagonizes let-7 microRNAs. Mol Cell 52 : 101–112.

28. ClemsonCM, HutchinsonJN, SaraSA, EnsmingerAW, FoxAH, et al. (2009) An Architectural Role for a Nuclear Noncoding RNA: NEAT1 RNA Is Essential for the Structure of Paraspeckles. Molecular cell 33 : 717–726.

29. HussJM, KellyDP (2005) Mitochondrial energy metabolism in heart failure: a question of balance. Journal of Clinical Investigation 115 : 547–555.

30. TanW-Q, WangK, LvD-Y, LiP-F (2008) Foxo3a inhibits cardiomyocyte hypertrophy through transactivating catalase. Journal of Biological Chemistry 283 : 29730–29739.