Wapl has been shown to play an integral role in the removal of cohesin from chromosomes during mitotic prophase. While Wapl's role appears to be conserved between yeast, fly and animal cells, structural and possible mechanistic differences have also been identified. As part of a study to better understand the protein and its role(s) we have characterized Wapl in plants. We show that Arabidopsis contains two copies of WAPL that share overlapping functions. Inactivation of the individual genes has no effect. Plants containing mutations in both genes growth normally but exhibit reduced fertility due to alterations in meiosis. Cohesin removal from chromosomes during meiotic prophase is blocked in wapl mutant plants resulting in unresolved bivalents and uneven chromosome segregation. In contrast, cohesin appears to be removed normally in mitotic cells. These results demonstrate that WAPL plays a critical role in removing cohesin from meiotic chromosomes. They also suggest that the mechanism involved in prophase removal of cohesin may vary between mitosis and meiosis in plants. Finally, wapl mutations suppress ctf7-associated lethality and restore normal growth and partial fertility to ctf7 mutant plants, suggesting that sister chromatid cohesion is not essential for plant growth and development.
Vyšlo v časopise: WAPL Is Essential for the Prophase Removal of Cohesin during Meiosis. PLoS Genet 10(7): e32767. doi:10.1371/journal.pgen.1004497 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004497
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