Phosphorylation of a WRKY Transcription Factor by MAPKs Is Required for Pollen Development and Function in

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Pollen development, or male gametogenesis, is a process by which a haploid uninucleate microspore undergoes cell division and specification to form a mature pollen grain containing two sperm cells. The highly defined cell linage makes pollen development an ideal model to understand the regulation of plant cellular development. Pollen development has multiple phases and involves dynamic changes in gene expression, which highlights the importance of transcription factors and their regulatory pathway(s). In this report, we demonstrate that WRKY34 and WRKY2, two closely related WRKY transcription factors in Arabidopsis, play important roles in pollen development. WRKY34 is phosphorylated by MPK3/MPK6, two functionally redundant mitogen-activated protein kinases (MAPKs or MPKs), at early stages in pollen development. Utilizing a combination of genetic, biochemical, and cytological tools, we determined that this MAPK-WRKY signaling module functions at the early stage of pollen development. Loss of function of this pathway reduces pollen viability, and the surviving pollen has poor germination and reduced pollen tube growth, all of which reduce the transmission rate of the mutant pollen. This study discovers a novel stage-specific signaling pathway in pollen development.

Vyšlo v časopise: Phosphorylation of a WRKY Transcription Factor by MAPKs Is Required for Pollen Development and Function in. PLoS Genet 10(5): e32767. doi:10.1371/journal.pgen.1004384
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004384

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