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preLekára.sk / Odborné časopisy / PLOS Genetics / 2014 - 5

Heterogeneity in the Frequency and Characteristics of Homologous Recombination in Pneumococcal Evolution

English version České info

Streptococcus pneumoniae, a bacterium commonly carried asymptomatically by children, is a major cause of diseases such as pneumonia and meningitis. The species is genetically diverse and is known to frequently undergo the remarkable process of transformation via homologous recombination. In this process, the bacterial cell incorporates DNA from other, closely related bacteria into its own genome, which can result in the development of antibiotic resistance or allow cells to evade vaccines. Therefore it is important to quantify the impact of this process on the evolution of S. pneumoniae to understand how quickly the species can respond to the introduction of such clinical interventions. In this study we followed the recombination process by studying the evolution of two important and very different lineages of S. pneumoniae, PMEN1 and CC180, using newly available population genomic data. We found that pneumococcus evolves via two distinct processes that we term micro -⁠ and macro-recombination. Micro-recombination led to acquisition of single, short DNA fragments, while macro-recombination tended to incorporate multiple, long DNA fragments. Interestingly, macro-recombination was associated with major phenotypic changes. We argue that greater insight into the adaptive role of recombination in pneumococcus requires a good understanding of both rates of homologous recombination and population dynamics of the bacterium in natural populations.

Vyšlo v časopise: Heterogeneity in the Frequency and Characteristics of Homologous Recombination in Pneumococcal Evolution. PLoS Genet 10(5): e32767. doi:10.1371/journal.pgen.1004300
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004300

Souhrn

Streptococcus pneumoniae, a bacterium commonly carried asymptomatically by children, is a major cause of diseases such as pneumonia and meningitis. The species is genetically diverse and is known to frequently undergo the remarkable process of transformation via homologous recombination. In this process, the bacterial cell incorporates DNA from other, closely related bacteria into its own genome, which can result in the development of antibiotic resistance or allow cells to evade vaccines. Therefore it is important to quantify the impact of this process on the evolution of S. pneumoniae to understand how quickly the species can respond to the introduction of such clinical interventions. In this study we followed the recombination process by studying the evolution of two important and very different lineages of S. pneumoniae, PMEN1 and CC180, using newly available population genomic data. We found that pneumococcus evolves via two distinct processes that we term micro -⁠ and macro-recombination. Micro-recombination led to acquisition of single, short DNA fragments, while macro-recombination tended to incorporate multiple, long DNA fragments. Interestingly, macro-recombination was associated with major phenotypic changes. We argue that greater insight into the adaptive role of recombination in pneumococcus requires a good understanding of both rates of homologous recombination and population dynamics of the bacterium in natural populations.

Zdroje

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