Protein Composition of Infectious Spores Reveals Novel Sexual Development and Germination Factors in

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Spores are a critical cell type that allow long-term survival of diverse organisms from bacteria to fungi to plants. Among fungi, spores are often formed when growth conditions are poor; spores can then disperse to more favorable environments and reinitiate growth. Spores of some environmental fungi can cause fatal disease in humans. Here we used the meningitis-causing yeast Cryptococcus neoformans to determine the roles of spore-enriched proteins in spore biology. Using a combined proteomics-genetics approach, we identified eighteen spore-enriched proteins, knocked out the genes encoding each of them, and assessed the resulting strains for phenotypes in a broad array of assays. We predicted that mutant strains would be likely to show defects in spore-specific processes, but instead, we discovered that the majority harbored defects in sexual development, the process by which spores are formed. Only one mutant exhibited a defect in a spore-specific process (germination). Our data reveal that many spore-represented proteins are associated with pre-spore developmental processes, rather than intrinsic spore-specific properties or processes. These findings indicate a previously unknown molecular connection between the developmental process that results in spore biogenesis and the composition of infectious spores.

Vyšlo v časopise: Protein Composition of Infectious Spores Reveals Novel Sexual Development and Germination Factors in. PLoS Genet 11(8): e32767. doi:10.1371/journal.pgen.1005490
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005490

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