How signalling pathways regulate the formation of organs with a precise size and pattern of differentiation is a fundamental question in developmental genetics. One classical example of the link between signalling and organ development is the regulation of wing disc development by the Decapentaplegic/BMP (Dpp) signalling pathway in Drosophila. A key outcome of this pathway is the transcriptional activation of the spalt major (salm) and spalt related (salr) genes, both encoding transcription factors. In this manner, the identification of Salm/Salr target genes is a critical step towards the understanding of the mode of action of these proteins and the genetic logic underlying the regulation of wing development by the Dpp signalling pathway. In order to identify these target genes, we used expression microarrays, in situ hybridization and phenotypic analysis. We identified an unexpected complexity in the transcriptional landscape of the wing disc that includes genes positively and negatively regulated by Salm/Salr. These findings have major implications for the reconstruction of the genetic hierarchy initiated by the Dpp pathway and leading to the formation of a wing with a correct size and pattern, because some of the genes we identified could explain particular aspects of the sal mutant phenotype.
Vyšlo v časopise: The Spalt Transcription Factors Generate the Transcriptional Landscape of the Wing Pouch Central Region. PLoS Genet 11(8): e32767. doi:10.1371/journal.pgen.1005370 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005370
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