SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation

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Mitochondria provide most of the energy required for key metabolic and cellular processes that are essential for life. The biogenesis of the mitochondrial oxidative phosphorylation system, the site of energy conversion, is dependent on the coordinated expression of the mitochondrial and nuclear genomes. Mitochondrial gene expression is largely regulated at the post-transcriptional level by RNA-binding proteins, including the LRPPRC-SLIRP complex. It is still unclear how the proteins within this complex regulate mitochondrial RNA metabolism. Here, we have knocked out the Slirp gene in mice to dissect the individual roles of LRPPRC and SLIRP and provide further insights into the mechanisms governing post-transcriptional regulation of mitochondrial gene expression. LRPPRC is required for the maintenance of mitochondrial mRNA polyadenylation whereas SLIRP, by facilitating the presentation (or association) of mRNAs to the mitochondrial ribosome, regulates the rate of translation. In addition, we demonstrate that mitochondrial mRNAs in mammals are present in quantities that far exceed those needed to maintain normal physiology under basal conditions.

Vyšlo v časopise: SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation. PLoS Genet 11(8): e32767. doi:10.1371/journal.pgen.1005423
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005423

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