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The Small Protein MntS and Exporter MntP Optimize the Intracellular Concentration of Manganese

English version České info

The model organism Escherichia coli can employ manganese to activate a variety of enzymes, but it does so only when iron is unavailable or the cell is assaulted by oxidants. Under those stress conditions, E. coli activates the synthesis of its MntH manganese importer. However, manganese can also be toxic, and over-accumulation of the metal must be avoided. We investigated additional mechanisms by which the cell ensures that its intracellular manganese pool is sufficient but not excessive. MntS was identified as a small protein that is synthesized when manganese levels decline; it helps to enlarge the manganese pool. In contrast, MntP is a manganese exporter that is synthesized when intracellular manganese levels rise; it keeps manganese levels from becoming too high. In manganese-rich medium either the forced expression of MntS or the absence of MntP led to manganese toxicity, suggesting the possibility that MntS operates as an inhibitor of MntP. This toxicity occurs because excess manganese inhibits the synthesis of heme, a cofactor that is essential for aerobic growth. Thus E. coli controls manganese levels by balancing the actions of MntH, MntP, and MntS against one another. This arrangement allows cells to adapt to shifts in manganese availability and demand.

Vyšlo v časopise: The Small Protein MntS and Exporter MntP Optimize the Intracellular Concentration of Manganese. PLoS Genet 11(3): e32767. doi:10.1371/journal.pgen.1004977
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004977

Souhrn

The model organism Escherichia coli can employ manganese to activate a variety of enzymes, but it does so only when iron is unavailable or the cell is assaulted by oxidants. Under those stress conditions, E. coli activates the synthesis of its MntH manganese importer. However, manganese can also be toxic, and over-accumulation of the metal must be avoided. We investigated additional mechanisms by which the cell ensures that its intracellular manganese pool is sufficient but not excessive. MntS was identified as a small protein that is synthesized when manganese levels decline; it helps to enlarge the manganese pool. In contrast, MntP is a manganese exporter that is synthesized when intracellular manganese levels rise; it keeps manganese levels from becoming too high. In manganese-rich medium either the forced expression of MntS or the absence of MntP led to manganese toxicity, suggesting the possibility that MntS operates as an inhibitor of MntP. This toxicity occurs because excess manganese inhibits the synthesis of heme, a cofactor that is essential for aerobic growth. Thus E. coli controls manganese levels by balancing the actions of MntH, MntP, and MntS against one another. This arrangement allows cells to adapt to shifts in manganese availability and demand.

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Článek Linking Aβ42-Induced Hyperexcitability to Neurodegeneration, Learning and Motor Deficits, and a Shorter Lifespan in an Alzheimer’s Model
Článek The Complex Contributions of Genetics and Nutrition to Immunity in
Článek NatB Domain-Containing CRA-1 Antagonizes Hydrolase ACER-1 Linking Acetyl-CoA Metabolism to the Initiation of Recombination during . Meiosis
Článek Transcriptomic Profiling of Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs
Článek Osteopetrorickets due to Snx10 Deficiency in Mice Results from Both Failed Osteoclast Activity and Loss of Gastric Acid-Dependent Calcium Absorption
Článek A Genomic Portrait of Haplotype Diversity and Signatures of Selection in Indigenous Southern African Populations
Článek Sequence Features and Transcriptional Stalling within Centromere DNA Promote Establishment of CENP-A Chromatin
Článek Inhibits Neuromuscular Junction Growth by Downregulating the BMP Receptor Thickveins
Článek Replicative DNA Polymerase δ but Not ε Proofreads Errors in and in
Článek Unsaturation of Very-Long-Chain Ceramides Protects Plant from Hypoxia-Induced Damages by Modulating Ethylene Signaling in
Článek A Meta-analysis of Gene Expression Signatures of Blood Pressure and Hypertension
Článek Pervasive Variation of Transcription Factor Orthologs Contributes to Regulatory Network Evolution
Článek Network Analyses Reveal Novel Aspects of ALS Pathogenesis
Článek A Role for the Budding Yeast Separase, Esp1, in Ty1 Element Retrotransposition
Článek Nab3 Facilitates the Function of the TRAMP Complex in RNA Processing via Recruitment of Rrp6 Independent of Nrd1
Článek A RecA Protein Surface Required for Activation of DNA Polymerase V
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PLOS Genetics

2015 Číslo 3
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Autori: doc. MUDr. David Zemánek, Ph.D., MUDr. Anna Chaloupka, Ph.D.
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