PHABULOSA Controls the Quiescent Center-Independent Root Meristem Activities in


Plant roots are programmed to grow continuously into the soil, searching for nutrients and water. The iterative process of cell division, elongation, and differentiation contributes to root growth. The quiescent center (QC) is known to maintain the root meristem, and thus ensure root growth. In this study, we report a novel aspect of root growth regulation controlled independently of the QC by PHABULOSA (PHB). In shr mutant plants, PHB, which in the meristem is actively restricted to the central region of the stele by SHORTROOT (SHR) via miR165/6, suppresses root meristem activity leading to root growth arrest. A high concentration of PHB in the stele does this by modulating B-ARR activity through a QC-independent pathway. Accordingly, we observed a significant recovery of root meristem activity and growth in the shr phb double mutant, while the QC remained absent. However, the presence of QC may be required to sustain continuous root growth. On the basis of our results, we propose that SHR maintains root growth via two separate pathways: by modulating PHB levels in the root stele, and by maintaining the QC identity.


Vyšlo v časopise: PHABULOSA Controls the Quiescent Center-Independent Root Meristem Activities in. PLoS Genet 11(3): e32767. doi:10.1371/journal.pgen.1004973
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pgen.1004973

Souhrn

Plant roots are programmed to grow continuously into the soil, searching for nutrients and water. The iterative process of cell division, elongation, and differentiation contributes to root growth. The quiescent center (QC) is known to maintain the root meristem, and thus ensure root growth. In this study, we report a novel aspect of root growth regulation controlled independently of the QC by PHABULOSA (PHB). In shr mutant plants, PHB, which in the meristem is actively restricted to the central region of the stele by SHORTROOT (SHR) via miR165/6, suppresses root meristem activity leading to root growth arrest. A high concentration of PHB in the stele does this by modulating B-ARR activity through a QC-independent pathway. Accordingly, we observed a significant recovery of root meristem activity and growth in the shr phb double mutant, while the QC remained absent. However, the presence of QC may be required to sustain continuous root growth. On the basis of our results, we propose that SHR maintains root growth via two separate pathways: by modulating PHB levels in the root stele, and by maintaining the QC identity.


Zdroje

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