Rapid Evolution of Recombinant for Xylose Fermentation through Formation of Extra-chromosomal Circular DNA

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Xylose is an important component of lignocellulose hydrolysates used for the production of bioethanol, but the yeast Saccharomyces cerevisiae is unable to utilize xylose. Insertion of a bacterial xylose isomerase gene and improvement of growth on xylose by evolutionary adaptation resulted in amplification of this gene and efficient xylose fermentation capacity. Further analysis of the final and intermediate strains from the evolutionary adaptation process revealed interesting features about the mechanisms involved in gene amplification events, which have occurred frequently in natural evolution. We now show that a circular DNA element was spontaneously created by the yeast, encompassing the xylose isomerase gene and an ARS element, present by coincidence adjacent of the inserted xylose isomerase gene. ARS elements are the sites where DNA polymerase initiates duplication of DNA. Interestingly, this has revealed for the first time in yeast that circular DNA plasmids can be created from genomic DNA in the absence of flanking repetitive sequences.

Vyšlo v časopise: Rapid Evolution of Recombinant for Xylose Fermentation through Formation of Extra-chromosomal Circular DNA. PLoS Genet 11(3): e32767. doi:10.1371/journal.pgen.1005010
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005010

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