A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Ovulation

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Ovulation is the process of releasing fertilizable oocytes from the ovary and is essential for metazoan reproduction. Our recent work has demonstrated principles governing ovulation process that are highly conserved across species, such that both mammals and Drosophila utilize matrix metalloproteinase (Mmp) to degrade extracellular matrix and weaken the follicle wall for follicle rupture. However, a fundamental question remaining in the field is how Mmp activity is precisely regulated during ovulation. This paper reports that Drosophila octopamine (OA), the insect equivalent of norepinephrine (NE), is the signal to induce Mmp activity through activating its receptor Oamb on mature follicle cells and that this may induce ovulation. These findings allow us to develop the first ex vivo follicle rupture assay for Drosophila, which gives us unprecedented ability to characterize the entire follicle rupturing process ex vivo and to identify essential factors for ovulation. Furthermore, we show that NE partially fulfills OA’s role in inducing follicle rupture ex vivo, indicating that follicular adrenergic signal is a conserved signal to regulating Mmp activity and ovulation. Our work not only sheds light on the long-standing question of Mmp regulation, but also may lead to a better understanding of Mmp and NE linked pathological processes including cancer metastasis and polycystic ovary syndrome.

Vyšlo v časopise: A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Ovulation. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005604
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005604

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