Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation

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Ribonucleic acids (RNA) are key intermediates between the heritable genome and the expression of proteins. Thus the level of a specific RNA determines the capability of the cell to produce the corresponding protein. Bacteria maintain a very high turnover (high production but also high decay-rate) of RNA molecules, to ensure that they can, at any time, change the protein production program in response to outside stimuli. However, all types of RNA molecules do not have the same half-life, some last for tens of minutes whereas most only last for seconds. Here we examine how endoribonuclease Y (RNase Y), one of the enzymes that cleave RNA into smaller fragments, chooses its targets from the pool of cellular RNA. We show that RNase Y prefers to cleave RNA at a specific ribonucleotide sequence, thus keeping the RNA degradation to the correct level, not too fast and not too slow. RNase Y is anchored to the inside of the cell envelope, and we furthermore show that releasing RNase Y into the interior of the cell results in poor growth, but is at the same time able to compensate for the loss of CshA, another factor in the RNA decay machinery.

Vyšlo v časopise: Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005577
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005577

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