Oriented Cell Division in the . Embryo Is Coordinated by G-Protein Signaling Dependent on the Adhesion GPCR LAT-1

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During embryogenesis an entire organism develops from a single cell. This process is vital for the formation of life, thus cell division occurs with a very distinct orientation and pattern that is tightly controlled by several signaling pathways. The mechanisms underlying these pathways are complex and not yet fully understood. In the roundworm Caenorhabditis elegans, a common genetic model, the patterns and orientations in which cells divide in the embryo have been well characterized offering an ideal model to study the molecular mechanisms involved. Here, we show that the signal mediated by the adhesion G protein-coupled receptor LAT-1 is based on cAMP. This second messenger is essential for the orientation of distinct cell division planes in the early embryo. Studies based on a lat-1 knockout mutant reveal that LAT-1 signaling affects the levels of the second messenger cAMP in the cells via a specific G protein. Thereby the receptor is activated by an intrinsic sequence. This pathway is the first one clearly shown to involve a G protein-coupled receptor-dependent G-protein signal in orientation of embryonic cell division, offering a novel level of regulation of this process among other described pathways.

Vyšlo v časopise: Oriented Cell Division in the . Embryo Is Coordinated by G-Protein Signaling Dependent on the Adhesion GPCR LAT-1. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005624
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005624

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