Single Strand Annealing Plays a Major Role in RecA-Independent Recombination between Repeated Sequences in the Radioresistant Bacterium


Deinococcus radiodurans is known for its exceptional ability to tolerate exposure to DNA damaging agents and, in particular, to very high doses of ionizing radiation. This exceptional radioresistance results from many features including efficient DNA double strand break repair. Here, we examine genome stability in D. radiodurans before and after exposure to ionizing radiation. Rearrangements between repeated sequences are a major source of genome instability and can be deleterious to the organism. Thus, we measured the frequency of recombination between direct repeats separated by intervening sequences of various lengths in the presence or absence of radiation-induced DNA double strand breaks. Strikingly, we showed that the frequency of deletions was as high in strains devoid of the RecA, RecF or RecO proteins as in wild type bacteria, suggesting a very efficient RecA-independent process able to generate genome rearrangements. Our results suggest that single strand annealing may play a major role in genome instability in the absence of homologous recombination.


Vyšlo v časopise: Single Strand Annealing Plays a Major Role in RecA-Independent Recombination between Repeated Sequences in the Radioresistant Bacterium. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005636
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pgen.1005636

Souhrn

Deinococcus radiodurans is known for its exceptional ability to tolerate exposure to DNA damaging agents and, in particular, to very high doses of ionizing radiation. This exceptional radioresistance results from many features including efficient DNA double strand break repair. Here, we examine genome stability in D. radiodurans before and after exposure to ionizing radiation. Rearrangements between repeated sequences are a major source of genome instability and can be deleterious to the organism. Thus, we measured the frequency of recombination between direct repeats separated by intervening sequences of various lengths in the presence or absence of radiation-induced DNA double strand breaks. Strikingly, we showed that the frequency of deletions was as high in strains devoid of the RecA, RecF or RecO proteins as in wild type bacteria, suggesting a very efficient RecA-independent process able to generate genome rearrangements. Our results suggest that single strand annealing may play a major role in genome instability in the absence of homologous recombination.


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