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Intermediate Levels of CodY Activity Are Required for Derepression of the Branched-Chain Amino Acid Permease, BraB

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Expression of Bacillus subtilis BraB, a branched-chain amino acid permease, is under both negative and positive control by a global transcriptional regulator CodY. The negative control is direct and the positive control is indirect and mediated by another B. subtilis pleiotropic transcriptional regulator, ScoC, which, in turn, is repressed by CodY. Thus, CodY and ScoC form a feed-forward regulatory loop at the braB promoter. In a very unusual manner, the interaction of CodY and ScoC results in high braB expression only at intermediate CodY activities; braB expression remains low both at high and low CodY activities. The novel regulation of braB shows that important, novel regulatory phenomena can be missed by analyzing null mutants in regulatory genes but revealed by using mutants with partial activity.

Vyšlo v časopise: Intermediate Levels of CodY Activity Are Required for Derepression of the Branched-Chain Amino Acid Permease, BraB. PLoS Genet 11(10): e32767. doi:10.1371/journal.pgen.1005600
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005600

Souhrn

Expression of Bacillus subtilis BraB, a branched-chain amino acid permease, is under both negative and positive control by a global transcriptional regulator CodY. The negative control is direct and the positive control is indirect and mediated by another B. subtilis pleiotropic transcriptional regulator, ScoC, which, in turn, is repressed by CodY. Thus, CodY and ScoC form a feed-forward regulatory loop at the braB promoter. In a very unusual manner, the interaction of CodY and ScoC results in high braB expression only at intermediate CodY activities; braB expression remains low both at high and low CodY activities. The novel regulation of braB shows that important, novel regulatory phenomena can be missed by analyzing null mutants in regulatory genes but revealed by using mutants with partial activity.

Zdroje

1. Belitsky BR. Role of branched-chain amino acid transport in Bacillus subtilis CodY activity. J Bacteriol. 2015;197(8):1330–8. doi: 10.1128/JB.02563-14 25645558

2. Belitsky BR, Sonenshein AL. Contributions of multiple binding sites and effector-independent binding to CodY-mediated regulation in Bacillus subtilis. J Bacteriol. 2011;193(2):473–84. doi: 10.1128/JB.01151-10 21097623

3. Sonenshein AL. CodY, a global regulator of stationary phase and virulence in Gram-positive bacteria. Curr Opin Microbiol. 2005;8(2):203–7. 15802253

4. Sonenshein AL. Control of key metabolic intersections in Bacillus subtilis. Nat Rev Microbiol. 2007;5(12):917–27. 17982469

5. Belitsky BR, Gustafsson MC, Sonenshein AL, Von Wachenfeldt C. An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport. J Bacteriol. 1997;179(17):5448–57. 9287000

6. Molle V, Nakaura Y, Shivers RP, Yamaguchi H, Losick R, Fujita Y, et al. Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis. J Bacteriol. 2003;185(6):1911–22. 12618455

7. Belitsky BR, Sonenshein AL. Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution. Proc Natl Acad Sci U S A. 2013;110(17):7026–31. doi: 10.1073/pnas.1300428110 23569278

8. Brinsmade SR, Alexander EL, Livny J, Stettner AI, Segre D, Rhee KY, et al. Hierarchical expression of genes controlled by the Bacillus subtilis global regulatory protein CodY. Proc Natl Acad Sci USA. 2014;111(22):8227–32. doi: 10.1073/pnas.1321308111 24843172

9. Guedon E, Serror P, Ehrlich SD, Renault P, Delorme C. Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis. Mol Microbiol. 2001;40(5):1227–39. 11401725

10. Petranovic D, Guedon E, Sperandio B, Delorme C, Ehrlich D, Renault P. Intracellular effectors regulating the activity of the Lactococcus lactis CodY pleiotropic transcription regulator. Mol Microbiol. 2004;53(2):613–21. 15228538

11. Shivers RP, Sonenshein AL. Activation of the Bacillus subtilis global regulator CodY by direct interaction with branched-chain amino acids. Mol Microbiol. 2004;53(2):599–611. 15228537

12. Ratnayake-Lecamwasam M, Serror P, Wong KW, Sonenshein AL. Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels. Genes Dev. 2001;15(9):1093–103. 11331605

13. Handke LD, Shivers RP, Sonenshein AL. Interaction of Bacillus subtilis CodY with GTP. J Bacteriol. 2008;190(3):798–806. 17993518

14. Brinsmade SR, Sonenshein AL. Dissecting complex metabolic integration provides direct genetic evidence for CodY activation by guanine nucleotides. J Bacteriol. 2011;193(20):5637–48. doi: 10.1128/JB.05510-11 21856856

15. Caldwell R, Sapolsky R, Weyler W, Maile RR, Causey SC, Ferrari E. Correlation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis. J Bacteriol. 2001;183(24):7329–40. 11717292

16. Dod B, Balassa G, Raulet E, Jeannoda V. Spore control (sco) mutations in Bacillus subtilis. II. Sporulation and the production of extracellular protease and α-amylase by scoC mutants. Molec Gen Genet. 1978;163 : 45–56.

17. Higerd TB, Hoch JA, Spizizen J. Hyperprotease-producing mutants of Bacillus subtilis. J Bacteriol. 1972;112(2):1026–8. 4628743

18. Kallio PT, Fagelson JE, Hoch JA, Strauch MA. The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein. J Biol Chem. 1991;266(20):13411–7. 1906467

19. Belitsky BR, Barbieri G, Albertini AM, Ferrari E, Strauch MA, Sonenshein AL. Interactive Regulation by the Bacillus subtilis Global Regulators CodY and ScoC. Mol Microbiol. 2015;97(4):698–716. doi: 10.1111/mmi.13056 25966844

20. Mangan S, Alon U. Structure and function of the feed-forward loop network motif. Proc Natl Acad Sci USA. 2003;100(21):11980–5. 14530388

21. Alon U. Network motifs: theory and experimental approaches. Nat Rev Genet. 2007;8(6):450–61. 17510665

22. den Hengst CD, van Hijum SA, Geurts JM, Nauta A, Kok J, Kuipers OP. The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element. J Biol Chem. 2005;280(40):34332–42. 16040604

23. Guedon E, Sperandio B, Pons N, Ehrlich SD, Renault P. Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in Firmicutes. Microbiology. 2005;151(Pt 12):3895–909. 16339935

24. Belitsky BR, Sonenshein AL. Genetic and biochemical analysis of CodY-binding sites in Bacillus subtilis. J Bacteriol. 2008;190(4):1224–36. 18083814

25. Belitsky BR, Sonenshein AL. Roadblock repression of transcription by Bacillus subtilis CodY. J Mol Biol. 2011;411(4):729–43. doi: 10.1016/j.jmb.2011.06.012 21699902

26. Belitsky BR, Sonenshein AL. CodY-mediated regulation of guanosine uptake in Bacillus subtilis. J Bacteriol. 2011;193(22):6276–87. doi: 10.1128/JB.05899-11 21926227

27. Slack FJ, Mueller JP, Sonenshein AL. Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon. J Bacteriol. 1993;175(15):4605–14. 8335620

28. Villapakkam AC, Handke LD, Belitsky BR, Levdikov VM, Wilkinson AJ, Sonenshein AL. Genetic and biochemical analysis of the interaction of Bacillus subtilis CodY with branched-chain amino acids. J Bacteriol. 2009;191(22):6865–76. doi: 10.1128/JB.00818-09 19749041

29. Serror P, Sonenshein AL. CodY is required for nutritional repression of Bacillus subtilis genetic competence. J Bacteriol. 1996;178(20):5910–5. 8830686

30. Smits WK, Hoa TT, Hamoen LW, Kuipers OP, Dubnau D. Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein. Mol Microbiol. 2007;64(2):368–81. 17493123

31. Koide A, Perego M, Hoch JA. ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis. J Bacteriol. 1999;181(13):4114–7. 10383984

32. Dod B, Balassa G. Spore control (sco) mutations in Bacillus subtilis. III. Regulation of extracellular protease synthesis in the spore control mutations scoC. Molec Gen Genet. 1978;163 : 57–63.

33. Perego M, Hoch JA. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis. J Bacteriol. 1988;170(6):2560–7. 3131303

34. Henner DJ, Ferrari E, Perego M, Hoch JA. Location of the targets of the hpr-97, sacU32(Hy), and sacQ36(Hy) mutations in upstream regions of the subtilisin promoter. J Bacteriol. 1988;170(1):296–300. 2447063

35. Ogura M, Matsuzawa A, Yoshikawa H, Tanaka T. Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE. J Bacteriol. 2004;186(10):3056–64. 15126467

36. Toma S, Del Bue M, Pirola A, Grandi G. nprR1 and nprR2 regulatory regions for neutral protease expression in Bacillus subtilis. J Bacteriol. 1986;167(2):740–3. 3090022

37. Derouiche A, Shi L, Bidnenko V, Ventroux M, Pigonneau N, Franz-Wachtel M, et al. Bacillus subtilis SalA is a phosphorylation-dependent transcription regulator that represses scoC and activates the production of the exoprotease AprE. Mol Microbiol. 2015;97(6):1195–208. doi: 10.1111/mmi.13098 26094643

38. Zeigler DR, Pragai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, et al. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008;190(21):6983–95. doi: 10.1128/JB.00722-08 18723616

39. Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–19. 2985470

40. Atkinson MR, Wray LV Jr., Fisher SH. Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis. J Bacteriol. 1990;172(9):4758–65. 2118500

41. Sambrook J, Fritsch EF, Maniatis TJ. Molecular cloning: a laboratory manual, 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 1989.

42. Daniel RA, Haiech J, Denizot F, Errington J. Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR. J Bacteriol. 1997;179(17):5636–8. 9287030

43. Guerout-Fleury AM, Shazand K, Frandsen N, Stragier P. Antibiotic-resistance cassettes for Bacillus subtilis. Gene. 1995;167(1–2):335–6. 8566804

44. Mueller JP, Bukusoglu G, Sonenshein AL. Transcriptional regulation of Bacillus subtilis glucose starvation-inducible genes: control of gsiA by the ComP-ComA signal transduction system. J Bacteriol. 1992;174(13):4361–73. 1378051

45. Berkmen MB, Lee CA, Loveday EK, Grossman AD. Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 2010;192(1):38–45. doi: 10.1128/JB.00860-09 19734305

46. Belitsky BR, Sonenshein AL. Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J Bacteriol. 1998;180(23):6298–305. 9829940

47. Barbieri G, Voigt B, Albrecht D, Hecker M, Albertini AM, Sonenshein AL, et al. CodY regulates expression of the Bacillus subtilis extracellular proteases Vpr and Mpr. J Bacteriol. 2015;197 : 1423–32. doi: 10.1128/JB.02588-14 25666135

48. Chary VK, Amaya EI, Piggot PJ. Neomycin -⁠ and spectinomycin-resistance replacement vectors for Bacillus subtilis. FEMS Microbiol Lett. 1997;153(1):135–9. 9252583

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