Molecular Insights Into the Evolutionary Pathway of O1 Atypical El Tor Variants

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In this report, we suggest a genetic mechanism of how the V. cholerae atypical El Tor variants were generated from classical and prototype El Tor biotype strains. An intermediary strain, containing the CTX-1 and CTX-2 prophages, was identified among the clinical isolates that were collected in 1991, when the atypical strains emerged. This strain can be converted into various Wave 2 atypical El Tor strains by eliminating prototype components, CTX-1 and RS1. Further, new types of the CTX phage genome can be generated from the intermediary strain by inter-chromosomal recombination between CTX phages and recombination between the CTX phage and RS1. These new CTX phages can be transduced into other El Tor strains, transforming them into Wave 3 atypical strains. This is a demonstrated instance of how a single-segment-genome CTX phage re-organizes its genome through recombination between different types of phage, leading to generation of new phage variants and atypical El Tor strains.

Vyšlo v časopise: Molecular Insights Into the Evolutionary Pathway of O1 Atypical El Tor Variants. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004384
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004384

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