Exploitation of Reporter Strains to Probe the Impact of Vaccination at Sites of Infection

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Mycobacterium tuberculosis (Mtb) remains a serious challenge to global health, a situation that is exacerbated by emerging drug resistance, the paucity of new antibiotics, and the absence of an effective vaccine. Whilst there is no doubt that an anti-tuberculosis vaccine would have an extraordinary impact on the control of this disease, it is not clear if such a goal is achievable. One major deficiency in our knowledge is an appreciation of how the same protective immune response that limits bacterial growth is also responsible for driving adaptation of Mtb towards successful maintenance of long-term infection. We have used our panel of reporter Mtb strains that fluoresce under different environmental stresses for experimental infections of naïve and vaccinated, wild type and immune-deficient, mouse strains. In addition, we utilized a novel replication reporter strain to visualize the distribution of replicating versus non-replicating bacteria within infected lung tissue. The data provide novel insights into how the host immune response impacts bacterial fitness, physiology, and growth rates during the course of infection in naïve and vaccinated mice.

Vyšlo v časopise: Exploitation of Reporter Strains to Probe the Impact of Vaccination at Sites of Infection. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004394
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004394

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