Multivalent Adhesion Molecule 7 Clusters Act as Signaling Platform for Host Cellular GTPase Activation and Facilitate Epithelial Barrier Dysfunction

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Vibrio parahaemolyticus is a bacterial pathogen which occurs in marine and estuarine environments. It is a main cause of gastrointestinal illness following the consumption of raw or undercooked seafood. In immunocompromised people, the bacteria can sometimes enter the bloodstream and cause septicemia, a serious and often fatal condition. V. parahaemolyticus attaches to host tissues using adhesive proteins. Multivalent Adhesion Molecule (MAM) 7 is an adhesin which helps the bacteria to hold onto the host cells early on during infection. It does so by binding two different molecules on the host, a protein (fibronectin) and phospholipids called phosphatidic acids. We show that MAM7 does not only play a role in sticking to host cells. By forming adhesin clusters on the host surface and binding to host lipids, it triggers signaling processes in the host. These include activation of RhoA, an important mediator of cytoskeletal dynamics. By doing so, MAM7 perturbs proteins at cellular junctions, which normally maintain the cells in the gut as a tightly sealed layer protective of environmental influences. When bacteria use MAM7 to attach to the intestine, the seals between cells break, permitting bacteria to cross the barrier and cause infection of underlying tissues.

Vyšlo v časopise: Multivalent Adhesion Molecule 7 Clusters Act as Signaling Platform for Host Cellular GTPase Activation and Facilitate Epithelial Barrier Dysfunction. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004421
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004421

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