A New Human 3D-Liver Model Unravels the Role of Galectins in Liver Infection by the Parasite


The study of liver infection is based on animal models, but the animal physiology does not always reflect the reality of the human host. This is particularly true for pathogens whose exclusive natural hosts are humans, such as Entamoeba histolytica, the protozoan parasite responsible for amoebiasis. Here, we constructed an experimental human 3D-liver model able to reproduce the first steps of amoebic hepatic infection (barrier crossing, tissue migration and pro-inflammatory reaction). Using this 3D-liver model we were able to decipher the first stages of hepatic invasion by E. histolytica and to unravel the role played by galectin-1 and galectin-3 during amoebic hepatic adhesion and pro-inflammatory reaction. Moreover, the model enables analysis usually not possible with in vivo samples, such as the quantification of pro-inflammatory cytokines released inside the tissue microenvironment. Our 3D-liver model has the potential to bridge the gap between animal models and the reality of the human host for the study of amoebic infection and other infectious diseases of the liver.


Vyšlo v časopise: A New Human 3D-Liver Model Unravels the Role of Galectins in Liver Infection by the Parasite. PLoS Pathog 10(9): e32767. doi:10.1371/journal.ppat.1004381
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.ppat.1004381

Souhrn

The study of liver infection is based on animal models, but the animal physiology does not always reflect the reality of the human host. This is particularly true for pathogens whose exclusive natural hosts are humans, such as Entamoeba histolytica, the protozoan parasite responsible for amoebiasis. Here, we constructed an experimental human 3D-liver model able to reproduce the first steps of amoebic hepatic infection (barrier crossing, tissue migration and pro-inflammatory reaction). Using this 3D-liver model we were able to decipher the first stages of hepatic invasion by E. histolytica and to unravel the role played by galectin-1 and galectin-3 during amoebic hepatic adhesion and pro-inflammatory reaction. Moreover, the model enables analysis usually not possible with in vivo samples, such as the quantification of pro-inflammatory cytokines released inside the tissue microenvironment. Our 3D-liver model has the potential to bridge the gap between animal models and the reality of the human host for the study of amoebic infection and other infectious diseases of the liver.


Zdroje

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Štítky
Hygiena a epidemiológia Infekčné lekárstvo Laboratórium

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PLOS Pathogens


2014 Číslo 9
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