The Youth-Physical Activity Towards Health (Y-PATH) intervention: Results of a 24 month cluster randomised controlled trial
Authors:
Sarahjane Belton aff001; Andrew McCarren aff002; Bronagh McGrane aff003; Danielle Powell aff004; Johann Issartel aff001
Authors place of work:
School of Health and Human Performance, Dublin City University, Dublin, Ireland
aff001; School of Computing, Dublin City University, Dublin, Ireland
aff002; School of Arts Education & Movement, Dublin City University, Dublin, Ireland
aff003; Carnegie School Of Sport, Leeds beckett University, Leeds, United Kingdom
aff004
Published in the journal:
PLoS ONE 14(9)
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0221684
Summary
Low levels of physical activity in youth are an issue internationally, with the age related decline in levels over the adolescent period of particular concern. This study evaluated a multi-component school-based intervention (Y-PATH: Youth-Physical Activity Towards Health), focused on halting the age-related decline in physical activity of youth in early adolescence. A cluster randomized controlled trial in 20 post primary schools (10 control, 10 intervention) was conducted. Data were collected from all 20 schools at baseline (2013), and 12 months (2014), and from 10 of these schools (5 intervention) at 24 months (2015). The setting was mixed gender post primary schools residing in the greater area of Dublin, Ireland. Principals from each school were asked to nominate one first year class group attending their school in September 2013 to participate in the study (N = 564). Intervention schools implemented the Y-PATH whole school intervention, comprising teacher component, parent component, and PE component; while control schools continued with usual care. The main outcome measure was accelerometer derived average minutes of daily moderate to vigorous physical activity (MVPA). Data were analysed from October 2015 –November 2017. At baseline 490 participants were assessed (mean age 12.78y ± .42). Results of the multilevel regression analysis confirmed that there was a significant time intervention effect, and this was predominantly contributed by the difference between control and intervention groups within females. Findings support the case for national dissemination of the Y-PATH intervention so that the knowledge learned can be translated to routine practice in schools.
Keywords:
Biology and life sciences – Engineering and technology – Neuroscience – Cognitive science – Cognitive psychology – Learning – Human learning – Learning and memory – Psychology – Social sciences – Sociology – People and places – Population groupings – Professions – teachers – Medicine and health sciences – Public and occupational health – Physical activity – Education – Schools – Age groups – children – Families – Electronics – Sports science – Sports and exercise medicine – exercise – Physical fitness – Ethnicities – European people – Adolescents – Accelerometers – Irish people
Introduction
Despite the known importance and associated benefits of regular physical activity (PA) for health, levels of PA decline dramatically during adolescence [1]. The most widely endorsed PA guideline stipulates that youth should accumulate at least 60 minutes of moderate-to-vigorous PA (≥ 60 min. MVPA) daily [2]. The Health Behaviour in School Aged Children study showed that in Ireland 31% of females, and 43% of males reported accumulating ≥ 60 min of MVPA daily at age 11 [3]. These low figures, which are relatively consistent with the findings of Woods et al.,[4] in a nationally representative Irish sample, declined to 20% of females and 36% of males by age 13 [3]. This decline reflects the critical period of transition of students from primary to post primary education, and is a key period for intervention.
The Centers for Disease Control and Prevention, in a review of evidence for PA promotion strategies [5] concluded that strategies with the potential for greatest reach, effectiveness and sustainability, such as enhanced PE in schools, should be given the highest priority for implementation. Consistent with recent findings [5,6], school-based physical education (PE) was highly recommended as an intervention strategy [7]. A recent review [8] highlighted that comprehensive school based interventions with components targeting both physical activity and health education appear most effective. Van Sluijs et al. [9] in a similar review found that there was strong evidence showing that school-based interventions with a family or community component can increase PA in adolescents (defined as ≥ 10 years). This is supported by the findings of Kriemler et al. [10], who identify school-based application of multi-component intervention strategies as the most promising and consistent strategy for PA intervention with youth, and those of Krahnstoever Davison et al. [11] who demonstrated the importance of peer and family support for PA participation in youth.
The World Health Organisation [12] emphasised the need for research to generate sound knowledge on models of successful intervention in PA. The Waters et al. [13] review indicates that testing short-term, behaviourally focused school-based interventions for 6–12 year old children is no longer warranted, but that the gap in research now relates to effective interventions for adolescents. Van Sluijs et al. [9], in their systematic review of youth PA interventions identified short follow up (less than six months) as a limitation across the studies reviewed. The Salmon et al. [14] review also highlighted short-term follow-up as an issue, and recommend that future studies must include a follow-up of at least 1–2 years. Similarly Dobbins et al. [15] in a Cochrane systematic review of school-based PA programmes, highlighted the need for follow up of PA interventions so that long-term impact can be determined. This lack of long-term follow up along with poorly powered designs as highlighted by Waters at al. [13], may well explain findings of one recent systematic review [16], where a low overall effect for interventions on increasing minutes of MVPA amongst children and adolescents was cited. Waters et al. [13] recommend larger, sustainable and longer-term studies, guided by theories such as the socio-ecological model, powered to detect the small changes that are likely to be found.
Following the recommendations and targeted strategies alluded to above, the Y-PATH (Youth-Physical Activity Towards Health) programme was developed as a research-informed and evidence-based multi component school-based intervention [17]. Y-PATH aims to improve PA levels of adolescents through (i) education about the importance of PA for health and the dangers of sedentary behaviour, (ii) increasing proficiency in basic movement skills essential for participation in PA known as fundamental movement skills (FMS), and (iii) improving levels of self-efficacy motivation, regulation, and empowerment. In addition to targeting the adolescent at the individual level, Y-PATH also extends to the whole school by targeting the teachers and parents within the intervention. The Y-PATH programme is the first evidence-based intervention of its kind in Ireland, and was developed following cross-sectional exploratory research [18], using a social-ecological framework, and based on the Youth Physical Activity Promotion Model [19], with subsequent development of the teacher education element of the intervention based on Self-Determination Theory [20,21]. Central to the development of the Y-PATH intervention at all stages was the need for the intervention to be cost-effective, time-efficient for teachers, and scalable to a national level.
Previous research on the Y-PATH intervention has included a one-year two school exploratory trial [21] and a one-year 20-school cluster randomized control trial [22]. In both of these trials, an intervention effect was found for FMS proficiency (measured using the Test of Gross Motor Development—Second Edition) [23] (Ulrich, 2000), while an intervention effect for PA (measured by self-report and objective accelerometry) was found in the exploratory trial only [21]. The purpose of the present study is to build on this previous work, and address the research gaps alluded to above, to investigate the effect of participation in the Y-PATH intervention over a two-year period on objectively measured MVPA levels of young people.
Methods
Study design, setting and participants
The Y-PATH cluster randomized controlled trial targeted first year post primary students (12–13 years old) attending post primary education within a particular Irish geographical region. Inclusion criteria for post primary schools in this study were that a) schools have a qualified PE teacher on staff, b) first year students attending the school were timetabled for a minimum of 70 minutes of PE weekly, c) schools were mixed gender and situated in the greater area of a large Irish city. All mixed-gender schools in the particular Irish geographical region (n = 104) were invited to express interest in participation in the study if they met the above inclusion criteria. This trial was registered retrospectively with the ISRCTN (International Standard Registered Clinical/soCial sTudy Number), and the trial record is available at http://www.isrctn.com/ISRCTN20495704.
Sample size estimation was carried out by considering the 12% of Irish adolescents estimated to meet the 60-minute daily MVPA guideline [4] nationally in Ireland. A total of 18 schools (9 per arm, with an average of 27 participants per school) were estimated to provide at least 80% power at a 5% level of significance (2-sided) to detect a 20% difference (with an intraclass correlation of 0.1) in the proportion of children meeting the 60-minute MVPA guidelines at 12 months. To allow for attrition, a further 2 schools per arm (with 27 students per school) were required, increasing the targeted sample to 20 schools. Principals of 26 schools returned expressions of interest, screening of these schools highlighted that 22 schools met the inclusion criteria, all 22 schools were recruited to participate in the study. One first year class group from each school was randomly selected by the school principal to participate. Two schools subsequently withdrew from the study prior to commencement due to changes in staffing (PE teacher and principal), reducing numbers to 20 overall.
Randomization was carried out at the school level rather than at the student level, to minimize the possibility of students or teachers in the control groups being influenced by intervention group participants. The 20 recruited schools were pair-matched prior to baseline testing based on the following criteria: socioeconomic status (disadvantaged, non-disadvantaged, and fee paying), school size (small 0–299 students, medium 300–599 students, large >599 students), and facilities (school hall, size of hall, basketball courts etc.). One school from each pair was then randomly allocated by the study principal investigator to the control group (and the other to the intervention group) using a manual number generator in blocks of 1 : 1, prior to the commencement of baseline testing.
Outcome assessments were conducted with students in all 20 schools at baseline (T1, September-October 2013), at 12 months follow up (T2, September-October 2014). All schools were invited to remain involved in the study for a further 12 months. Ten of these schools (5 intervention and 5 controls) consented to remain involved in the study, with the remaining ten unable to continue beyond the original 12-month commitment for a variety of reasons (including change in principal or PE teacher, and commitments already made to other PA initiatives for the following 12 months). As such, outcome assessment was conducted with this sub-sample of 10-schools at 24 months follow up (T3, September-October 2015). Principal consent, opt-in written parental consent, and participant assent were required prior to data collection. All participants were free to withdraw from the research at any stage. Full approval for this study was granted by the Dublin City University Research Ethics Committee (DCUREC/2010/081). CONSORT guidelines were followed to ensure no bias was observed in this study. Fig 1 documents the flow of participants through the study. The lower numbers at T2 compared to T1 are explained by i) children’s absence from school on the day of testing, ii) children choosing to withdraw from the study, and iii) injury/illness that prevented them from completing the protocol. The fewer schools involved at T3 explains in large part the lower number at this time point, along with reasons i) to iii) outlined above.
Flow of participants through the study.
Intervention
Guiding principles
The theoretical framework driving the Y-PATH intervention is shown in Fig 2. Full detail on the development of the Y-PATH intervention, and of the various components involved in the intervention, are given in Belton et al. [18]. The guiding principles of the intervention are given below:
The first experience of PE for the students at second level school will be Health-Related Activity, with a focus on PA participation (move from PE being associated with a specific activity or sport, to being associated with learning to be active)
PE lessons will focus on maintaining MVPA levels, and improving students’ attitude towards PA, self-efficacy and FMS levels.
The climate in PE lessons will be motivational; all students learn that they can be active, experience a range of choice, and learn to challenge themselves and experience success within their own parameters (focus on attitude and self-efficacy).
Parents/guardians and non-specialist PE teachers targeted as role models that can have a significant influence on students’ attitudes towards PA participation (move from traditional notion of PE teacher being the person in the school with sole responsibility for health and PA).
Theoretical framework of the Y-PATH intervention.
Components of the Y-PATH intervention
The Y-PATH intervention is a whole-school multi-component intervention programme, aimed at reducing the age-related decline of MVPA in adolescents. The different components target students, teachers and parents, with a PE component, a whole-school teacher component and a parent component.
PE Component: Y-PATH PE has a strong focus on physical literacy development (developing student motivation, self-confidence, FMS mastery, physical fitness, and Health-Related Activity knowledge) within the PE class, with the school’s qualified PE teacher trained to deliver Y-PATH PE over the full academic year. Crucially, Y-PATH PE does not add to the existing teaching requirements of the PE teacher, rather offers a renewed structure and emphasis through which to deliver the existing state curricula for PE at Junior Cycle. Y-PATH PE fully upholds the existing Department of Education and Skills’ Junior Cycle Physical Education Curriculum [24], but changes the focus, direction and philosophy of delivery.
PE teachers in the intervention condition received four hours of Y-PATH Continuing Professional Development on the implementation of the Y-PATH-PE element, prior to the commencement of the academic school year. As part of this element of the intervention the PE teachers receive a set of six targeted lesson plans to be delivered by the PE teacher to their class groups for the first six weeks of the academic year in first year, and a second set to be delivered for the first six weeks of the second academic year. These lessons focus heavily on motivational climate, integrating health related activity core knowledge through fun and engaging practical lessons, with an emphasis on FMS proficiency throughout. For the remainder of the school year, the teachers are given a set of resource cards as teaching prompts to enable them to integrate a Health-Related Activity and FMS focus within the other core PE content areas (Gymnastics, Dance, Aquatics, Games, Outdoor and Adventure Activities, and Athletics) while teaching for a motivational climate. Y-PATH PE is supported by a student ‘PA journal’ that teachers are asked to integrate as part of regular PE class, so that students learn to track their PA behaviours and identify ways they could improve their PA levels. In addition, a local ‘PA directory’ that contains information and contact details for a range of youth sport and PA clubs in the local community is provided, to assist PE teachers in linking students with PA opportunities beyond the school.
Whole-School Component: The whole-school component included two ‘PA Promotion’ workshops for teachers delivered by a Y-PATH-trained facilitator, as well as the development and implementation of a school ‘charter’ for physical activity with specific targets agreed by the school community. All teachers within the school are encouraged to be ‘active role models’ for students.
Parent Component: This included an information evening delivered by a Y-PATH-trained facilitator, and a parents’ PA information leaflet distributed periodically through the school newsletter. Both the information evening and the information leaflets highlight key strategies for promoting PA beyond the school environment which are discussed with parents and emphasized periodically.
Schools allocated to the intervention condition were asked to implement the whole-school Y-PATH intervention over the full academic year, the 5 intervention schools that remained in the study for the second academic year were asked to implement the intervention for this second year also. Control schools were asked to continue with usual care, without any researcher input over the academic year. Usual care in this context consisted of regular delivery of the Irish Junior Cycle PE curriculum, and their broader school curricula.
Data collection
Measures taken in this study were accelerometer measured MVPA, and body mass index (BMI). The measurements are detailed below, and were taken by trained research assistants who visited the schools at each of the three time points (T1, T2, and T3). Research assistants were not blinded to control or intervention condition assignment as it was not possible given the nature of the intervention (for example Y-PATH teaching posters are displayed on school walls as part of the intervention).
Accelerometer data was used to derive the primary outcome measure, mean student duration (minutes) of MVPA per day. Participants were asked to wear an Actigraph accelerometer (models GT1M, GT3X or GT3X+; Actigraph LLC, Pensacola, FL) on an adjustable elasticated belt above the iliac crest of the right hip. Participants were asked to wear the accelerometer during all waking hours, with the exception of water-based activities such as showering and swimming, and contact sports deemed unsafe for accelerometer wear (e.g. rugby) for a total of nine consecutive days. Accelerometers were set to record using a 10-second epoch [25]. A number of strategies were employed to maximize wear-time compliance [26]: students were met in the morning of each school day to ascertain compliance with the wear instructions; an optional twice daily SMS reminder text was sent to students before school, and in the afternoon immediately after school; a specific teacher in each school checked whether or not participants were wearing their monitors during each school day; students were advised to place reminders to wear monitors in noticeable areas in their homes; and students who were compliant with the wear-time inclusion criteria, entered a class draw for a €20 sports voucher (per class).
In line with previous studies [25,27], the minimum number of valid days required for inclusion in accelerometer data analysis was two days [28], a day was deemed valid (and therefore included in analysis) if there was a minimum of 8 hours recorded wear-time per day [29], and monitor non-wear was defined as ≥20 consecutive minutes of zero counts. Any counts below zero and above 15,000 were excluded, due to biological implausibility [30]. The mean daily minutes spent in moderate-to-vigorous physical activity (MVPA) were estimated using the validated cut points, derived by Evenson et al. (2008) for adolescents in this age group: MVPA ≥ 2296 counts/min.
Standing height was measured using a portable stadiometer (Leicester Height Measure) in centimetres (cm) to the nearest two decimal places. Weight was measured using a portable calibrated scales (SECA) in kilograms (kg) to the nearest 0.5kg. BMI was calculated using the formula weight(kg)/height2(m2). Participants completed the measurements in light clothing without shoes.
Statistical analysis
Multilevel linear regression analysis was used to examine the effect of the Y-PATH intervention on average minutes of MVPA (Total MVPA). A three level multilevel structure was proposed with random intercepts, where time (Level one), pupils (Level two) and schools (Level three) served as the grouping variables, where time was treated as a fixed effect in the model but was also incorporated as a random slope effect (repeated measure) in the residual component. The structure is outlined in Eqs 1–5. All fixed effect interactions were examined. The repeated measures component was analyzed for unstructured, unstructured heterogeneous Eq (5), autoregressive, autoregressive heterogeneous and compound symmetry variance structures.
The model outlined in Eq 1 also had higher order time covariates incorporated to examine the possibility of a non-linear time effect on MVPA. Heterogeneity of treatment effects was examined by incorporating an additional interaction between treatment and the baseline BMI and baseline MVPA covariates. Regression coefficients for the group variables (where ‘0’ indicated Control schools, and ‘1’ indicated Intervention schools) reflected average differences in the outcome variable over time adjusted for baseline outcome values, timing of follow-up measures, and a priori covariates known to moderate MVPA (gender and BMI) over 3 time periods. To determine the time points at which any intervention effects occurred at (T1 (0), T2 (1), or T3 (2)), post-hoc stratified analyses comparing the estimated marginal means of the interaction variables were performed for the Intervention and the Control groups, and comparisons were made with t-tests using Satterwhaite degrees of freedom. Random Intercepts were assessed for significance using the Wald statistic with statistical significance set at p<0.05. The covariance structure of the mixed model outlined in Eq 1 was evaluated by assessing the Akaike (AIC) information criterion and Bayesian information criterion (BIC). Analyses were performed using SPSS software version 24 (IBM Corporation).
Results
A total of 490 participants from 20 schools were involved in this research at baseline (T1), this dropped to 420 participants from 20 schools at 12-month follow-up (T2), and to 249 participants from 10 schools at 24-month follow-up (T3). Baseline characteristics of the participant sample by gender and intervention condition are given in Table 1. An independent samples t-test revealed no significant differences in age or BMI between participants that met or did not meet the accelerometer inclusion criteria. Minutes of MVPA by gender and intervention condition across the three time points are given in Table 2, and shown graphically in Fig 3.
Baseline characteristics (mean ± standard deviation) by gender and intervention condition.
Sample size and MVPA (minutes/day) at each time point by gender and intervention condition.
MVPA (minutes/day) across time and gender for control and intervention groups.
An Autoregressive (AR) covariance structure was found to have the lowest AIC and BIC. The random intercept for School was found to be marginally insignificant (21.439, p = 0.051, Wald Z = 1.953), and the AR diagonal (233.255, p<0.001, Wald Z = 19.467) and the AR ρ (.125, p = 0.01, Wald Z = 2.574) of the error term were found to be significant. In this model the intercept term at a student level was excluded, as it was described fully by AR covariance structure. The higher order powers of time, the interaction between baseline BMI and treatment were excluded from the final model as they were highly insignificant and they had no direct impact on lower order model terms. Additionally, including baseline BMI would have reduced the data volume by over 10% due to the missing values.
The final parameter estimates for the fixed effects of the final model choice are shown in Table 3, and similarly the Type III test effects for the final interaction effects are shown in Table 4.
Parameter estimates of main fixed effects.
Type III analysis of interaction effects.
Post hoc analysis on the group comparisons of the intervention interaction with time are outlined in Table 5.
Post hoc contrast test analysis for significant interaction effects.
Discussion
The purpose of this study was to investigate the effect of participation in the Y-PATH intervention over a two-year period on objectively measured MVPA levels of young people. Data collected and analysed in this study confirm the well-known and frequently observed gender disparity in physical activity levels in youth [31,4,32] and the frequently reported age related decline in physical activity [31,4,32]; with males average daily MVPA dropping by 5.79 minutes over the two year period, and females average daily MVPA dropping by 10.33 minutes over the same period (the decline further exaggerating the gender disparity). Study findings provide some support for the efficacy of the Y-PATH intervention. After 24 months, the intervention was effective in maintaining MVPA levels specifically in females in the intervention condition, while control participants levels significantly declined. The findings suggest that implementing the Y-PATH intervention in post primary schools may have the potential to help address the age-related decline in female MVPA levels.
This study evaluating the Y-PATH intervention builds upon and supports the positive findings demonstrated in previous research [27,22] for the efficacy of the Y-PATH programme. Fig 3 graphically presents the trends in average daily minutes of MVPA across gender and time for control and intervention conditions. From this graph the downward trend in MVPA over time is apparent for all groups, with the biggest decline evident for males in the control condition (where a higher level of MVPA at baseline was observed), and females in the control condition. Results of the mixed model analysis confirmed no main effect for BMI, but showed a significant time*intervention effect which was predominantly contributed by the impact of the intervention on females. The significant interaction effects found within the data are presented in Table 4. The post hoc analysis in Table 5 demonstrates how the interaction between time and intervention occurs. One can see that there was no significant difference between control and intervention at baseline, but a significant difference is evident between the control and intervention at 24-months; with control participants demonstrating a significant decline in minutes of MVPA over the course of the intervention period. Findings are consistent with the results of the Physical Activity 4 Everyone cluster randomised trial in Australia [33], a trial similar to that reported in the current study. Sutherland et al. [33] evaluated the 24-month intervention effect of the multicomponent school based Physical Activity 4 Everyone intervention in disadvantaged schools (children aged 12 years at baseline). Sutherland et al. [33] reported that the intervention was effective in increasing adolescents minutes of MVPA, and concluded that implementing the intervention may have the potential to slow the age related decline in physical activity.
This finding is also consistent with the results of the HEalth in Adolescents (HEIA) study in Norway [34], which evaluated the intervention effect of the HEIA 20-month multicomponent school based intervention (children aged 11 years at baseline), and similarly found a stronger effect in females. The reason hypothesized by Grydeland et al. [34] for this gender effect, was that the intervention while not developed specifically for females, it was developed bearing low levels of female physical activity in mind. The same is true for Y-PATH PE. Moreover, the pedagogical focus within Y-PATH PE was to move PE class towards motivational climate, with an emphasis on student mastery rather than competition. Considering the consistent message from research through the years highlighting competition as a barrier to PE participation and enjoyment for females particularly [35–37], it may well be this pedagogical focus which explains the stronger finding for females in this study.
The positive finding in terms of females who experienced the Y-PATH intervention in the current study are particularly encouraging when we consider findings of a recent systematic review and meta-analysis of the effectiveness of school based physical activity interventions in adolescent girls [38]. These authors [38] concluded, concluded, based on the small intervention effects observed across studies reviewed, that changing PA behaviour of adolescent females through school based interventions is challenging. The authors did go on to state however that multi-component interventions, and interventions based on theory may provide the strongest chance of positively impacting PA behaviour of this at risk cohort [38]; the fact that Y-PATH meets both of these criteria may well explain the positive result found in this study.
Similarly in another systematic review [39] it was concluded that PA was a sustainable outcome from interventions in children and adolescents, with interventions such as Y-PATH which are longer than 1 year in duration and based on a sound theoretical model more likely to achieve sustained impact. This is consistent with the recent work of Meyer et al. [40] who advocated the need for longer sustained intervention programmes for sustained impact on health outcomes. In a recent systematic review of school based interventions aimed at increasing physical activity (and fitness) levels of youth [10], the authors conclude that school based application of multi-component interventions was the most consistent and promising strategy of those reviewed. The results presented in this paper on the outcomes of the Y-PATH intervention add further support to the conclusions’ of Kriemler et al., [10], and further point to the important role of school based multi-component interventions as a strategy for increasing physical activity levels of youth.
The importance of targeted strategic intervention development and evaluation is also highlighted in the positive findings of this study. Y-PATH was developed based on careful consideration of the context and situation of the specific target cohort of adolescent Irish youth [18] to ensure it could meet their needs, and would work within the broad school and PE environment, specific to the Irish context. The Medical Research Council’s [41] advice on the development and evaluation of complex interventions was respected, with initial pilot, feasibility and evaluation research [18,27,22], followed by the two-year definitive trial reported in this paper. The partnerships developed over the course of this work, as documented in Belton et al. [17], have included local and national stakeholders to ensure that the next step of the Medical Research Council guidelines could be followed; the implementation phase where intervention findings can be translated into routine practice. The need for an intervention such as Y-PATH to address physical activity problems of Irish youth has been very well explicated in recent years at a national level. In 2016 Ireland published the first time a National Physical Activity Plan [42], with interagency agreement between Government departments. Action area two defined in the National Physical Activity Plan focuses on children and young people, with the specific target being that ‘Children and young people learn the necessary skills for confident engagement with physical activity and will have opportunities to adopt an active way of life’ (pp 17; [42]. The government Departments which collaborated in the development of this plan highlighted that being active was central to health of children and young people, with key emotional, social and cognitive benefits. They further identified the importance of the school setting, a location where ‘the knowledge, skills and behaviours which are likely to enhance lifelong engagement in physical activity and good health’ should be developed (pp 17; [42]). Of key note in this document was the recognition of the role the school system should play, and in particular quality PE, in enabling children to lead physically active lives through the mastery of fundamental skills, and the acquisition of relevant knowledge and positive attitudes.
These elements of quality PE recognised in the National Physical Activity Plan [42], are consistent with those highlighted in the concept of ‘physical literacy’, a term which is growing in use in recent years. Physically literate people are those that move well, move often, and move confidently in a variety of situations to the benefit of their health [43–45]. Through the research carried out in the development of Y-PATH, the importance of positive attitudes, self-efficacy, motivation, and FMS ability to enable youth to be active was highlighted [18]; and the programme was built to satisfy these physical literacy needs. Y-PATH is a research-informed and evidence-based intervention programme [18,27,22], which meets many of the goals and recommendations of Irelands National Physical Activity Plan [42] as highlighted above. Perhaps more importantly when national implementation is considered as a target however, the Y-PATH intervention was also developed with and for schools, and specifically to uphold and support the core tenets of the national PE curriculum for youth this age [46,24,18]. The findings of this study support that the Y-PATH intervention which was developed to meet the specific needs of Irish youth, can play a role in helping to alleviate the known age related decline in PA that happens over this critical adolescent period. More recent research on the Y-PATH programme has seen the development of a targeted and sustainable national implementation plan, with multi agency support to ensure that the knowledge learned through this research can be translated into routine practice in Irish schools [17]. In September 2018, the Irish Heart Foundation committed to national dissemination of Y-PATH, with a target of reaching every school in the country by 2021. Dissemination is being led by the Irish Heart Foundation, in partnership with Dublin City University, and supported by University College Cork, Sport Ireland, and the Professional Development Service for Teachers (state body with responsibility for provision of professional development to teachers in Ireland)[17]. Further research to confirm the findings of this study on a larger scale, and track fidelity as the programme is scaled up, is warranted as the programme is rolled out to more schools nationally.
Strengths and limitations
Following recommendations of other authors this evaluation involved interim measurements over the rollout period [15], and with retention follow-up of at least one year [9,47]. As recommended in these review papers [15,13,9], an objective measure of PA (accelerometry) was employed as the primary outcome measure, and as advocated by Kriemler et al., [10], measurement wasn’t restricted to the school day but included total PA. The choice of two days as our wear criterion may be considered a limiting factor, and in line with the discussions presented in Rich et al. [28] was chosen to strike a balance between reliability of estimates and sample size. The poor compliance with accelerometer wear criteria must be acknowledged as a limitation however, with 86% meeting the 2 day criterion at T1, 60% at T2, and 82% (of the reduced sample of 10 schools) at T3. This issue is common across intervention studies such as that presented in this paper [48]. The smaller sample size at T3 due to the limited number of schools participating in data collection at 24 months means that changes in PA levels at follow-up testing should be interpreted with caution, as the mean PA levels reflect only a sub-sample of participants. The fact that this study included only mixed gender schools means that results are delimited to this cohort, and cannot be generalised to participants attending single gender schools. Given the Y-PATH programme itself targets physical activity self-efficacy and motivation (among other factors), future research should investigate these as potential mediators through which the Y-PATH intervention exerted it’s effect on MVPA, something which was not considered in this study. Finally, although the Y-PATH intervention may be considered relatively low cost, with no personnel cost involved in rollout aside from that incurred in CPD delivery, in order to truly determine the efficacy a cost-effectiveness analysis may be warranted.
Conclusions
Findings of this study support the efficacy of the Y-PATH intervention in helping to alleviate the age-related decline in MVPA over a two-year period in adolescent youth. Results provide further support for the efficacy of such school-based multi-component interventions. Translation of the research findings to routine practice in Irish schools is a critical next step for the intervention programme if it is to have a lasting effect on public health. The commitment of key stakeholders nationally to a sustainable national dissemination plan in this regard is a considerable step forward. Future research should investigate whether the effect of the Y-PATH intervention is specific to school day or weekend MVPA, whether the effect would remain consistent across single gender schools and with a larger sample as it is scaled up, and if the intervention would continue to have an effect beyond the two-year period.
Zdroje
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- Clinical correlates of workplace injury occurrence and recurrence in adults
- Medicine and the media: Medical experts’ problems and solutions while working with journalists
- Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli strains isolated from community acquired infections
- Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique
- Forest resilience under global environmental change: Do we have the information we need? A systematic review
- Improving the production of podophyllotoxin in hairy roots of Hyptis suaveolens induced from regenerated plantlets
- Controls on planktonic foraminifera apparent calcification depths for the northern equatorial Indian Ocean
- Notch and Delta are required for survival of the germline stem cell lineage in testes of Drosophila melanogaster
- Children with HIV: A scoping review of auditory processing skills
- Correction: Two distinct actin waves correlated with turns-and-runs of crawling microglia
- Expression of Concern: Neurotoxicity Induced by Bupivacaine via T-Type Calcium Channels in SH-SY5Y Cells
- Sex-dependent and -independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissima
- Vehicle modeling for the analysis of the response of detectors based on inductive loops
- New Mycobacteroides abscessus subsp. massiliense strains with recombinant hsp65 gene laterally transferred from Mycobacteroides abscessus subsp. abscessus: Potential for misidentification of M. abscessus strains with the hsp65-based method
- A prognostic Bayesian network that makes personalized predictions of poor prognostic outcome post resection of pancreatic ductal adenocarcinoma
- Impact of care provider network characteristics on patient outcomes: Usage of social network analysis and a multi-scale community detection
- Valid group comparisons can be made with the Patient Health Questionnaire (PHQ-9): A measurement invariance study across groups by demographic characteristics
- Comparison of risk models for mortality and cardiovascular events between machine learning and conventional logistic regression analysis
- Upgraded molecular models of the human KCNQ1 potassium channel
- Mental health and quality of life outcomes in family members of patients with chronic critical illness admitted to the intensive care units of two Brazilian hospitals serving the extremes of the socioeconomic spectrum
- Correction: Secretome profiling of PC3/nKR cells, a novel highly migrating prostate cancer subline derived from PC3 cells
- The extraordinary osteology and functional morphology of the limbs in Palorchestidae, a family of strange extinct marsupial giants
- Primary hyperhidrosis prevalence and characteristics among medical students in Rio de Janeiro
- Implementation of home blood pressure monitoring among French GPs: A long and winding road
- In vitro and molecular chemosensitivity in human cholangiocarcinoma tissues
- Saiga horn user characteristics, motivations, and purchasing behaviour in Singapore
- The health and cost burden of antibiotic resistant and susceptible Escherichia coli bacteraemia in the English hospital setting: A national retrospective cohort study
- Retraction: Comprehensive Comparison of Three Different Immunosuppressive Regimens for Liver Transplant Patients with Hepatocellular Carcinoma: Steroid-Free Immunosuppression, Induction Immunosuppression and Standard Immunosuppression
- Can acute suicidality be predicted by Instagram data? Results from qualitative and quantitative language analyses
- Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi
- Antibacterial activity of silver nanoparticles of different particle size against Vibrio Natriegens
- Genetic variations associated with response to dutasteride in the treatment of male subjects with androgenetic alopecia
- To eat or not to eat: Reward delay impulsivity in children with loss of control eating, attention deficit / hyperactivity disorder, a double diagnosis, and healthy children
- Factors associated with newborn care knowledge and practices in the upper Himalayas
- Correction: A more physiological approach to lipid metabolism alterations in cancer: CRC-like organoids assessment
- Correlates of concurrent partnerships and patterns of condom use among men who have sex with men and transgender women in Peru
- Ocular and systemic risk factors associated with recurrent disc hemorrhage in primary open-angle glaucoma
- Development of a denoising convolutional neural network-based algorithm for metal artifact reduction in digital tomosynthesis for arthroplasty: A phantom study
- Low-level sensory processes play a more crucial role than high-level cognitive ones in the size-weight illusion
- The prevalence of asthma and allergic rhinitis in Nigeria: A nationwide survey among children, adolescents and adults
- Retraction: Human Cystathionine-β-Synthase Phosphorylation on Serine227 Modulates Hydrogen Sulfide Production in Human Urothelium
- Which clinical and biochemical predictors should be used to screen for diabetes in patients with serious mental illness receiving antipsychotic medication? A large observational study
- Characteristics of diabetic macular edema patients refractory to anti-VEGF treatments and a dexamethasone implant
- Correction: Effects of dietary n-6: n-3 polyunsaturated fatty acid ratios on meat quality, carcass characteristics, tissue fatty acid profiles, and expression of lipogenic genes in growing goats
- Speckle tracking derived reference values of myocardial deformation and impact of cardiovascular risk factors – Results from the population-based STAAB cohort study
- Impact of pterygium on the ocular surface and meibomian glands
- Lack of association between hypothyroxinemia of prematurity and transient thyroid abnormalities with adverse long term neurodevelopmental outcome in very low birth weight infants
- Transcriptome divergence during leaf development in two contrasting switchgrass (Panicum virgatum L.) cultivars
- Study on Dalfampridine in the treatment of Multiple Sclerosis Mobility Disability: A meta-analysis
- Circulating progenitor cells and the expression of Cxcl12, Cxcr4 and angiopoietin-like 4 during wound healing in the murine ear
- EUSKOR: End-to-end coreference resolution system for Basque
- Inactivating pathogenic bacteria in greywater by biosynthesized Cu/Zn nanoparticles from secondary metabolite of Aspergillus iizukae; optimization, mechanism and techno economic analysis
- Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions
- Structural equation modeling for hypertension and type 2 diabetes based on multiple SNPs and multiple phenotypes
- Correction: Association between ultraviolet radiation exposure dose and cataract in Han people living in China and Taiwan: A cross-sectional study
- Short-term exercise training improves cardiac function associated to a better antioxidant response and lower type 3 iodothyronine deiodinase activity after myocardial infarction
- Unexpected low genetic variation in the South American hystricognath rodent Lagostomus maximus (Rodentia: Chinchillidae)
- PRC2 activates interferon-stimulated genes indirectly by repressing miRNAs in glioblastoma
- Surgical resection is sufficient for incidentally discovered solitary pulmonary nodule caused by nontuberculous mycobacteria in asymptomatic patients
- “I do all I can but I still fail them”: Health system barriers to providing Option B+ to pregnant and lactating women in Malawi
- Impact of major illnesses and geographic regions on do-not-resuscitate rate and its potential cost savings in Taiwan
- Implementation of intermittent theta burst stimulation compared to conventional repetitive transcranial magnetic stimulation in patients with treatment resistant depression: A cost analysis
- Genome-wide analysis of DNA methylation profile identifies differentially methylated loci associated with human intervertebral disc degeneration
- Correction: Artificial neural networks reveal individual differences in metacognitive monitoring of memory
- Combining fish and environmental PCR for diagnostics of diseased laboratory zebrafish in recirculating systems
- Reading skill modulates the effect of parafoveal distractors on foveal lexical decision in deaf students
- Association between the posterior part of the circle of Willis and the vertebral artery hypoplasia
- Exposure to marital conflict: Gender differences in internalizing and externalizing problems among children
- Ethanol locks for the prevention of catheter-related infection in patients with central venous catheter: A systematic review and meta-analysis of randomized controlled trials
- The effect of age and perturbation time on online control during rapid pointing
- Oilseed rape (Brassica napus) resistance to growth of Leptosphaeria maculans in leaves of young plants contributes to quantitative resistance in stems of adult plants
- Lymphocyte proliferation induced by high-affinity peptides for HLA-B*51:01 in Behçet’s uveitis
- Sensitization of avian pathogenic Escherichia coli to amoxicillin in vitro and in vivo in the presence of surfactin
- Genomic characterization of the complete terpene synthase gene family from Cannabis sativa
- Inhibition of gap junctional intercellular communication by an anti-migraine agent, flunarizine
- Levels of serum eosinophil cationic protein are associated with hookworm infection and intensity in endemic communities in Ghana
- Plant growth promoting rhizobacterium Stenotrophomonas maltophilia BJ01 augments endurance against N2 starvation by modulating physiology and biochemical activities of Arachis hypogea
- Hierarchical integrated and segregated processing in the functional brain default mode network within attention-deficit/hyperactivity disorder
- Rising co-payments coincide with unwanted effects on continuity of healthcare for patients with schizophrenia in the Netherlands
- Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
- False-negative errors in next-generation sequencing contribute substantially to inconsistency of mutation databases
- Identification of potassium phosphite responsive miRNAs and their targets in potato
- Quantification of bell-shaped size selectivity in shrimp trawl fisheries using square mesh panels and a sorting cone after a Nordmøre grid
- Deactivation of somatosensory and visual cortices during vestibular stimulation is associated with older age and poorer balance
- Combined immunization with attenuated live influenza vaccine and chimeric pneumococcal recombinant protein improves the outcome of virus-bacterial infection in mice
- Flooding performance evaluation of alkyl aryl sulfonate in various alkaline environments
- An artificial intelligent diagnostic system on mobile Android terminals for cholelithiasis by lightweight convolutional neural network
- Enhanced detection of prion infectivity from blood by preanalytical enrichment with peptoid-conjugated beads
- COI metabarcoding primer choice affects richness and recovery of indicator taxa in freshwater systems
- Retraction: APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway
- The intake pattern and feed preference of layer hens selected for high or low feed conversion ratio
- Individualized pattern recognition for detecting mind wandering from EEG during live lectures
- Ontogenetic expression of thyroid hormone signaling genes: An in vitro and in vivo species comparison
- Post-activation potentiation effect of eccentric overload and traditional weightlifting exercise on jumping and sprinting performance in male athletes
- Effectiveness of different central venous catheter fixation suture techniques: An in vitro crossover study
- Quantitative detection of ALK fusion breakpoints in plasma cell-free DNA from patients with non-small cell lung cancer using PCR-based target sequencing with a tiling primer set and two-step mapping/alignment
- Nuclei deformation reveals pressure distributions in 3D cell clusters
- Strategies for increasing diagnostic yield of community-onset bacteraemia within the emergency department: A retrospective study
- Rapid evolution of Mexican H7N3 highly pathogenic avian influenza viruses in poultry
- Certified service dogs – A cost-effectiveness analysis appraisal
- Endothelial dysfunction and low-grade inflammation in the transition to renal replacement therapy
- Assessing the impact of a research funder’s recommendation to consider core outcome sets
- Validity of six consumer-level activity monitors for measuring steps in patients with chronic heart failure
- Detection of deceptive motions in rugby from visual motion cues
- The impact of antenatal care on neonatal mortality in sub-Saharan Africa: A systematic review and meta-analysis
- Epidemiological investigation and management of bloody diarrhea among children in India
- Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation
- Bacteria isolated from Bengal cat (Felis catus × Prionailurus bengalensis) anal sac secretions produce volatile compounds potentially associated with animal signaling
- Petri net–based model of the human DNA base excision repair pathway
- Complexation and conformation of lead ion with poly-γ-glutamic acid in soluble state
- The effects of sympathetic activity induced by ice water on blood flow and brachial artery flow-mediated dilatation response in healthy volunteers
- The Youth-Physical Activity Towards Health (Y-PATH) intervention: Results of a 24 month cluster randomised controlled trial
- The COPD multi-dimensional phenotype: A new classification from the STORICO Italian observational study
- Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data
- Maintaining hope after a disabling stroke: A longitudinal qualitative study of patients’ experiences, views, information needs and approaches towards making treatment decisions
- Cultural differences in the use of acoustic cues for musical emotion experience
- Effects of a rifampicin pre-treatment on linezolid pharmacokinetics
- High prevalence of multidrug resistant Enterobacteriaceae among residents of long term care facilities in Amsterdam, the Netherlands
- Infection/inflammation-associated preterm delivery within 14 days of presentation with symptoms of preterm labour: A multivariate predictive model
- Prognostic value of preoperative hydronephrosis in patients with bladder cancer undergoing radical cystectomy: A meta-analysis
- Correction: Early life predictors of midlife allostatic load: A prospective cohort study
- PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
- Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments
- Symbiotic incompatibility between soybean and Bradyrhizobium arises from one amino acid determinant in soybean Rj2 protein
- Does anticoagulation needed for distally located incidental pulmonary thromboembolism in patients with active cancer?
- Control of human testis-specific gene expression
- Speaking up culture of medical students within an academic teaching hospital: Need of faculty working in patient safety
- Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations
- Understanding parental perspectives on outcomes following paediatric encephalitis: A qualitative study
- Results of scoping review do not support mild traumatic brain injury being associated with a high incidence of chronic cognitive impairment: Commentary on McInnes et al. 2017
- Native seed, soil and atmosphere respond to boreal forest topsoil (LFH) storage
- Development of a quantitative polymerase chain reaction assay and environmental DNA sampling methods for Giant Gartersnake (Thamnophis gigas)
- Genetic diversity and population structure of four Chinese rabbit breeds
- Knowledge, attitude and behaviors towards patients with mental illness: Results from a national Lebanese study
- Correction: An impact evaluation of two rounds of mass drug administration on the prevalence of active trachoma: A clustered cross sectional survey
- Comparative prognostic accuracy of sepsis scores for hospital mortality in adults with suspected infection in non-ICU and ICU at an academic public hospital
- Graph convolutional network approach applied to predict hourly bike-sharing demands considering spatial, temporal, and global effects
- Impact of traffic variability on geographic accessibility to 24/7 emergency healthcare for the urban poor: A GIS study in Dhaka, Bangladesh
- Feeling the heat: Elevated temperature affects male display activity of a lekking grassland bird
- Constructing a comprehensive disaster resilience index: The case of Italy
- Intraocular pressure according to different types of tonometry (non-contact and Goldmann applanation) in patients with different degrees of bilateral tearing
- Prevalent vertebral fracture is dominantly associated with spinal microstructural deterioration rather than bone mineral density in patients with type 2 diabetes mellitus
- Silent volumetric multi-contrast 7 Tesla MRI of ocular tumors using Zero Echo Time imaging
- Nonverbal synchrony in virtual reality
- Do speed cameras reduce road traffic collisions?
- A zero-shot learning approach to the development of brain-computer interfaces for image retrieval
- Use of non-HIV medication among people living with HIV and receiving antiretroviral treatment in Côte d’Ivoire, West Africa: A cross-sectional study
- DNA barcoding of southern African crustaceans reveals a mix of invasive species and potential cryptic diversity
- Development of an international external quality assurance program for HIV-1 incidence using the Limiting Antigen Avidity assay
- Correction: The mean cell volume difference (dMCV) reflects serum hypertonicity in diabetic dogs
- Structural characteristics of lipocalin allergens: Crystal structure of the immunogenic dog allergen Can f 6
- Transport oil product consumption and GHG emission reduction potential in China: An electric vehicle-based scenario analysis
- Ontogenetic shift in the energy allocation strategy and physiological condition of larval plaice (Pleuronectes platessa)
- Increased proliferation and altered cell cycle regulation in pancreatic stem cells derived from patients with congenital hyperinsulinism
- Diagnostic accuracy of Xpert MTB/RIF assay and non-molecular methods for the diagnosis of tuberculosis lymphadenitis
- Assessment of glucose-6-phosphate dehydrogenase activity using CareStart G6PD rapid diagnostic test and associated genetic variants in Plasmodium vivax malaria endemic setting in Mauritania
- Spatial distribution of breast cancer in Sudan 2010-2016
- Human-induced fire regime shifts during 19th century industrialization: A robust fire regime reconstruction using northern Polish lake sediments
- Enhanced effectiveness of oil dispersants in destabilizing water-in-oil emulsions
- Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation
- Iron and manganese co-limit growth of the Southern Ocean diatom Chaetoceros debilis
- Bilateral and unilateral load-velocity profiling in a machine-based, single-joint, lower body exercise
- Veterans Health Administration nurses’ training and beliefs related to care of patients with traumatic brain injury
- Supplementation strategies affect the feed intake and performance of grazing replacement heifers
- Active transcutaneous bone conduction hearing implants: Systematic review and meta-analysis
- Parameterization-induced uncertainties and impacts of crop management harmonization in a global gridded crop model ensemble
- Acidification effects on isolation of extracellular vesicles from bovine milk
- GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve
- Mining version history to predict the class instability
- Humpback whale song occurrence reflects ecosystem variability in feeding and migratory habitat of the northeast Pacific
- A phase 2 study of an oral mTORC1/mTORC2 kinase inhibitor (CC-223) for non-pancreatic neuroendocrine tumors with or without carcinoid symptoms
- Asprosin response in hypoglycemia is not related to hypoglycemia unawareness but rather to insulin resistance in type 1 diabetes
- Muscle oxygenation maintained during repeated-sprints despite inspiratory muscle loading
- Reconstructing systematic persistent impacts of promotional marketing with empirical nonlinear dynamics
- Trans-national conservation and infrastructure development in the Heart of Borneo
- Prediction of cardiovascular disease risk among people with severe mental illness: A cohort study
- Data in question: A survey of European biobank professionals on ethical, legal and societal challenges of biobank research
- Hypertensive APOL1 risk allele carriers demonstrate greater blood pressure reduction with angiotensin receptor blockade compared to low risk carriers
- Reconstructing birth in Australopithecus sediba
- Value of contrast-enhanced ultrasound for preoperative assessment of liver reserve function in patients with liver tumors
- Correction: Identifying obesity/overweight status in children and adolescents; A cross-sectional medical record review of physicians’ weight screening practice in outpatient clinics, Saudi Arabia
- Incidence and risk factors of loss to follow-up among HIV-infected children in an antiretroviral treatment program
- Changes in intracellular folate metabolism during high-dose methotrexate and Leucovorin rescue therapy in children with acute lymphoblastic leukemia
- Functional role and evolutionary contributions of floral gland morphoanatomy in the Paleotropical genus Acridocarpus (Malpighiaceae)
- On the efficiency of HIV transmission: Insights through discrete time HIV models
- Metabolic cost calculations of gait using musculoskeletal energy models, a comparison study
- Comparison of molecular profile in triple-negative inflammatory and non-inflammatory breast cancer not of mesenchymal stem-like subtype
- Evolutionary analysis of six chloroplast genomes from three Persea americana ecological races: Insights into sequence divergences and phylogenetic relationships
- Cultural transmission in a food preparation task: The role of interactivity, innovation and storytelling
- Correction: Effects of affective priming through music on the use of emotion words
- Safety and immunogenicity of investigational seasonal influenza hemagglutinin DNA vaccine followed by trivalent inactivated vaccine administered intradermally or intramuscularly in healthy adults: An open-label randomized phase 1 clinical trial
- Estimation of vaccination coverage from electronic healthcare records; methods performance evaluation – A contribution of the ADVANCE-project
- Dietary phytogenics and galactomannan oligosaccharides in low fish meal and fish oil-based diets for European sea bass (Dicentrarchus labrax) juveniles: Effects on gut health and implications on in vivo gut bacterial translocation
- Suicide by hanging: Results from a national survey in Switzerland and its implications for suicide prevention
- Gene dysregulation in peripheral blood of moyamoya disease and comparison with other vascular disorders
- Genome wide genetic dissection of wheat quality and yield related traits and their relationship with grain shape and size traits in an elite × non-adapted bread wheat cross
- Safety and tolerability of artesunate-amodiaquine, artemether-lumefantrine and quinine plus clindamycin in the treatment of uncomplicated Plasmodium falciparum malaria in Kinshasa, the Democratic Republic of the Congo
- Factors influencing the admission decision for Medical Psychiatry Units: A concept mapping approach
- Comparison of post-traumatic changes in circulating and bone marrow leukocytes between BALB/c and CD-1 mouse strains
- Physiological stress reactivity and recovery related to behavioral traits in dogs (Canis familiaris)
- Associations between birth order with mental wellbeing and psychological distress in midlife: Findings from the 1970 British Cohort Study (BCS70)
- Non-steroidal anti-inflammatory drugs use in older adults decreases risk of Alzheimer’s disease mortality
- The cost-effectiveness of neonatal versus prenatal screening for congenital toxoplasmosis
- Ancient technology and punctuated change: Detecting the emergence of the Edomite Kingdom in the Southern Levant
- Flowers as viral hot spots: Honey bees (Apis mellifera) unevenly deposit viruses across plant species
- Randomized control trial of Tools of the Mind: Marked benefits to kindergarten children and their teachers
- Preserving cultural heritage: Analyzing the antifungal potential of ionic liquids tested in paper restoration
- PNPLA3 rs738409 G allele carriers with genotype 1b HCV cirrhosis have lower viral load but develop liver failure at younger age
- Correction: Unraveling the genetic complexity underlying sorghum response to water availability
- Impact of tear metrics on the reliability of perimetry in patients with dry eye
- Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
- Correction: Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge
- Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice
- Patient factors affecting successful linkage to treatment in a cervical cancer prevention program in Kenya: A prospective cohort study
- Is this a man’s world? The effect of gender diversity and gender equality on firm innovativeness
- Procalcitonin to stop antibiotics after cardiovascular surgery in a pediatric intensive care unit—The PROSACAB study
- Temporal trends, predictors, and outcomes of acute kidney injury and hemodialysis use in acute myocardial infarction-related cardiogenic shock
- Quantitation of free glycation compounds in saliva
- Resolving an 87-year-old taxonomical curiosity with the description of Psylla frodobagginsi sp. nov. (Hemiptera: Sternorrhyncha: Psyllidae), a second distinct Psylla species on the New Zealand endemic plant kōwhai
- The effect of cathodal tDCS on fear extinction: A cross-measures study
- Physiological responses to affiliation during conversation: Comparing neurotypical males and males with Asperger syndrome
- Human cord blood (hCB)-CD34+ humanized mice fail to reject human acute myeloid leukemia cells
- Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems
- Continuous norming of psychometric tests: A simulation study of parametric and semi-parametric approaches
- Phylogenetic microbiota profiling in fecal samples depends on combination of sequencing depth and choice of NGS analysis method
- Cost-effectiveness of apixaban compared to other anticoagulants in patients with atrial fibrillation in the real-world and trial settings
- Biochemical profile and in vitro biological activities of extracts from seven folk medicinal plants growing wild in southern Tunisia
- Low genetic differentiation yet high phenotypic variation in the invasive populations of Spartina alterniflora in Guangxi, China
- A mathematical model for designing networks of C-Reactive Protein point of care testing
- Prevalence of hypochondriac symptoms among health science students in China: A systematic review and meta-analysis
- Femtosecond laser induced step-like structures inside transparent hydrogel due to laser induced threshold reduction
- From In Situ to satellite observations of pelagic Sargassum distribution and aggregation in the Tropical North Atlantic Ocean
- The polyether ionophore salinomycin targets multiple cellular pathways to block proliferative vitreoretinopathy pathology
- BRAF V600E and Pten deletion in mice produces a histiocytic disorder with features of Langerhans cell histiocytosis
- HIV prevalence and risk behavior among male and female adults screened for enrolment into a vaccine preparedness study in Maputo, Mozambique
- Sputum microbiota and inflammation at stable state and during exacerbations in a cohort of chronic obstructive pulmonary disease (COPD) patients
- Direct estimation of the parameters of a delayed, intermittent activation feedback model of postural sway during quiet standing
- Occurrence mechanism and coping paths of accidents of highly aggregated tourist crowds based on system dynamics
- Correction: Low Dose Aerosol Fitness at the Innate Phase of Murine Infection Better Predicts Virulence amongst Clinical Strains of Mycobacterium tuberculosis
- Effects of enalapril and paricalcitol treatment on diabetic nephropathy and renal expressions of TNF-α, p53, caspase-3 and Bcl-2 in STZ-induced diabetic rats
- Correction: Public reaction to Chikungunya outbreaks in Italy—Insights from an extensive novel data streams-based structural equation modeling analysis
- Correction: Comparison of neurodegenerative types using different brain MRI analysis metrics in older adults with normal cognition, mild cognitive impairment, and Alzheimer’s dementia
- Intra-individual variability of sleep and nocturnal cardiac autonomic activity in elite female soccer players during an international tournament
- Therapeutic efficacy of equine botulism heptavalent antitoxin against all seven botulinum neurotoxins in symptomatic guinea pigs
- Interleukin 10 knock-down in bovine monocyte-derived macrophages has distinct effects during infection with two divergent strains of Mycobacterium bovis
- Clinical outcomes of bortezomib-based therapy in Taiwanese patients with multiple myeloma: A nationwide population-based study and a single-institute analysis
- Scope and efficacy of the broad-spectrum topical antiseptic choline geranate
- Hematological and biochemical parameters for Chinese rhesus macaque
- Veterinary peer study groups as a method of continuous education—A new approach to identify and address factors associated with antimicrobial prescribing
- Correction: The prognosis of heart failure patients: Does sodium level play a significant role?
- Maternal malaria but not schistosomiasis is associated with a higher risk of febrile infection in infant during the first 3 months of life: A mother-child cohort in Benin
- Systematic identification of facility-based stillbirths and neonatal deaths through the piloted use of an adapted RAPID tool in Liberia and Nepal
- Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis
- Correction: Age, sex and storage time influence hair cortisol levels in a wild mammal population
- Reasons to care: Personal motivation as a key factor in the practice of the professional foster carer in Romania
- Determinants of clinical, functional and personal recovery for people with schizophrenia and other severe mental illnesses: A cross-sectional analysis
- Continuity of care for TB patients at a South African hospital: A qualitative participatory study of the experiences of hospital staff
- EGF receptor stimulation shifts breast cancer cell glucose metabolism toward glycolytic flux through PI3 kinase signaling
- The Better Management of Patients with Osteoarthritis Program: Outcomes after evidence-based education and exercise delivered nationwide in Sweden
- The negative effects of short-term extreme thermal events on the seagrass Posidonia oceanica are exacerbated by ammonium additions
- Pathogen invasion history elucidates contemporary host pathogen dynamics
- Chinese herbal formulae for the treatment of menopausal hot flushes: A systematic review and meta-analysis
- Healthcare resource utilization and costs for multiple sclerosis management in the Campania region of Italy: Comparison between centre-based and local service healthcare delivery
- Deconstruction of central line insertion guidelines based on the positive deviance approach—Reducing gaps between guidelines and implementation: A qualitative ethnographic research
- PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs
- The impact of admission serum lactate on children with moderate to severe traumatic brain injury
- Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface
- Determination of the bruise degree for cherry using Vis-NIR reflection spectroscopy coupled with multivariate analysis
- Genome-wide identification and expression profile analysis of nuclear factor Y family genes in Sorghum bicolor L. (Moench)
- Trends in maternal prepregnancy body mass index (BMI) and its association with birth and maternal outcomes in California, 2007–2016: A retrospective cohort study
- Distribution of the Duffy genotypes in Malaysian Borneo and its relation to Plasmodium knowlesi malaria susceptibility
- Hippocampal connectivity with sensorimotor cortex during volitional finger movements: Laterality and relationship to motor learning
- A self-adaptive deep learning method for automated eye laterality detection based on color fundus photography
- The effect of child marriage on the utilization of maternal health care in Nepal: A cross-sectional analysis of Demographic and Health Survey 2016
- Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)
- Chemical volatiles present in cotton gin trash: A by-product of cotton processing
- PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils
- Differential metabolomics networks analysis of menopausal status
- Experimental study of the temporal profile of breath alcohol concentration in a Chinese population after a light meal
- Association between regional brain volumes and BMI z-score change over one year in children
- Relationships between Potentially Toxic Elements in intertidal sediments and their bioaccumulation by benthic invertebrates
- Long-term outcomes of dialysis in patients with chronic kidney disease and new-onset atrial fibrillation: A population-based cohort study
- Hospital burden of pulmonary arterial hypertension in France
- Understanding the variability of Australian fire weather between 1973 and 2017
- Photon-counting cine-cardiac CT in the mouse
- Ecophysiological impacts of Esca, a devastating grapevine trunk disease, on Vitis vinifera L.
- Black “Reading the Mind in the Eyes” task: The development of a task assessing mentalizing from black faces
- Osmolytes ameliorate the effects of stress in the absence of the heat shock protein Hsp104 in Saccharomyces cerevisiae
- Reliable and robust method for abdominal muscle mass quantification using CT/MRI: An explorative study in healthy subjects
- Validation of the Rainbow Model of Integrated Care Measurement Tools (RMIC-MTs) in renal care for patient and care providers
- Multiple origins and the population genetic structure of Rubus takesimensis (Rosaceae) on Ulleung Island: Implications for the genetic consequences of anagenetic speciation
- Genetic profiling of fatty acid desaturase polymorphisms identifies patients who may benefit from high-dose omega-3 fatty acids in cardiac remodeling after acute myocardial infarction—Post-hoc analysis from the OMEGA-REMODEL randomized controlled trial
- A randomized controlled trial examining the efficacy of an internet-based cognitive behavioral therapy program for adolescents with anxiety disorders
- Optimising outputs from a validated online instrument to measure health-related quality of life (HRQL) in dogs
- Young adults’ perceptions of using wearables, social media and other technologies to detect worsening mental health: A qualitative study
- Characteristics of prescription in 29 Level 3 Neonatal Wards over a 2-year period (2017-2018). An inventory for future research
- Genetic characterization of fall armyworm (Spodoptera frugiperda) in Ecuador and comparisons with regional populations identify likely migratory relationships
- Correction: Lkb1 Deficiency Alters Goblet and Paneth Cell Differentiation in the Small Intestine
- Cardiac resynchronization therapy-heart failure (CRT-HF) clinic: A novel model of care
- Synteny and phylogenetic analysis of paralogous thyrostimulin beta subunits (GpB5) in vertebrates
- Dual-Subpopulation as reciprocal optional external archives for differential evolution
- NGS analysis in Marfan syndrome spectrum: Combination of rare and common genetic variants to improve genotype-phenotype correlation analysis
- Optimization of cataract surgery follow-up: A standard set of questions can predict unexpected management changes at postoperative week one
- Silymarin in non-cirrhotics with non-alcoholic steatohepatitis: A randomized, double-blind, placebo controlled trial
- Clinical evaluation of General Electric new Swiftscan solution in bone scintigraphy on NaI-camera: A head to head comparison with Siemens Symbia
- Pharmacist-led academic detailing improves statin therapy prescribing for Malaysian patients with type 2 diabetes: Quasi-experimental design
- Proton pump inhibitors attenuate myofibroblast formation associated with thyroid eye disease through the aryl hydrocarbon receptor
- Clinical outcomes with neoadjuvant versus adjuvant chemotherapy for triple negative breast cancer: A report from the National Cancer Database
- Identification and characterization of a novel heparan sulfate-binding domain in Activin A longest variants and implications for function
- Wikipedia network analysis of cancer interactions and world influence
- Using path signatures to predict a diagnosis of Alzheimer’s disease
- Development and verification of prediction models for preventing cardiovascular diseases
- Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense
- Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis
- Transience effect in capture-recapture studies: The importance of its biological meaning
- Detailed global modelling of soil organic carbon in cropland, grassland and forest soils
- Oral dosing for antenatal corticosteroids in the Rhesus macaque
- Retraction: Modulation of the Pentose Phosphate Pathway Induces Endodermal Differentiation in Embryonic Stem Cells
- An analysis on HBsAg, Anti-HCV, Anti-HIV½ and VDRL test results in blood donors according to gender, age range and years
- Clinical factors associated with bacterial translocation in Japanese patients with type 2 diabetes: A retrospective study
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- The conditional Fama-French model and endogenous illiquidity: A robust instrumental variables test
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- Correction: In vitro larval rearing protocol for the stingless bee species Melipona scutellaris for toxicological studies
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- Confounding by indication of the safety of de-escalation in community-acquired pneumonia: A simulation study embedded in a prospective cohort
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- Drivers of HIV-1 transmission: The Portuguese case
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- The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity
- A randomized pilot efficacy and safety trial of diazoxide choline controlled-release in patients with Prader-Willi syndrome
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- Effect of PEPFAR funding policy change on HIV service delivery in a large HIV care and treatment network in Nigeria
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- Targeted in-vitro-stimulation reveals highly proliferative multi-virus-specific human central memory T cells as candidates for prophylactic T cell therapy
- Influence of ocean circulation and the Kuroshio large meander on the 2018 Japanese eel recruitment season
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- Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)
- Characteristics of proximal early gastric cancer differentiating distal early gastric cancer
- Retraction: Impacts of rock properties on Danxia landform formation based on lithological experiments at Kongtongshan National Geopark, northwest China
- Correction: SVR12 rates higher than 99% after sofosbuvir/velpatasvir combination in HCV infected patients with F0-F1 fibrosis stage: A real world experience
- Retraction: Plexin-B1 Activates NF-κB and IL-8 to Promote a Pro-Angiogenic Response in Endothelial Cells
- Relationship between cognitive behavioral variables and mental health status among university students: A meta-analysis
- Association between temperature, sunlight hours and alcohol consumption
- Using graph learning to understand adverse pregnancy outcomes and stress pathways
- Correction: The step-to-step transition mode: A potential indicator of first-fall risk in elderly adults?
- Surgical referral systems in low- and middle-income countries: A review of the evidence
- In vivo clearance of nanoparticles by transcytosis across alveolar epithelial cells
- Correction: The Economic Impact of Malignant Catarrhal Fever on Pastoralist Livelihoods
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- Correction: Vascular Endothelial Growth Factor Receptor-2 Couples Cyclo-Oxygenase-2 with Pro-Angiogenic Actions of Leptin on Human Endothelial Cells
- Correction: Plant traits linked to field-scale flammability metrics in prescribed burns in Eucalyptus forest
- Correction: Maturation of three-dimensional, hiPSC-derived cardiomyocyte spheroids utilizing cyclic, uniaxial stretch and electrical stimulation
- Correction: To keep or not to keep? Decision making in adolescent pregnancies in Jamestown, Ghana
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