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Correction: KRIT1 Regulates the Homeostasis of Intracellular Reactive Oxygen Species


Authors:
Published in the journal: PLoS ONE 14(11)
Category: Correction
doi: https://doi.org/10.1371/journal.pone.0223089

Fig 3 is incorrect. The authors have provided a corrected version here. The publisher apologizes for the error.

KRIT1 regulates steady-state levels of intracellular ROS.
Fig. 1. KRIT1 regulates steady-state levels of intracellular ROS.
A–B) Qualitative detection of the steady-state levels of intracellular ROS by fluorescence microscopy. Wild-type (WT), KRIT1−/− (KRIT1−/−) and KRIT1-transduced (Lv-KRIT1 9/6) MEFs grown under standard conditions were analyzed by fluorescence microscopy 20 min after the addition of the cell-permeable redox-sensitive fluorogenic probe DCFH-DA (A) or DHE (B). The images were taken with a fixed short exposure time and a high fluorescence intensity threshold value to avoid saturation, and are representative of several independent experiments. Notice that KRIT1−/− cells (panels b) showed significantly more intense fluorescent signals than WT cells (panels a), indicating that they contained higher levels of ROS. Conversely, ROS levels in KRIT1−/− cells were reduced to near WT levels upon KRIT1 re-expression by lentiviral infection (panels c). Scale bar represents 50 μm. C–H. Quantitative determination of the steady-state levels of intracellular ROS by FACS analysis. Wild-type (WT), KRIT1−/− (K−/−) and three distinct KRIT1−/− cell populations re-expressing KRIT1 at low, medium and high levels, respectively [Lv-KRIT1 2/7 (K2/7), 10/6 (K10/6) and 9/6 (K9/6)], were grown under standard conditions and analyzed by FACS 20 min after the addition of the DCFH-DA (C–F) or DHE (G,H) probes. Representative flow cytometry profiles (C,E,G) and quantitative histograms of the mean fluorescence intensity (M.F.I.) values (D,F,H) of n≥5 independent FACS experiments are shown. M.F.I. values were normalized to spontaneous fluorescence of control cells untreated with the fluorogenic probes (Ctr) and expressed as percentage of KRIT1−/− (K−/−) cells (± SD). *P<0.001 versus KRIT1−/− cells. Notice that KRIT1−/− cells displayed the highest content of intracellular ROS, whereas the re-expression of KRIT1 caused a significant, expression level-dependent decrease in intracellular ROS levels.

Zdroje

1. Goitre L, Balzac F, Degani S, Degan P, Marchi S, Pinton P, et al. (2010) KRIT1 Regulates the Homeostasis of Intracellular Reactive Oxygen Species. PLoS ONE 5(7): e11786. https://doi.org/10.1371/journal.pone.0011786 20668652


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2019 Číslo 11
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