Morphology, phylogeny, and taxonomy of two species of colonial volvocine green algae from Lake Victoria, Tanzania

Autoři: Hisayoshi Nozaki aff001;  Ryo Matsuzaki aff002;  Benedicto Boniphace Kashindye aff004;  Charles Nyarongo Ezekiel aff004;  Sophia Shaban aff004;  Masanobu Kawachi aff002;  Mitsuto Aibara aff005;  Masato Nikaido aff005
Působiště autorů: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan aff001;  Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan aff002;  Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan aff003;  Tanzania Fisheries Research Institute (TAFIRI), Mwanza, Tanzania aff004;  School of Life Science and Technology, Tokyo Institute of Technology, Tokyo, Japan aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0224269


The biodiversity and taxonomy of colonial volvocine green algae are important in ancient lakes in tropical regions. However, few taxonomic studies of these algae have been conducted in African ancient lakes. Here, we describe two species of colonial volvocine green algae in cultures originating from water samples from Lake Victoria, an ancient lake in Africa. One was identified as an undescribed morphological species of Eudorina; E. compacta sp. nov. This new species can be distinguished from other Eudorina species by its compactly arranged vegetative cells that form a hollow ellipsoidal colony. The other was identified as Colemanosphaera charkowiensis. The genus Colemanosphaera is new to Africa.

Klíčová slova:

DNA – Phylogenetic analysis – Taxonomy – Algae – Chloroplasts – Ribosomal RNA – Vacuoles – Lakes


1. Hanschen ER, Marriage TN, Ferris PJ, Hamaji T, Toyoda A, Fujiyama A, et al. The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity. Nat Commun. 2016; 7:11370. doi: 10.1038/ncomms11370 27102219

2. Hamaji T, Kawai-Toyooka H, Uchimura H, Suzuki M, Noguchi H, Minakuchi Y, et al. Anisogamy evolved with a reduced sex-determining region in volvocine green algae. Commun Biol. 2018; 1:17. doi: 10.1038/s42003-018-0019-5 30271904

3. Nozaki H, Mahakham W, Athibai S, Yamamoto K, Takusagawa M, Misumi O, et al. Rediscovery of the ‘ancestral Volvox’ species: morphology and phylogenetic position of Pleodorina sphaerica (Volvocales, Chlorophyceae) from Thailand. Phycologia 2017; 56: 469–475. doi: 10.2216/17-3.1 29375162

4. Nozaki H, Takusagawa M, Matsuzaki R, Misumi O, Mahakham W, Kawachi M. Morphology, reproduction and taxonomy of Volvox dissipatrix (Chlorophyceae) from Thailand, with a description of Volvox zeikusii sp. nov. Phycologia 2019; 58: 192–199. doi: 10.1080/00318884.2018.1540238

5. Nozaki H, Matsuzaki R, Yamamoto K, Kawachi M, Takahashi F. Delineating a new heterothallic species of Volvox (Volvocaceae, Chlorophyceae) using new strains of ‘Volvox africanus’. PLOS ONE 2015; 10: e0142632. doi: 10.1371/journal.pone.0142632 26562165

6. Martens K. Speciation in ancient lakes. Trends Ecol Evol. 1997; 12: 177–182. doi: 10.1016/s0169-5347(97)01039-2 21238028

7. Cristescu ME, Adamowicz SJ, Vaillant JJ, Haffner DG. Ancient lakes revisited: from the ecology to the genetics of speciation. Mol Ecol. 2010; 19: 4837–4851. doi: 10.1111/j.1365-294X.2010.04832.x 20874762

8. Kulikovskiy MS, Lange-Bertalot H, Kuznetsova IV. Lake Baikal: hotspot of endemic diatoms II. In: Lange-Bertalot H. editor. Iconographia Diatomologica Volume: 26. Oberreifenberg: Koeltz Scientific Books; 2015. pp. 1–656.

9. West GS. Some new African species of Volvox. J. Quekett Microsc. Club (ser. 2) 1910; 11: 99–104.

10. Rich F, Pocock MA. Observations on the genus Volvox in Africa. Ann S Afr Mus. 1933; 16: 427–471, pls. 9–24.

11. Smith GM. A comparative study of the species of Volvox. Trans Amer Microsc Soc 1944; 63: 265–310. doi: 10.2307/3223302

12. Starr RC, O’Neil RM, Miller CE III. L-Glutamic acid as a mediator of sexual morphogenesis in Volvox capensis. Proc Natl Acad Sci USA. 1980; 77: 1025–1028. doi: 10.1073/pnas.77.2.1025 16592768

13. McCracken MD, Starr RC. Induction and development of reproductive cells in the K-32 strains of Volvox rousseletii. Arch Protistenk. 1970; 112:262–282.

14. Fabry S, Köhler A, Coleman AW. Intraspecies analysis: comparison of ITS sequence data and gene intron sequence data with breeding data for a worldwide collection of Gonium pectorale. J Mol Evol. 1999; 48: 94–101. doi: 10.1007/pl00006449 9873081

15. Pringsheim EG. Pure cultures of algae. London: Cambridge University Press; 1946.

16. Kawachi M, Ishimoto M, Mori F, Yumoto K, Sato M, Noël M-H. MCC-NIES list of strains, 9th Edition, microbial culture collection at National Institute for Environmental Studies, Tsukuba, Japan; 2013.

17. Nozaki H, Kuroiwa H, Mita T, Kuroiwa T. Pleodorina japonica sp. nov. (Volvocales, Chlorophyta) with bacteria-like endosymbionts. Phycologia 1989; 28: 252–267. doi: 10.2216/i0031-8884-28-2-252.1

18. Nozaki H, Kuroiwa T. Ultrastructure of the extracellular matrix and taxonomy of Eudorina, Pleodorina and Yamagishiella gen. nov. (Volvocaceae, Chlorophyta). Phycologia 1992; 31: 529–541. doi: 10.2216/i0031-8884-31-6-529.1

19. Kimbara R, Isaka N, Matsuzaki R, Kawai-Toyooka H, Kawachi M, Nozaki Morphological and molecular identification of the dioecious “African species” Volvox rousseletii (Chlorophyceae) in the water column of a Japanese Lake based on field-collected and cultured materials. PLoS ONE 2019; 14: e0221632. doi: 10.1371/journal.pone.0221632 31465476

20. Nozaki H, Yamada TK, Takahashi F, Matsuzaki R, Nakada T. New ‘missing link’ genus of the colonial volvocine green algae gives insights into the evolution of oogamy. BMC Evol Biol. 2014; 14: 37. doi: 10.1186/1471-2148-14-37 24589311

21. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol. 2018; 35:1547–1549. doi: 10.1093/molbev/msy096 29722887

22. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783–791. doi: 10.1111/j.1558-5646.1985.tb00420.x 28561359

23. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B et al. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012; 61: 539–542. doi: 10.1093/sysbio/sys029 22357727

24. Isaka N, Kawai-Toyooka H, Matsuzaki R, Nakada T, Nozaki H. Description of two new monoecious species of Volvox sect. Volvox (Volvocaceae, Chlorophyceae), based on comparative morphology and molecular phylogeny of cultured material. J Phycol. 2012; 48: 759–767. doi: 10.1111/j.1529-8817.2012.01142.x 27011093

25. Nozaki H, Misawa K, Kajita T, Kato M, Nohara S, Watanabe MM. Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol Phylog Evol. 2000; 17: 256–268. doi: 10.1006/mpev.2000.0831 11083939

26. Nozaki H. Sexual reproduction in Eudorina elegans (Chlorophyta, Volvocales). Bot Mag Tokyo 1983; 96: 103–110. doi: 10.1007/BF02491095

27. Nozaki H. Eudorina elegans Ehrenberg. In: Yamagishi T, editor. Photomicrographs of the Fresh-water Algae, Volume 3, Tokyo: Uchida Rokakuho; 1994. pp. 22–23.

28. Goldstein M. Speciation and mating behavior in Eudorina. J Protozool. 1964; 11: 317–344. doi: 10.1111/j.1550-7408.1964.tb01762.x

29. Nozaki H, Krienitz L. Morphology and phylogeny of Eudorina minodii (Chodat) Nozaki et Krienitz, comb. nov. (Volvocales, Chlorophyta) from Germany. Eur J Phycol. 2001; 36: 23–28. doi: 10.1080/09670260110001735158

30. Ettl H. Chlorophyta I. Phytomonadina. In: Ettl H, Gerloff J, Mollenhauer D, editors. Süßwasserflora von Mitteleuropa, Volume 9, Stuttgart: Gustav Fischer Verlag; 1983. pp. 1–807. doi: 10.1002/jobm.3620250303

31. Nakada T, Nozaki H. Flagellate Green Algae. In: Wehr J, Sheath R, Kociolek JP, editors. Freshwater Algae of North America (Second Edition). Ecology and Classification; San Diego: Academic Press; 2015. pp. 265–313. doi: 10.1016/B978-0-12-385876-4.00006-2

32. Nozaki H, Kuroiwa T. Pandorina colemaniae sp. nov. (Volvocaceae, Chlorophyta) from Japan. Phycologia 1991; 30: 449–457. doi: 10.2216/i0031-8884-30-5-449.1

33. Fulton AB. Colonial development in Pandorina morum: I. Structure and composition of the extracellular matrix. Develop Biol. 1978; 64: 224–235. doi: 10.1016/0012-1606(78)90074-x 680344

34. Coleman AW. Comparison of Eudorina/Pleodorina ITS sequences of isolates from nature with those from experimental hybrids. Am J Bot. 2002; 89:1523–1530. doi: 10.3732/ajb.89.9.1523 21665754

35. Yamada TK, Miyaji K, Nozaki H. A taxonomic study of Eudorina unicocca (Volvocaceae, Chlorophyceae) and related species, based on morphology and molecular phylogeny. Eur J Phycol. 2018; 43: 317–326. doi: 10.1080/09670260701763484

36. Coleman AW. Lessons from a volvocacean jackpot. Phycologia 2014; 53: 423–425. doi: 10.2216/14-015.1

37. Hu Y, Xing W, Song H, Zhu H, Liu G. Hu Z. Evolutionary analysis of unicellular species in Chlamydomonadales through chloroplast genome comparison with the colonial volvocine algae. Front Microbiol. 2019;10: 1351. doi: 10.3389/fmicb.2019.01351 31275275

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2019 Číslo 11