Region-Specific Activation of mRNA Translation by Inhibition of Bruno-Mediated Repression

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Proteins are often enriched to specific regions within cells via localization of mRNAs. This phenomenon serves a variety of roles, both bringing together factors involved in particular cellular processes to enhance their efficiency, and in restricting proteins that could do harm if deployed at inappropriate positions. In the latter situation, translational repression prevents expression before mRNA localization, and there must be activation mechanisms to inhibit or override repression. How the processes of mRNA localization and translation are coordinated is not well understood, in part because cellular extracts prepared to study mechanisms in vitro do not retain the spatial information present in the intact cell. We developed an in vivo assay to monitor the pattern of translation in the Drosophila oocyte, where several patterning determinants must be localized to specific regions. Using this assay, we showed that repression of translation by the Bruno protein is inhibited, and we could visualize when and where this occurs during oogenesis. Regional activation occurs not only at the site of mRNA localization, but more broadly in a graded fashion, and it does not require an activation element in the mRNA. We also show that Bruno dimerizes, and that dimerization is important for translational activation.

Vyšlo v časopise: Region-Specific Activation of mRNA Translation by Inhibition of Bruno-Mediated Repression. PLoS Genet 11(2): e32767. doi:10.1371/journal.pgen.1004992
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004992

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