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Mouse Y-Linked and Are Expressed during the Male-Specific Interphase between Meiosis I and Meiosis II and Promote the 2 Meiotic Division


The mouse Y chromosome genes Zfy1 and Zfy2 were first identified in the late 1980s during the search for the gene on the Y that triggers male development; they encode proteins that regulate the expression of other genes to which they bind via a ‘zinc finger’ domain. We have now discovered that these genes play important roles during spermatogenesis. Zfy2 proved to be essential for the efficient operation of a ‘checkpoint’ during the first meiotic division that identifies and kills cells that would otherwise produce sperm with an unbalanced chromosome set. Female meiosis, which does not have an equivalent checkpoint, generates a significant proportion of eggs with an unbalanced chromosome set. In the present study we show that Zfy2 also has a major role in ensuring that the second meiotic division occurs, with Zfy1 and a related gene, Zfx, on the X chromosome providing some support. In order to fulfil this function all three genes are expressed in the ‘interphase’ stage between the two divisions. In female meiosis there is no interphase stage between the two meiotic divisions but in this case essential functions during the divisions are supported by stored RNAs, so an interphase is not needed.


Vyšlo v časopise: Mouse Y-Linked and Are Expressed during the Male-Specific Interphase between Meiosis I and Meiosis II and Promote the 2 Meiotic Division. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004444
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004444

Souhrn

The mouse Y chromosome genes Zfy1 and Zfy2 were first identified in the late 1980s during the search for the gene on the Y that triggers male development; they encode proteins that regulate the expression of other genes to which they bind via a ‘zinc finger’ domain. We have now discovered that these genes play important roles during spermatogenesis. Zfy2 proved to be essential for the efficient operation of a ‘checkpoint’ during the first meiotic division that identifies and kills cells that would otherwise produce sperm with an unbalanced chromosome set. Female meiosis, which does not have an equivalent checkpoint, generates a significant proportion of eggs with an unbalanced chromosome set. In the present study we show that Zfy2 also has a major role in ensuring that the second meiotic division occurs, with Zfy1 and a related gene, Zfx, on the X chromosome providing some support. In order to fulfil this function all three genes are expressed in the ‘interphase’ stage between the two divisions. In female meiosis there is no interphase stage between the two meiotic divisions but in this case essential functions during the divisions are supported by stored RNAs, so an interphase is not needed.


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