Positive Feedback of Expression Ensures Irreversible Meiotic Commitment in Budding Yeast

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There are two main types of cell division cycles, mitosis and meiosis. During mitosis, DNA is replicated and then chromosomes segregate, producing two daughter cells with the same ploidy as the progenitor cell. During meiosis, DNA is replicated and then chromosomes undergo two rounds of segregation, producing four gametes with half the ploidy of the progenitor cell. As the cell enters into the meiotic divisions, it irreversibly commits to finishing meiosis and cannot return to mitosis. The molecular mechanisms that define meiotic commitment are not well understood. In this study, we asked whether the regulatory network involved in the transcription of NDT80 has a role in meiotic commitment. Ndt80 is a transcription factor that induces the genes needed for the meiotic divisions. We found that a high-level of Ndt80 activity is required for meiotic commitment; in wildtype cells, this is achieved through a transcriptional positive feedback loop –⁠ a regulatory mechanism in which the Ndt80 protein increases the transcription of its own gene. In the absence of positive feedback, cells escape meiosis inappropriately, resulting in an aberrant cell cycle that causes an increase in genome copy number. This study shows the important role of positive feedback in meiotic commitment and in the maintenance of genome integrity.

Vyšlo v časopise: Positive Feedback of Expression Ensures Irreversible Meiotic Commitment in Budding Yeast. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004398
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004398

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