Accurate chromosome segregation is required for the equal distribution of genetic information to progeny. Failure to equally segregate chromosomes leads to aneuploidy, cell death or cancer. Proteins of the conserved shugoshin family contribute to accurate chromosome segregation in both meiosis and mitosis. The role of shugoshin in protection of centromeric cohesion during meiosis is well understood, but only little is known about shugoshin's function during mitosis. We show that Sgo1 mediates localization of the heterotrimeric phosphatase PP2A-Rts1 to the centromere and that this is in turn important for the efficient recruitment of condensin to the centromere. The failure to load centromeric condensin results in a defect during correction of improper microtubule-kinetochore attachments. Moreover, Sgo1 facilitates the maintenance of a centromeric pool of Aurora B/Ipl1, a conserved mitotic kinase essential for the correction of faulty microtubule-kinetochore attachments. Our results show that Sgo1 operates as a multifunctional hub that coordinates two centromeric functions essential for correct chromosome segregation.
Vyšlo v časopise: Sgo1 Regulates Both Condensin and Ipl1/Aurora B to Promote Chromosome Biorientation. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004411 Kategorie: Research Article prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004411
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