The Effects of Codon Context on Translation Speed

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The central dogma of molecular biology states that DNA is transcribed into RNA, which is translated into protein. The process of translation from messenger RNA (mRNA) into protein by the ribosome is a complicated process involving transfer RNA intermediates with attached amino acids that must recognize 3 base codons in the mRNA sequence to be translated with the correct amino acid. With 4 bases to code for 20 proteins the genetic code is redundant with 61 coding codons and three stop codons. A given amino acid might be coded by one, two, three, four or six different codons. We developed a system that measures the speed of the ribosome in vivo during translation. Using this system, we show that the ability of a given codon to be translated is dependent on its neighboring codons, the codon context effect.

Vyšlo v časopise: The Effects of Codon Context on Translation Speed. PLoS Genet 10(6): e32767. doi:10.1371/journal.pgen.1004392
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004392

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