Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in

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DNA cytosine methylation (5-methylcytosine, 5-meC) plays important roles in genome organization, genomic imprinting, transposon silencing and gene expression. DNA methylation patterns are dynamically controlled by methylation and demethylation reactions during development and reproduction in eukaryotes. In Arabidopsis thaliana, ROS1, a 5-methylcytosine DNA glycosylase, is responsible for active DNA demethylation via a base excision repair pathway. Our previous work showed that a histone acetyltransferase, IDM1, is an important regulator of active DNA demethylation. Here we identified two components, IDL1 and MBD7, as additional novel regulators of active DNA demethylation. Our data suggest that a histone acetyltransferase complex, composed of MBD7, IDL1, IDM2 and IDM1, is required for the recruitment of ROS1 to specific loci for active DNA demethylation. Our findings significantly expand our understanding of the regulatory mechanisms underlying active DNA demethylation in plants.

Vyšlo v časopise: Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in. PLoS Genet 11(5): e32767. doi:10.1371/journal.pgen.1005210
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005210

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