MicroRNA-Dependent Transcriptional Silencing of Transposable Elements in Drosophila Follicle Cells

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The fine-tuning of gene expression required for the normal development of multicellular organisms involves small RNAs that are called microRNAs (miRNAs). MiRNAs can reduce the stability or the activity of the many cellular messenger RNAs that contain miRNA complementary sequences. In animal gonads, the harmful expression and proliferation of genomic parasites, such as transposable elements, is prevented by a similar, sequence homology-based silencing mechanism that involves a different class of small RNAs, the Piwi-interacting RNAs (piRNAs). We report here that, in Drosophila somatic ovarian tissues, two miRNAs, miR-14 and miR-34, are required for the accumulation of piRNAs that prevent the expression of transposable elements and, probably, the subsequent invasion of the germinal genome. On the other hand, we found that other sources of piRNA production, such as the 3' end of genes, are miRNA-independent, suggesting the existence of variations in the piRNA biogenesis pathways depending on the piRNA genomic origin. Our results therefore highlight a novel miRNA function in the maintenance of genome stability through piRNA-mediated TE repression.

Vyšlo v časopise: MicroRNA-Dependent Transcriptional Silencing of Transposable Elements in Drosophila Follicle Cells. PLoS Genet 11(5): e32767. doi:10.1371/journal.pgen.1005194
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005194

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