Comparative Genomic Analysis of N-Fixing and Non-N-Fixing spp.: Organization, Evolution and Expression of the Nitrogen Fixation Genes

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We sequenced the genomes of 11 N2-fixing Paenibacillus strains and demonstrated the genomic diversity of the genus Paenibacillus by comparing these strains to each other and to 20 other strains (4 N2-fixing and 16 non-N2-fixing strains) that were sequenced previously. Phylogenetic analysis of the concatenated 275 single-copy core genes suggests that ancestral Paenibacillus did not fix nitrogen and the N2-fixing strains fall into two sub-groups, which were likely originated from a N2-fixing common ancestor. A minimal and compact nif cluster comprising nine nif genes encoding Mo-nitrogenase is highly conserved in the 15 N2-fixing strains. Variations in the nif cluster and in the chromosomal regions surrounding the nif cluster between two sub-groups imply at least two independent acquisitions with insertion of distinct nif cluster variants in different genomic sites of Paenibacillus in early evolutionary history. Phylogeny of the concatenated NifHDK sequences suggests that Paenibacillus and Frankia are sister groups. The nif cluster, a functional unit for nitrogen fixation, was lost, producing some non-N2-fixing strains. There were recent events of acquisition of vnf and anf genes, causing further diversification of some strains. The evolution of nitrogen fixation in Paenibacillus involves a mix of gain, loss, HGT and duplication of nif/anf/vnf genes.

Vyšlo v časopise: Comparative Genomic Analysis of N-Fixing and Non-N-Fixing spp.: Organization, Evolution and Expression of the Nitrogen Fixation Genes. PLoS Genet 10(3): e32767. doi:10.1371/journal.pgen.1004231
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004231

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