GC-Rich DNA Elements Enable Replication Origin Activity in the Methylotrophic Yeast

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Genome duplication in eukaryotes initiates at loci called replication origins. Origins in most budding and fission yeasts are A/T-rich DNA sequences, while metazoan origins are G/C-rich and are often associated with promoters. Here we have globally mapped replication origins and nucleosome positions in an industrially important methylotrophic yeast, Pichia pastoris. We show that P. pastoris has two general classes of origins—A/T-rich origins resembling those of most other yeasts, and a novel, G/C-rich class, that appear more robust and are associated with promoters. P. pastoris is the first known species using two kinds of origins and the first known budding yeast to use a G/C-rich origin motif. Additionally, the G/C-rich motif matches one of the motifs annotated as binding sites of the human Hsf1 transcriptional regulator suggesting that in this species there may be a link between transcriptional regulation and DNA replication initiation.

Vyšlo v časopise: GC-Rich DNA Elements Enable Replication Origin Activity in the Methylotrophic Yeast. PLoS Genet 10(3): e32767. doi:10.1371/journal.pgen.1004169
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004169

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