Identification of Interphase Functions for the NIMA Kinase Involving Microtubules and the ESCRT Pathway

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All organisms have to integrate cell growth, and often the polarization of cell growth, with the rate of progression through the cell cycle. One of the most highly polarized modes of growth found in nature is displayed by the ubiquitous filamentous fungi. How the regulation of mitotic divisions is linked to polarized growth remains a mystery, but might involve mitotic regulators. One key mitotic regulator identified in the model filamentous fungus Aspergillus nidulans is the NIMA kinase, the founding member of the Nek family of protein kinases. This kinase is known to play mitotic specific roles within nuclei. Our genetic studies reported here reveal unexpected interactions between NIMA and six components of a pathway required for the turnover of cell membrane proteins (the ESCRT pathway) showing NIMA and ESCRT components are required together for normal polarized growth. This suggests NIMA has non-nuclear interphase functions. Further supporting this possibility, cell biological studies link NIMA to both cell tip growth and the modulation of interphase microtubule functions. Collectively the findings therefore extend the functions of the NIMA kinase beyond mitosis to include the regulation of normal polarized cell growth, suggesting this kinase might help integrate these two key aspects of growth and development.

Vyšlo v časopise: Identification of Interphase Functions for the NIMA Kinase Involving Microtubules and the ESCRT Pathway. PLoS Genet 10(3): e32767. doi:10.1371/journal.pgen.1004248
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004248

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