The Kil Peptide of Bacteriophage λ Blocks Cytokinesis via ZipA-Dependent Inhibition of FtsZ Assembly

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Bacterial antibiotic resistance is a serious concern, particularly its role in hospital-acquired infection. Viruses that infect bacteria (bacteriophage) can kill their host, and some prevent the bacterial cell from reproducing during that process. Since their discovery, phage have been considered a potential tool against bacterial infection, but little is known regarding how phage-encoded factors may inhibit bacterial cell division. Understanding the interaction between phage factors and the targeted host systems is therefore a critical research goal. Our report focuses on E. coli and λ, a well-studied phage that infects it. λ contains a gene, kil, whose expression prevents E. coli from dividing, causing cells to grow into long filaments that die. Here we report that Kil protein prevents an essential bacterial protein, FtsZ, from properly assembling into the structure needed for cell division. Our data show that Kil can inhibit FtsZ assembly directly in vitro, but that ZipA, another essential cell division protein, enhances its activity on FtsZ in vivo. The results of our study elucidate one way that a phage naturally inhibits bacterial reproduction, which could serve as a target for rational antibiotic design.

Vyšlo v časopise: The Kil Peptide of Bacteriophage λ Blocks Cytokinesis via ZipA-Dependent Inhibition of FtsZ Assembly. PLoS Genet 10(3): e32767. doi:10.1371/journal.pgen.1004217
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1004217

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