Osmolytes ameliorate the effects of stress in the absence of the heat shock protein Hsp104 in Saccharomyces cerevisiae


Autoři: Arnab Bandyopadhyay aff001;  Indrani Bose aff002;  Krishnananda Chattopadhyay aff001
Působiště autorů: Structural Biology & Bio-Informatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India aff001;  Department of Biology, Western Carolina University, Cullowhee, North Carolina, United States of America aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0222723

Souhrn

Aggregation of the prion protein has strong implications in the human prion disease. Sup35p is a yeast prion, and has been used as a model protein to study the disease mechanism. We have studied the pattern of Sup35p aggregation inside live yeast cells under stress, by using confocal microscopy, fluorescence activated cell sorting and western blotting. Heat shock proteins are a family of proteins that are produced by yeast cells in response to exposure to stressful conditions. Many of the proteins behave as chaperones to combat stress-induced protein misfolding and aggregation. In spite of this, yeast also produce small molecules called osmolytes during stress. In our work, we tried to find the reason as to why yeast produce osmolytes and showed that the osmolytes are paramount to ameliorate the long-term effects of lethal stress in Saccharomyces cerevisiae, either in the presence or absence of Hsp104p.

Klíčová slova:

Biology and life sciences – Organisms – Eukaryota – Fungi – Yeast – Saccharomyces – Saccharomyces cerevisiae – Cell biology – Osmotic shock – Cell processes – Cellular stress responses – Heat shock response – Molecular biology – Macromolecular structure analysis – Protein folding – Biochemistry – Proteins – Protein structure – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Yeast and fungal models – Microscopy – Light microscopy – Confocal microscopy – Physical sciences – Chemistry – Chemical compounds – Organic compounds – Carbohydrates – Disaccharides – Trehalose – Organic chemistry – Medicine and health sciences – Infectious diseases – Prion diseases – Zoonoses


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