Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide

Autoři: Aung B. Bo aff001;  Jae D. Kim aff002;  Young S. Kim aff002;  Hun T. Sin aff001;  Hye J. Kim aff002;  Botir Khaitov aff001;  Young K. Ko aff002;  Kee W. Park aff001;  Jung S. Choi aff002
Působiště autorů: Department of Crop Science, Chungnam National University, Daejeon, Korea aff001;  Eco-friendly and New Materials Research Group, Korea Research Institute of Chemical Technology, Daejeon, Korea aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
prolekare.web.journal.doi_sk: 10.1371/journal.pone.0222933


Bioactive herbicidal compounds produced by soil microorganisms might be used to creating a bioherbicide for biological weed control. A total of 1,300 bacterial strains were isolated and screened for herbicidal activity against grass and broadleaf weeds. Among primarily selected 102 strains, the herbicidal activity of bacterial fermentation broths from the following three isolates strain-101, strain-128, and strain-329 reduced the growth of D. sanguinalis by 66.7%, 78.3%, and 100%, respectively as compared with control. Phylogenetic analysis of 16S rRNA gene sequencing determined that the strain-329 has 99% similarity to Streptomyces anulatus (HBUM 174206). The potential bioherbicidal efficacy of Streptomyces strain-329 was tested on grass and broadleaf weeds for phytotoxic activity through pre- and post-emergence applications. At pre-emergence application, the phytotoxic efficacy to D. sanguinalis and S. bicolor on seed germination were 90.4% and 81.3%, respectively at the 2x concentration, whereas in the case of Solanum nigrum, 85.2% phytotoxic efficacy was observed at the 4x concentration. The efficacy of Streptomyces strain-329 was substantially higher at post-emergence application, presenting 100% control of grass and broadleaf weeds at the 1x concentration. Two herbicidal compounds coded as 329-C1 and 329-C3 were extracted and purified by column chromatography and high-performance liquid chromatography methods. The active compound 329-C3 slightly increased leaf electrolytic leakage and MDA production as concentration-dependent manner. These results suggest that new Streptomyces sp. strain-329 produced bioherbicidal metabolites and may provide a new lead molecule for production an efficient bioherbicide to regulate grass and broadleaf weeds.

Klíčová slova:

Grasses – Chlorophyll – Leaves – Phylogenetic analysis – Ribosomal RNA – Streptomyces – Weeds – Fermentation


1. Vasilakoglou I, Eleftherohorinos I, Dhima K. Propanil-resistant barnyard grass (Echinochloa crus-galli) biotypes found in Greece. Weed Technol. 2000; 14: 524–529.

2. Yasuor H, Tenbrook P, Tjeerdema R. Responses to clomazone and 5-ketoclomazone by Echinochloa phyllopogon resistant to multiple herbicides in California rice fields. Pest Manag Sci. 2008; 64: 1031–1039. doi: 10.1002/ps.1604 18493924

3. Lee B, Kim JD, Kim YS, Ko YK, Yon GH, Kim CJ. Identification of Streptomyces scopuliridis KR-001 and its herbicidal characteristics. Weed Turfgrass Sci. 2013; 2: 38–46.

4. Dhanasekaran D, Ambika K, Thajuddin N, Panneerselvam A. Allelopathic effect of actinobacterial isolates against selected weeds. Archi Phytopatho Plant Prot. 2012; 45: 505–521.

5. Isrvan U. Transforming natural products into natural pesticides experience and expectations. Phytoparasitica. 2002; 30: 1–4.

6. Subhashini DV. Bioherbicidal activity of Streptomyces spp isolated from tobacco rhizosphere against certain dicot and monocot weeds. Indian J Agr Sci. 2012; 82(12): 12–14.

7. Daniel JJ, Zabot GL, Tres MV, Harakava R, Kuhn RC, Mazutti MA. Fusarium fujikuroi: A novel source of metabolites with herbicidal activity. Biocatal Agric Biotechnol. 2018; 14: 314–320.

8. Cai X, Gu M. Bioherbicides in organic horticulture. Horticul. 2016; 2: 3.

9. Dayan FE, Ferreria D, Wang YH, Khan IA, McInroy JA, Pan ZQ. A pathogenic fungi diphenyl ether phytotoxin targets plant enoyl (acetyl carrier protein) reductase. Plant Physiol. 2008; 147: 1062–1071. doi: 10.1104/pp.108.118372 18467464

10. Ji YG, Qiu Jian, Li CG, Huang YL, Zhang LP, Shi YM. The status and potential using of bacterial herbicides. Biotechnol. 2006; 16: 88–90.

11. Basilio A, Gonzalez I, Vicente MF, Gorrochategui J, Cabello A, Gonzalez A, et al. Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. J. Appl. Microbiol. 2003; 95: 814–823. 12969296

12. Won OJ, Kim YT, Choi JS, Oh TK, Shinogi Y, Park KW. 2016. Herbicidal activity and mode of action of Streptomyces scopuliridis metabolites. J. Fac. Agric. Kyushu Univ. 61, 47–51.

13. Rana SS, Rana MC. Principles and Practices of Weed Management. Department of Agronomy, College of Agriculture, CSK Himachal Pradesh KrishiVishvavidyalaya, Palampur, 2016. pp. 138.

14. Todero I, Confortin TC, Luft L, Brun T, Ugalde GA, de Almeida TC, et al. Formulation of a bioherbicide with metabolites from Phoma sp. Sci hort. 2018; 241: 285–292.

15. Christy AL, Herbst KA, Kostka SJ, Mullen JP, Carlson PS. Synergizing weed biocontrol agents with chemical herbicides. In: Duke SO, Menm JJ, Plimmer JR, editors. Pest Control with Enhanced Environmental Safety. Washington DC: American Chemical Society; 1993. pp. 87–100.

16. Nakajimam M, Itoi K, Takamatsu Y, Kinoshita T, Okazaki T, Kawakubo K. Hydantocidin: a new compound with herbicidal activity from Streptomyces hygroscopicus. J. Antibiot. 1991; 44: 293–300. doi: 10.7164/antibiotics.44.293 2026555

17. Xu W, Tao L, Gu X, Shen X, Yuan S. Herbicidal activity of the metabolite SPRI-70014 from Streptomyces griseolus. Weed Sci. 2009; 57: 547–553.

18. Ha S, Lee KJ, Lee SI, Gwak HJ, Lee JH, Kim TW, et al. Optimization of Herbicidin A production in submerged culture of Streptomyces scopuliridis M40. J Microbiol Biotechnol. 2017; 27: 947–955. doi: 10.4014/jmb.1611.11005 28237998

19. Sanghyum H, Keon JL, Sang IL, Hyun JG, Jong HL, Tae WK, et al. Optimization of herbicidin a production in submerged culture of Streptomyces scopuliridis M40. J. Microbio. Biot. 2017; 27: 947–955.

20. Valanarasu M, Duraipandiyan V, Agastian P, Ignacimuthu S. In vitro antimicrobial activity of Streptomyces from Western Ghats rock soil (India). J Mycol Med. 2009: 19: 22–28.

21. Hayakawa M, Nonomura H. Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomyces. J Ferment Tech. 1987; 65: 501–509.

22. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Bio. Evol. 1987; 4: 406–425.

23. Lee HB, Kim DJ, Kim JS, Hong KS, Cho KY. A bleaching herbicidal activity of methoxyhygromycin (MHM) produced by an actinomycete strain Streptomyces sp. 8E-12. Lett Appl Microbiol. 2003; 36: 387–391. 12753247

24. Hiscox JD, Israelstam GF. A method for the extraction of chlorophyll from leaf tissues without maceration. Can J Bot. 1979; 57(12): 1332–1334.

25. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52: 302. doi: 10.1016/s0076-6879(78)52032-6 672633

26. DeFrank J, Purnam AR. Screening procedures to identify soil-borne actinomyces that can produce herbicidal compounds. Weed Sci. 1985; 33: 271–274.

27. Heisey RM, DeFrank J, Putnam AR. A survey of soil microorganisms for herbicidal activity. In: Thompson AC, editor. The Chemistry of Allelopathy, ACS Symposium series No. 268. Washington DC: American Chemical Society; 1985. pp. 337–349.

28. El-Sayed MG, El-Aziz ZKA, Abouzaid AM. Efficacy of extracellular metabolite produced by Streptomyces levis Strain LX-65 as a potential herbicidal. J Ameri Sci. 2014; 10; 11.

29. Sondhia S, Saxena NK. Allelopathic effect of Xanthinum strumarium L. on some weeds. J. Geobios. 2003; 30; 173–176.

30. Harding DP, Raizada MN. Controlling weeds with fingi, bacteria and viruses: a review. Front Plant Sci. 2015; 6: 659. doi: 10.3389/fpls.2015.00659 26379687

31. Ghorbani R, Leifert C, Seel W. Biological control of weeds with antagonistic plant pathogens. Adv. Agron. 2005; 86: 191–225.

32. Stergiopoulos I, Collemare J, Mehrabi R, De Wit P. Phytotoxic secondary metabolites and peptides produced by plant pathogenic Dothideomycete fungi. Fems Microbiol. Rev. 2013; 37: 67–93. doi: 10.1111/j.1574-6976.2012.00349.x 22931103

33. Daguerre Y, Siegel K, Edel-Hermann V, Steinberg C. Fungal proteins and genes associated with biocontrol mechanisms of soil-borne pathogens: a review. Fungal Biol. Rev. 2014; 28: 97–125.

34. Walton JD. Host-selective toxins: Agents of compatibility. Plant Cell. 1996; 8: 1723–1733. doi: 10.1105/tpc.8.10.1723 8914323

35. Yandoc CB, Charudattan R, Shilling DG. Evaluation of fungal pathogens as biological control agents for cogongrass (Imperata cylindrica). Weed Technol. 2005; 19: 19–26.

36. Hoagland RE, Boyette CD, Stetina KC, Jordan RH. Bioherbicidal efficacy of a Myrothecium verrucaria-sector on several plant species. Am J Plant Sci. 2016; 7(16): 2376–2389.

37. Kuster E. Morphological and physiological aspects of the taxonomy of Streptomyces. Microbiol Espanola. 1963; 16: 193–202.

38. Hwang EI, Bong SY, Sung WC, Jin SK, Se JL, Jae SM, et al. Isolation of Sangivamycin from Streptomyces sp. A6497 and its herbicidal activity. J Microbiol Biotechnol. 2005; 15: 434–437.

39. Igarashi M, Kiroshita N, Idada T, Kameda M, Hamada M, Takeuschi T. Resormycin, a novel herbicidal and antifungal antibiotic produced by a strain of Streptomyces platensis. J Antibiot. 1997; 50: 1020–1025. doi: 10.7164/antibiotics.50.1020 9510908

40. Chun JC, Kim JC, Hwang IT, Kim SE. Acteoside from Rehmannia glutinosa nullifies paraquat activity in Cucumis sativus. Pest Biochem Physiol. 2002; 72: 153–159.

41. El-Sayed W. Biological control of weeds with pathogens: currents status and future trends. J Plant Dis Protect. 2005; 112: 209–221.

Článok vyšiel v časopise


2019 Číslo 9

Najčítanejšie v tomto čísle

Tejto téme sa ďalej venujú…


Zvýšte si kvalifikáciu online z pohodlia domova

Faktory ovlivňující léčbu levotyroxinem
nový kurz

Kurz originály vs. generika

Autori: MUDr. Petr Výborný, CSc., FEBO

Autori: MUDr. Jiří Horažďovský, Ph.D

Klinická farmakokinetika betablokátorů

Všetky kurzy
Zabudnuté heslo

Nemáte účet?  Registrujte sa

Zabudnuté heslo

Zadajte e-mailovú adresu, s ktorou ste vytvárali účet. Budú Vám na ňu zasielané informácie k nastaveniu nového hesla.


Nemáte účet?  Registrujte sa