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The lncRNA Controls Cryptococcal Morphological Transition


The involvement of non-protein regulators in developmental processes in higher eukaryotes is an area that has come to light more recently. Earlier known as the dark matter of the genome, the non-protein coding genes are now recognized for their important regulatory roles in the life of eukaryotes. Using forward genetic screen, we identified RZE1 as a lncRNA with key function in regulating morphogenesis in Cryptococcus. We further discovered that RZE1 regulates the transcription and transcript export of ZNF2, which encodes the key morphogenesis transcription factor. RZE1 is the first functionally characterized lncRNA in a human fungal pathogen. Given the potential large number of lncRNAs in Cryptococcus and other fungal pathogens, the RZE1-ZNF2 regulatory system could serve as a paradigm for the investigation of lncRNAs in development and virulence in eukaryotic pathogens.


Vyšlo v časopise: The lncRNA Controls Cryptococcal Morphological Transition. PLoS Genet 11(11): e32767. doi:10.1371/journal.pgen.1005692
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005692

Souhrn

The involvement of non-protein regulators in developmental processes in higher eukaryotes is an area that has come to light more recently. Earlier known as the dark matter of the genome, the non-protein coding genes are now recognized for their important regulatory roles in the life of eukaryotes. Using forward genetic screen, we identified RZE1 as a lncRNA with key function in regulating morphogenesis in Cryptococcus. We further discovered that RZE1 regulates the transcription and transcript export of ZNF2, which encodes the key morphogenesis transcription factor. RZE1 is the first functionally characterized lncRNA in a human fungal pathogen. Given the potential large number of lncRNAs in Cryptococcus and other fungal pathogens, the RZE1-ZNF2 regulatory system could serve as a paradigm for the investigation of lncRNAs in development and virulence in eukaryotic pathogens.


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