Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in

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The cell shape changes that underlie cell division are some of the most fundamental changes in cell morphology. Here, we show that a common membrane trafficking pathway is required for both the cell lengthening that occurs during anaphase, and the physical separation of a cell into two equal daughter cells. We measure and define the periods of surface area increase during cell division in Drosophila male germline cells, and demonstrate that subunits of the exocyst tethering complex are required for this process. Invagination of the cleavage furrow fails at an early stage in exocyst mutant spermatocytes, suggesting that membrane addition is part of the initial ingression mechanism. In the absence of exocyst complex function, vesicular trafficking pathways are disrupted, leading to enlarged cytoplasmic membrane stores, and disruption of Golgi architecture. In addition, a vesicular Rab protein, Rab11, biochemically associates with the exocyst complex subunit Sec5. These results suggest that remodeling of the plasma membrane and targeted increases in surface area are an active part of the fundamental mechanisms that permit eukaryotic cell division to occur.

Vyšlo v časopise: Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in. PLoS Genet 11(11): e32767. doi:10.1371/journal.pgen.1005632
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.pgen.1005632

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