Host ESCRT Proteins Are Required for Bromovirus RNA Replication Compartment Assembly and Function

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Positive-strand RNA {(+)RNA} viruses cause numerous human, animal, and plant diseases. (+)RNA viruses reorganize host intracellular membranes to assemble their RNA replication compartments, which are mini-organelles featuring the close association of both viral and host components. To further understand the role of host components in forming such RNA replication compartments, we used brome mosaic virus (BMV), a well characterized model virus, to study some common features of (+)RNA virus RNA replication. We show that knocking out several components of the cellular Endosomal Complex Required for Transport (ESCRT) machinery resulted in parallel defects in BMV RNA replication and replication compartment formation, whereas other ESCRT components affected RNA replication independently of replication compartment formation. Deleting a subset of ESCRT proteins altered the frequency of replication compartment formation but had no effect on the size of these compartments, whereas a second subset affected RNA replication independently of replication compartment formation. Moreover, BMV’s interaction with the ESCRT machinery appears to be distinct from that reported for other viruses and from the ESCRT requirements for forming vesicles in cellular multivesicular bodies. These findings further illuminate the remarkable abilities of positive-strand RNA viruses to integrate viral and host protein functions to remodel membranes, and suggest potentially potent new ways to control such viruses.

Vyšlo v časopise: Host ESCRT Proteins Are Required for Bromovirus RNA Replication Compartment Assembly and Function. PLoS Pathog 11(3): e32767. doi:10.1371/journal.ppat.1004742
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004742

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