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Exome and Transcriptome Sequencing of Identifies a Locus That Confers Resistance to and Alters the Immune Response


Within mosquito populations, genetic differences between individuals affect their ability to transmit human diseases such as malaria, dengue fever, and lymphatic filariasis. In the mosquito Aedes aegypti, some individuals are genetically resistant to Brugia malayi, a mosquito-vectored parasite that causes a debilitating tropical disease called lymphatic filariasis. To characterize the genetic basis of resistance, we identified resistant and susceptible mosquitoes from a wild Kenyan population, and sequenced the protein-coding region of their genomes (the exome). This allowed us to locate a single region of the mosquito genome that is causing resistance and to identify genes that may be controlling the trait. To understand the mechanisms of resistance, we measured gene expression. The susceptible mosquitoes have reduced expression of immunity genes after they are infected with B. malayi, including genes known to kill this group of parasites. This is possibly because their immune response is being suppressed by the parasites. We conclude that resistance is controlled by a single locus and show that resistance results in an increased immune response.


Vyšlo v časopise: Exome and Transcriptome Sequencing of Identifies a Locus That Confers Resistance to and Alters the Immune Response. PLoS Pathog 11(3): e32767. doi:10.1371/journal.ppat.1004765
Kategorie: Research Article
prolekare.web.journal.doi_sk: https://doi.org/10.1371/journal.ppat.1004765

Souhrn

Within mosquito populations, genetic differences between individuals affect their ability to transmit human diseases such as malaria, dengue fever, and lymphatic filariasis. In the mosquito Aedes aegypti, some individuals are genetically resistant to Brugia malayi, a mosquito-vectored parasite that causes a debilitating tropical disease called lymphatic filariasis. To characterize the genetic basis of resistance, we identified resistant and susceptible mosquitoes from a wild Kenyan population, and sequenced the protein-coding region of their genomes (the exome). This allowed us to locate a single region of the mosquito genome that is causing resistance and to identify genes that may be controlling the trait. To understand the mechanisms of resistance, we measured gene expression. The susceptible mosquitoes have reduced expression of immunity genes after they are infected with B. malayi, including genes known to kill this group of parasites. This is possibly because their immune response is being suppressed by the parasites. We conclude that resistance is controlled by a single locus and show that resistance results in an increased immune response.


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